Mesothelin (MSLN) (NM_005823) Human Untagged Clone

CAT#: SC110135

MSLN (untagged)-Human mesothelin (MSLN), transcript variant 1



  "NM_005823" in other vectors (6)

CNY 4,640.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
MSLN mouse monoclonal antibody, clone OTI1G8 (formerly 1G8)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MPF; SMRP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110135 sequence for NM_005823 edited (data generated by NextGen Sequencing)
ATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGC
CTCCTGTTCCTGCTCTTCAGCCTCGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAG
ACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGC
CTCTCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAG
CGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCAAGCTCTCAACAGAGCAG
CTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTG
GACCTGCTGCTATTCCTCAACCCAGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTC
TTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAG
CGGCTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCT
GATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGCGCTTTGTGGCCGAGTCG
GCCGAAGTGCTGCTACCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCAGGACCAGCAG
GAGGCAGCCAGGGCGGCTCTGCAGGGCGGGGGACCCCCCTACGGCCCCCCGTCGACATGG
TCTGTCTCCACGATGGACGCTCTGCGGGGCCTGCTGCCCGTGCTGGGCCAGCCCATCATC
CGCAGCATCCCGCAGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCC
TGGCGGCAGCCTGAACGGACCATCCTCCGGCCGCGGTTCCGGCGGGAAGTGGAGAAGACA
GCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGCCTCATCTTCTACAAGAAG
TGGGAGCTGGAAGCCTGCGTGGATGCGGCCCTGCTGGCCACCCAGATGGACCGCGTGAAC
GCCATCCCCTTCACCTACGAGCAGCTGGACGTCCTAAAGCATAAACTGGATGAGCTCTAC
CCACAAGGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCTTCCTCAAGATGAGC
CCTGAGGACATTCGCAAGTGGAATGTGACGTCCCTGGAGACCCTGAAGGCTTTGCTTGAA
GTCAACAAAGGGCACGAAATGAGTCCTCAGGTGGCCACCCTGATCGACCGCTTTGTGAAG
GGAAGGGGCCAGCTAGACAAAGACACCCTAGACACCCTGACCGCCTTCTACCCTGGGTAC
CTGTGCTCCCTCAGCCCCGAGGAGCTGAGCTCCGTGCCCCCCAGCAGCATCTGGGCGGTC
AGGCCCCAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCCTCTATCCCAAGGCC
CGCCTTGCTTTCCAGAACATGAACGGGTCCGAATACTTCGTGAAGATCCAGTCCTTCCTG
GGTGGGGCCCCCACGGAGGATTTGAAGGCGCTCAGTCAGCAGAATGTGAGCATGGACTTG
GCCACGTTCATGAAGCTGCGGACGGATGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAG
AAACTTCTGGGACCCCACGTGGAGGGCCTGAAGGCGGAGGAGCGGCACCGCCCGGTGCGG
GACTGGATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGCTGGGGCTACAGGGC
GGCATCCCCAACGGCTACCTGGTCCTAGACCTCAGCATGCAAGAGGCCCTCTCGGGGACG
CCCTGCCTCCTAGGACCTGGACCTGTTCTCACCGTCCTGGCACTGCTCCTAGCCTCCACC
CTGGCCTGA

Clone variation with respect to NM_005823.5
>OriGene 5' read for NM_005823 unedited
CCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG
CTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTC
GGCACGAGCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCC
CTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCTCGGATGGGTGCAGCCCTCGAGGACCCTG
GCTGGAGAGACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAAC
ATTTCCAGCCTCTCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTG
AGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCAAGCTCTCA
ACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCC
CTCCCATTGGACCTGCTGCTATTCCTCAACCCAGATGCGTTCTCGGGGCCCCAGGCCTGC
ACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCC
GAGCGACAGCGGCTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGNGGTCTCTGCTG
AGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGCGCTTTGTG
GCCGAGTNCGCCGAAGTGCTGCTACCCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCA
NGACCAGCANNGAGCANCCAGGGCGCTTCNGGGNGGGGGGGGGACCCCCCTACGGCCCCC
CGTCGACATGGTCTGTCTCCACGATGGACGCTCCTGCGGGGCCTGCTNGCCGTGCTGGGC
CAGCCCATCACCGCAGCATCCCGCAGGGCATCGTGGCCCGCTGGCGGCAACGCTCCTCTC
GGGACCATTCTTGGCGCAGCTGAACGA
>OriGene 3' read for NM_005823 unedited
GCCTTACGACCCGCGGCCCGCAATCTAGAGTCCGAGCCCCCCCCCCCCCCCCCCCCCCCT
TCAACGTGTCTGCAGGGGGCATGTTCCCGTTTACTGAGCGCGAGTTCTCTTGGGGTGGAA
CGGGGACCACCCGTGCCTGCTCCTGGCCAGGCGGGGATCCCCAGCAGGGCTGGGCCCAGC
AAGGGAGTGGGGCCCTCAGGCCAGGGTGGAGGCTAGGACCAGTGCCAGGACGGTGAAAAC
AGGTCCAGGTCCTAAGAGGCAGGGCGTCCCCGAGAGGGCCTCTTGCATGCTGAGGTCTAG
GACCAGGTACCCGTTGGGGATGCCGCCCTGTAGCCCCAGCCCCAGCGTGTCCAGGTCGTC
CTGCCGCTGCCGTAAGATCCAGTCCCGCACCGGGCGGTGCCGCTCCTCCGCCTTCAGGCC
CTCCACGTGGGGTCCCAAAAGTTTCTGCACCTCAGCCACAGTCAACGGCAGCACCGCATC
CGTCCGCAGCTTCATGAACGTGGCCAAGTCCATGCTCACATTCTGCTGACTGAGCGCCTT
CAAATCCTCCGTGGGGGCCCCACCCAGGAAGGACTGGATCTTCACGAAGTATTCGGACCC
GTTCATGTTCTGGAAAGCAAGGCGGGCCTTGGGATAAAGGACGTCCAGCTGCCTTGGGTC
ACACGTGTCCAGGTCCTGGGGCCTGACCGCCCAAATGCTGCTGGGGGGCACGGAACTCAT
CTCCTCGGGGCCTGAGGAGCACAGGTACCCAGGGTAAAAAGCGGTCAAGGTGTCCAAGGT
GTCTTTTTCTAACTGCCCCCTTA
Restriction Sites NotI-NotI     
ACCN NM_005823
Insert Size 2400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005823.4, NP_005814.2
RefSeq Size 2052 bp
RefSeq ORF 1869 bp
Locus ID 10232
UniProt ID Q13421
Protein Families Druggable Genome, Secreted Protein, Transmembrane
Gene Summary This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Both variants 1 and 3 encode the same isoform isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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