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NFS1 (NM_021100) Human Untagged Clone
CAT＃: SC110023
NFS1 (untagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 5,488.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COXPD52; HUSSY-08; IscS; NIFS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_021100, the custom clone sequence may differ by one or more nucleotides
ATGCTGCTCCGAGCCGCTTGGAGGCGGGCGGCAGTGGCGGTGACAGCGGCTCCAGGGCCGAAGCCCGCGG CGCCCACTCGGGGGCTGCGCCTGCGCGTTGGAGACCGTGCTCCTCAGTCTGCGGTTCCCGCAGATACAGC CGCTGCCCCGGAGGTGGGGCCAGTGCTGCGACCTCTCTATATGGATGTGCAAGCTACAACTCCTCTGGAC CCCCGGGTGCTTGATGCCATGCTCCCTTACCTAATCAACTACTATGGGAACCCACACTCCCGGACACATG CTTATGGCTGGGAGAGTGAGGCAGCCATGGAACGTGCTCGTCAGCAAGTAGCATCTCTGATTGGAGCTGA TCCTCGTGAGATCATTTTTACTAGTGGTGCTACTGAATCCAACAACATAGCAATTAAGGGGGTGGCCCGA TTCTACAGGTCACGGAAAAAGCACTTGATCACCACCCAGACAGAACACAAATGTGTCTTGGACTCCTGCC GTTCACTGGAAGCTGAGGGCTTTCAGGTCACCTACCTCCCAGTGCAGAAGAGTGGGATCATTGACCTAAA GGAACTAGAGGCTGCTATCCAGCCAGATACTAGCCTGGTGTCAGTCATGACTGTGAACAATGAGATTGGA GTGAAGCAGCCTATTGCAGAAATAGGGCGGATTTGCAGTTCCAGAAAGGTATATTTCCATACTGATGCAG CCCAGGCTGTTGGAAAAATCCCACTTGATGTCAATGACATGAAAATTGATCTCATGAGCATTAGTGGTCA CAAAATCTACGGTCCCAAAGGGGTTGGTGCCATCTACATCCGTCGCCGGCCCCGTGTGCGTGTGGAGGCC CTGCAGAGTGGAGGGGGGCAGGAGCGGGGTATGCGGTCTGGGACAGTGCCCACACCCTTAGTGGTGGGGC TGGGGGCTGCGTGTGAGGTGGCACAGCAAGAGATGGAGTATGACCACAAGCGAATCTCAAAGTTGTCAGA GCGGCTGATACAGAATATAATGAAGAGCCTTCCAGATGTGGTGATGAATGGGGACCCTAAGCACCATTAT CCCGGCTGTATCAACCTCTCCTTTGCATATGTGGAAGGGGAAAGTCTGCTGATGGCACTGAAGGACGTTG CCTTATCCTCAGGGAGTGCCTGCACCTCTGCATCCCTGGAGCCCTCTTATGTGCTTAGAGCAATTGGCAC TGATGAGGATTTAGCGCACTCTTCTATCAGGTTTGGAATTGGCCGCTTCACTACAGAGGAGGAAGTGGAC TACACAGTGGAGAAATGCATTCAGCATGTGAAGCGTCTTCGAGAAATGAGCCCTCTCTGGGAGATGGTTC AGGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAACACTAG >OriGene 5' read for NM_021100 unedited
GTCATATTTGTATACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGTGGGCTCTGC CAGCGGGACCATGCTGCTCCGAGCCGCTTGGAGGCGGGCGGCAGTGGCGGTGACAGCGGC TCCAGGGCCGAAGCCCGCGGCGCCCACTCGGGGGCTGCGCCTGCGCGTTGGAGACCGTGC TCCTCAGTCTGCGGTTCCCGCAGATACAGCCGCTGCCCCGGAGGTGGGGCCAGTGCTGCG ACCTCTCTATATGGATGTGCAAGCTACAACTCCTCTGGACCCCCGGGTGCTTGATGCCAT GCTCCCTTACCTAATCAACTACTATGGGAACCCACACTCCCGGACACATGCTTATGGCTG GGAGAGTGAGGCAGCCATGGAACGTGCTCGTCAGCAAGTAGCATCTCTGATTGGAGCTGA TCCTCGTGAGATCATTTTTACTAGTGGTGCTACTGAATCCAACAACATAGCAATTAAGGG GGTGGCCCGATTCTACAGGTCACGGAAAAAGCACTTGATCACCACCCAGACAGAACACAA ATGTGTCTTGGACTCCTGCCGTTCACTGGAAGCTGAGGGCTTTCAGGTCACCTACCTCCC AGTGCAGAAGAGTGGGATCATTGACCTAAAGGAACTAGAGGCTGCTATCCAGCCAGATAC TAGCCTGGTGTCAGTCATGACTGTGAACAATGAGATTGGAGTGAAGCAGCCTATTGCAGA AATAGGGCGGATTTGCAGTTCCAGAAAGGTATATTTCCATACTGATGCAGCCCAGGCTGT TGGAAAAATCCACTTGATGTCAATGACATGAAAATTGATCTCATGAGCATAAGTGGTCAC CAAATCTACGGTCCCCAAGGGGTTTGGTGCCATCTACATNCGTCGCCGCCCCCGTGTGCG TTGTGGAGCCTTGCACAGTGAAC >OriGene 3' read for NM_021100 unedited
CGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAAATAAAAGGTGAGTTTATT TCTCCAGTCTTGAAATTAACAATTTTCAAGAAAACAAATTATTAGAATAACTGGTGGAGT CACAGACATATTTCTAGCTCCATTAGGTCAAAGGAAAGGAAAGAGGACAAGGGTAGAAAG GAGACAAGCCCTCTAAGAAACTGTCCATTCAGTCTGTCTGCAGTCAATATGAAGAGATAG CCTTTGGAGCTACAGAAAATATTACATTGAAGTGGATTATGCTTGCTGTTAAAGAAGCTG GTCCAGCCGAGCAGCAGCAGAAGACGAAGACTCAAATGTCCATGTAGAGCATCATGGTCC CTGACCAAAGAGACTTCTTCTGGTTCCTCTTGCTCTCCTCACCAAGAGCACAGATGTGCC CAGCAGCAATATGACAGCAAATGTCTCTATGGCTTCGGGATGAAAATGTCCACACACTTT AAGACCCGAGAAGAAATGGGGAGTGCTCCACACCCAAGGAACTGAAGCTAGGGAAAGACA GTTTTCTTGTGTTTCTGTCATAGAGGTGGACTGATGCTGAAGTCAACTGGTCTATACCAA TCTAGAGCATCCACTAGGTGTAACAAGGTGTCTGGTTGTGCACGGGTTGGTGAGGCAGGA GGGGCCANACCAGCACAAAGTCAGGGCCCTATTCTTCTAGTGTTGGGTCCACTTGATGCT CTAGAGGTCAATGCCATCCTGAACCATCTCCCGAGAGGGCTCATTTCTCGAAGACGCTTT CACATGCTGATGCATTCCTCCACTGGGTAGTCCACTTCCTCCTCTGTAGTGAAGCGGGCA ATTCCAACCTGATAGAAGATGCGCTAAATCTCATAGTGGCCATTGCTCTAGCCCATAGAG GCCTCAGGATGCANAGTGCGAGCACNCCTGAGATAAGGCACCGNCCTCATGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021100 |
| Insert Size | 2440 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021100.3, NP_066923.3 |
| RefSeq Size | 2371 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 9054 |
| UniProt ID | Q9Y697 |
| Domains | aminotran_5 |
| Protein Pathways | Thiamine metabolism |
| Gene Summary | Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (1) encodes the longer isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209405 | NFS1 (Myc-DDK-tagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,488.00 |
|
| RC209405L1 | Lenti ORF clone of Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC209405L2 | Lenti ORF clone of Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209405L3 | Lenti ORF clone of Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209405L4 | Lenti ORF clone of Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG209405 | NFS1 (tGFP-tagged) - Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 7,088.00 |
|
| SC322710 | NFS1 (untagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,488.00 |
