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RAD50 (NM_005732) Human Untagged Clone
CAT＃: SC110122
RAD50 (untagged)-Human RAD50 homolog (S. cerevisiae) (RAD50)
CNY 13,712.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hRad50; NBSLD; RAD502 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110122 sequence for NM_005732 edited (data generated by NextGen Sequencing)
ATGTCCCGGATCGAAAAGATGAGCATTCTGGGCGTGCGGAGTTTTGGAATAGAGGACAAA GATAAGCAAATTATCACTTTCTTCAGCCCCCTTACAATTTTGGTTGGACCCAATGGGGCG GGAAAGACGACCATCATTGAATGTCTAAAATATATTTGTACTGGAGATTTCCCTCCTGGA ACCAAAGGAAATACATTTGTACACGATCCCAAGGTTGCTCAAGAAACAGATGTGAGAGCC CAGATTCGTCTGCAATTTCGTGATGTCAATGGAGAACTTATAGCTGTGCAAAGATCTATG GTGTGTACTCAGAAAAGCAAAAAGACAGAATTTAAAACTCTGGAAGGAGTCATTACTAGA ACAAAGCATGGTGAAAAGGTCAGTCTGAGCTCTAAGTGTGCAGAAATTGACCGAGAAATG ATCAGTTCTCTTGGGGTTTCCAAGGCTGTGCTAAATAATGTCATTTTCTGTCATCAAGAA GATTCTAATTGGCCTTTAAGTGAAGGAAAGGCTTTGAAGCAAAAGTTTGATGAGATTTTT TCAGCAACAAGATACATTAAAGCCTTAGAAACACTTCGGCAGGTACGTCAGACACAAGGT CAGAAAGTAAAAGAATATCAAATGGAACTAAAATATCTGAAGCAATATAAGGAAAAAGCT TGTGAGATTCGTGATCAGATTACAAGTAAGGAAGCCCAGTTAACATCTTCAAAGGAAATT GTCAAATCCTATGAGAATGAACTTGATCCATTGAAGAATCGTCTAAAAGAAATTGAACAT AATCTCTCTAAAATAATGAAACTTGACAATGAAATTAAAGCCTTGGATAGCCGAAAGAAG CAAATGGAGAAAGATAATAGTGAACTGGAAGAGAAAATGGAAAAGGTTTTTCAAGGGACT GATGAGCAACTAAATGACTTATATCACAATCACCAGAGAACAGTAAGGGAGAAAGAAAGG AAATTGGTAGACTGTCATCGTGAACTGGAAAAACTAAATAAAGAATCTAGGCTTCTCAAT CAGGAAAAATCAGAACTGCTTGTTGAACAGGGTCGTCTACAGCTGCAAGCAGATCGCCAT CAAGAACATATCCGAGCTAGAGATTCATTAATTCAGTCTTTGGCAACACAGCTAGAATTG GATGGCTTTGAGCGTGGACCATTCAGTGAAAGACAGATTAAAAATTTTCACAAACTTGTG AGAGAGAGACAAGAAGGGGAAGCAAAAACTGCCAACCAACTGATGAATGACTTTGCAGAA AAAGAGACTCTGAAACAAAAACAGATAGATGAGATAAGAGATAAGAAAACTGGACTGGGA AGAATAATTGAGTTAAAATCAGAAATCCTAAGTAAGAAGCAGAATGAGCTGAAAAATGTG AAGTATGAATTACAGCAGTTGGAAGGATCTTCAGACAGGATTCTTGAACTGGACCAGGAG CTCATAAAAGCTGAACGTGAGTTAAGCAAGGCTGAGAAAAACAGCAATGTAGAAACCTTA AAAATGGAAGTAATAAGTCTCCAAAATGAAAAAGCAGACTTAGACAGGACCCTGCGTAAA CTTGACCAGGAGATGGAGCAGTTAAACCATCATACAACAACACGTACCCAAATGGAGATG CTGACCAAAGACAAAGCTGACAAAGATGAACAAATCAGAAAAATAAAATCTAGGCACAGT GATGAATTAACCTCACTGTTGGGATATTTTCCCAACAAAAAACAGCTTGAAGACTGGCTA CATAGTAAATCAAAAGAAATTAATCAGACCAGGGACAGACTTGCCAAATTGAACAAGGAA CTAGCTTCATCTGAGCAGAATAAAAATCATATAAATAATGAACTAAAAAGAAAGGAAGAG CAGTTGTCCAGTTACGAAGACAAGCTGTTTGATGTTTGTGGTAGCCAGGATTTTGAAAGT GATTTAGACAGGCTTAAAGAGGAAATTGAAAAATCATCAAAACAGCGAGCCATGCTGGCT GGAGCCACAGCAGTTTACTCCCAGTTCATTACTCAGCTAACAGACGAAAACCAGTCATGT TGCCCCGTTTGTCAGAGAGTTTTTCAGACAGAGGCTGAGTTACAAGAAGTCATCAGTGAT TTGCAGTCTAAACTGCGACTTGCTCCAGATAAACTCAAGTCAACAGAATCAGAGCTAAAA AAAAAGGAAAAGCGGCGTGATGAAATGCTGGGACTTGTGCCCATGAGGCAAAGCATAATT GATTTGAAGGAGAAGGAAATACCAGAATTAAGAAACAAACTGCAGAATGTCAATAGAGAC ATACAGCGCCTAAAGAACGACATAGAAGAACAAGAAACACTCTTGGGTACAATAATGCCT GAAGAAGAAAGTGCCAAAGTATGCCTGACAGATGTTACAATTATGGAGAGGTTCCAGATG GAACTTAAAGATGTTGAAAGAAAAATTGCACAACAAGCAGCTAAGCTACAAGGAATAGAC TTAGATCGAACTGTCCAACAAGTCAACCAGGAGAAACAAGAGAAACAGCACAAGTTAGAC ACAGTTTCTAGTAAGATTGAATTGAATCGTAAGCTTATACAGGACCAGCAGGAACAGATT CAACATCTAAAAAGTACAACAAATGAGCTAAAATCTGAGAAACTTCAGATATCCACTAAT TTGCAACGTCGTCAGCAACTGGAGGAGCAGACTGTGGAATTATCCACTGAAGTTCAGTCT TTGTACAGAGAGATAAAGGATGCTAAAGAGCAGGTAAGCCCTTTGGAAACAACATTGGAA AAGTTCCAGCAAGAAAAAGAAGAATTAATCAACAAAAAAAATACAAGCAACAAAATAGCA CAGGATAAACTGAATGATATTAAAGAGAAGGTTAAAAATATTCATGGCTATATGAAAGAC ATTGAGAATTATATTCAAGATGGGAAAGACGACTATAAGAAGCAAAAAGAAACTGAACTT AATAAAGTAATAGCTCAACTAAGTGAATGCGAGAAACACAAAGAAAAGATAAATGAAGAT ATGAGACTCATGAGACAAGATATTGATACACAGAAGATACAAGAAAGGTGGCTACAAGAT AACCTTACTTTAAGAAAAAGAAATGAGGAACTAAAAGAAGTTGAAGAAGAAAGAAAACAA CATTTGAAGGAAATGGGTCAAATGCAGGTTTTGCAAATGAAAAGTGAACATCAGAAGTTG GAAGAGAACATAGACAATATAAAAAGAAATCATAATTTGGCATTAGGGCGACAGAAAGGT TATGAAGAAGAAATTATTCATTTTAAGAAAGAACTTCGAGAACCACAATTTCGGGATGCT GAGGAAAAGTATAGAGAAATGATGATTGTTATGAGGACAACAGAACTTGTGAACAAGGAT CTGGATATTTATTATAAGACTCTTGACCAAGCAATAATGAAATTTCACAGTATGAAAATG GAAGAAATCAATAAAATTATACGTGACCTGTGGCGAAGTACCTATCGTGGACAAGATATT GAATACATAGAAATACGGTCTGATGCCGATGAAAATGTATCAGCTTCTGATAAAAGGCGG AATTATAACTACCGAGTGGTGATGCTGAAGGGAGACACAGCCTTGGATATGCGAGGACGA TGCAGTGCTGGACAAAAGGTATTAGCCTCACTCATCATTCGCCTGGCCCTGGCTGAAACG TTCTGCCTCAACTGTGGCATCATTGCCTTGGATGAGCCAACAACAAATCTTGACCGAGAA AACATTGAATCTCTTGCACATGCTCTGGTTGAGATAATAAAAAGTCGCTCACAGCAGCGT AACTTCCAGCTTCTGGTAATCACTCATGATGAAGATTTTGTGGAGCTTTTAGGACGTTCT GAATATGTGGAGAAATTCTACAGGATTAAAAAGAACATCGATCAGTGCTCAGAGATTGTG AAATGCAGTGTTAGCTCCCTGGGATTCAATGTTCATTAA Clone variation with respect to NM_005732.3 >OriGene 5' read for NM_005732 unedited
GTACCATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGTCGCATTGTG GCTACGGCTTTGCGTCCCCGGCGGGCAGCCCCAGGCTGGTCCCCGCCTCCGCTCTCCCCA CCGGCGGGGAAAGCAGCTGGTGTGGGAGGAAAGGCTCCATCCCCCGCCCCCTCTCTCCCG CTGTTGGCTGGCAGGATCTTTTGGCAGTCCTGTGGCCTCGCTCCCCGCCCGGATCCTCCT GACCCTGAGATTCGCGGGTCTCACGTCCCGTGCACGCCTTGCTTCGGCCTCAGTTAAGCC TTTGTGGACTCCAGGTCCCTGGTGAGATTAGAAACGTTTGCAAACATGTCCCGGATCGAA AAGATGAGCATTCTGGGCGTGCGGAGTTTTGGAATAGAGGACAAAGATAAGCAAATTATC ACTTTCTTCAGCCCCCTTACAATTTTGGTTGGACCCAATGGGGCGGGAAAGACGACCATC ATTGAATGTCTAAAATATATTTGTACTGGAGATTTCCCTCCTGGAACCAAAGGAAATACA TTTGTACACGATCCCAAGGTTGCTCAAGAAACAGATGTGAGAGCCCAGATTCGTCTGCAA TTTCGTGATGTCAATGGAGAACTTATAGCTGTGCAAAGATCTATGGTGTGTACTCAGAAA AGCANAAAGACAGAATTTAAAACTCTGGAAGGAGTCATTACTAGAACAAAGCATGGTGAA AAGGTCAGTCTGAGCTCTAAGTGTGCAGAAATTGACCGAGNAATGATCAGTTCTCTTGGG GTTTCCCAGGCTGTNGCTAATAATGTCATTNTCTGTCATCAAGAAGAATTCTATTGGCCT TTAAGTGAAGGAAAGGGCTTTGAGCAAAGTTTGATGAGATTTTTTCAGCACAGAAACATT AAGCCTTAAAACACTNCGCAGGTACTCAGACCAGN >OriGene 3' read for NM_005732 unedited
TTGCTATGGCCCGGCGGCCGCAATCTAAGATCGCGTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTCCTTTGAAAAAAATATTTTTTTATTGACTAAGTGGATATGAAAAATAAG TTTCTTATTATACACTTTCTGAGGACCTACATTTTTATGGCATTAAAATCTTGGATATTT TTAAGGAACATTGAATCCCAGGGAGCTAACACTGCATTTAACAATCTTTGACCACTGATC GATGTTTTTTTTAATCCTGAAAAATTTCTCCACATATTCAAAACGTCCTAAAAGCTCCAC AAAATCTTCATCATGAGGGATTACCAAAAGCTGGAAGTTACGCTGCTGGGAGCGACTTTT TATTATCTCAACCAAAGCATGTGCAAAAAATTCAAGGTTTTCTCGGCCAAAATTTGTTGT GGGCTCATCCAAGGCAATGATGCCACAGTTGAGGCAAAACGTTTCAGCCAGGGCCAGGCG AATGATGAGTGAGGCTAATACCTTTTGTCCAGCACTGCATCGTCCTCGCATATCCAAGGC TGTGTCTCCCTTCAGCATCACCACTCGGTAGTTATAATTCCGCCTTTTATCAGAAGCTGA TACATTTTCATCGGCATCAGACCGTATTTCTATGTATTCAATATCTTTGCCCCGATAGGT ACTTTCGCCCCAGTTCACGTAATAATTTAATGGATTCCTTCCATTTTCATACTGGGGAAA ATTCATTATTGCCTGGGCCAAGAGCCTTAATATAAATACCAGATCCTTGTCACAAAGTTC TGGTGGCCCTCATACAACATCATTTCTTTATACTTTCCTCAGATCCGAAAATGTGTCCTT CGAAGTTTTTTCTAAAAGAAAAATTTTTCTTCACCCCTTCTGCCGCCTATGCAAATAAGG AATCTTTTTAATGGCGTATGGTCCTTCCAACTCGGAGGCCACTTTCATTGCAACCCGGCT T |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005732 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005732.2, NP_005723.2 |
| RefSeq Size | 5891 bp |
| RefSeq ORF | 3939 bp |
| Locus ID | 10111 |
| UniProt ID | Q92878 |
| Domains | Rad50_zn_hook |
| Protein Families | Druggable Genome |
| Protein Pathways | Homologous recombination, Non-homologous end-joining |
| Gene Summary | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010] Transcript Variant: This variant (1) contains an internal region absent in variant 2. The resulting protein is 139 aa longer at its N-terminus, as compared to isoform 2. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211751 | RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
CNY 13,704.00 |
|
| RC211751L1 | Lenti-ORF clone of RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
CNY 16,104.00 |
|
| RC211751L2 | Lenti-ORF clone of RAD50 (mGFP-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
CNY 15,010.00 |
|
| RC211751L3 | Lenti-ORF clone of RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
CNY 15,010.00 |
|
| RC211751L4 | Lenti-ORF clone of RAD50 (mGFP-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50) |
CNY 16,104.00 |
|
| RG211751 | RAD50 (tGFP-tagged) - Human RAD50 homolog (S. cerevisiae) (RAD50) |
CNY 15,304.00 |
