CDK10 (NM_003674) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDC2-related protein kinase; cell division protein kinase 10; cyclin-dependent kinase (CDC2-like) 10; cyclin-dependent kinase 10; cyclin-dependent kinase related protein; PISSLRE; serine/threonine protein kinase PISSLRE
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_003674 edited TTCGGCACGAGGGCGGAGCCAGATCTGGAGTGCGAGCAGATCCGTCTGAAGTGTATTCCT GGGACGATGCCGGAGTGTGAAGGAGTTTGAGAAGCTGAACCGCATTGGAGAGGGTACCTA CGGCATTGTGTATCGGGCCCGGGACACCCAGACAGATGAGATTGTCGCACTGAAGAAGGT GCGGATGGACAAGGAGAAGGATGGCATCCCCATCAGCAGCTTGCGGGAGATCACGCTGCT GCTCCGCCTGCGTCATCCGAACATCGTGGAGCTGAAGGAGGTGGTTGTGGGGAACCACCT GGAGAGCATCTTCCTGGTGATGGGTTACTGTGAGCAGGACCTGGCCAGCCTCCTGGAGAA TATGCCAACACCCTTCTCGGAGGCTCAGGTCAAGTGCATCGTGCTGCAGGTGCTCCGGGG CCTCCAGTATCTGCACAGGAACTTCATTATCCACAGGGACCTGAAGGTTTCCAACTTGCT CATGACCGACAAGGGTTGTGTGAAGACAGGTGGGTGCAACTTGGGCCAGGCCCTGTCCCT AGATGGCACTTGGTGACACACACTCCCCTCTCTGCTGCAGCGGATTTCGGCCTGGCCCGG GCCTATGGTGTCCCAGTAAAGCCAATGACCCCCAAGGTGGTCACTCTCTGGTACCGAGCC CCTGAACTGCTGTTGGGAACCACCACGCAGACCACCAGCATCGACATGTGGGCTGTGGGC TGCATACTGGCCGAGCTGCTGGCGCACAGGCCTCTTCTCCCCGGCACTTCCGAGATCCAC CAGATCGACTTGATCGTGCAGCTGCTGGGCACGCCCAGTGAGAACATCTGGCCGGGCTTT TCCAAGCTGCCACTGGTCGGCCAGTACAGCCTCCGGAAGCAGCCCTACAACAACCTGAAG CACAAGTTCCCATGGCTGTCGGAGGCCGGGCTGCGCCTGCTGCACTTCCTGTTCATGTAC GACCCTAAGAAAAGGGCGACGGCCGGGGACTGCCTGGAGAGCTCCTATTTCAAGGAGAAG CCCCTACCCTGTGAGCCGGAGCTCATGCCGACCTTTCCCCACCACCGCAACAAGCGGGCC GCCCCAGCCACCTCCGAGGGCCAGAGCAAGCGCTGTAAACCCTGACGGTGGGCCTGGCAC ACGCCTGTATTCCCACACCAGGTCTTCCGATCAGTGGTGTCTGTGAAGGGTGCCGCGAGC CAGGCTGACCAGGCGCCCGGGATCCAGCTCATCCCCTTGGCTGGGAACATCCTCCACTGA CTTCCTCCCACTGTCTGCCCTGAACCCACTGCTGCCCCCAGAAAAAGGCCGGGTGACACC GGGGGGCTCCCAGCCCGTGCACCCTGGAAGGGCAGGTCTGGCGGCTCCATCCGTGGCTGC AGGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_003674 unedited CGGGGGCACCCCCTTTTTCCCCCCCCGGTTTAGATTTGTATACAATCTTATAGGCGGCCG CGNAATTCGCACGAGGCGNGACCAGATCTGGAGTGCGAGCAGATCCGTCTGAAGTGTATT CCTGGGACGATGCCGGAGTGTGAAGGAGTTTGAGAAGCTGAACCGCATTGGAGAGGGTAC CTACGGCATTGTGTATCGGGCCCGGGACACCCAGACAGATGAGATTGTCGCACTGAAGAA GGTGCGGATGGACAAGGAGAAGGATGGCATCCCCATCAGCAGCTTGCGGGAGATCACGCT GCTGCTCCGCCTGCGTCATCCGAACATCGTGGAGCTGAAGGAGGTGGTTGTGGGGAACCA CCTGGAGAGCATCTTCCTGGTGATGGGTTACTGTGAGCAGGACCTGGCCAGCCTCCTGGA GAATATGCCAACACCCTTCTCGGAGGCTCAGGTCAAGTGCATCGTGCTGCAGGTGCTCCG GGGCCTCCAGTATCTGCACAGGAACTTCATTATCCACAGGGACCTGAAGGTTTCCAACTT GCTCATGNACCGACAGGGTTGTGTGAAGACAGGTGGGTGCAACTTGNGCCAGGCCCTGTC CCTAGATGGCACTTGGTGACACACACTCCCCTCTCTGCTGCAGCGGATTTCGGCCTGGCC CGGGCCTATGGTGTCCCCAGTAAGCCAATGACCCCCAGGTGGTCACTCTCTGGTACCGAG CCCCTGAACTGCTNNGTGGAACCACCCGCANACACCAGCATCGACATGTTGGCTGTGGNC TGCATACTGGGCGAGCTTGCTGCGCACAAGCCTTCTCTTCCCGGCACTTTCGANATCACC ANATCGACTTGATCGTGCAGCTGCTGGGCCCGCCCAGTGAAAACATCTTGGCGGGGCTTT TCAAGCTGNCACTGGTCCGCCATTACAGCTTCGGGAGCAGCCCTACACCAACCTGAGCAC AGTTCCCATGGCTGTTGAAGGCGGGCTTGNCCTGNTGACATTCTTGTNATGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_003674 unedited CCGCGCCCCAATTGCGGGTNCAAACCCCCAGTTCCAAGATCTTCGCTCAGCATCAAAGCC ACGCCTCCTGTTGGGTCCTCCAGTGGGGAGCAGGGATGCAGCTCAGCCTCCAGCCCAGGG CTCTCCTGGTAGTCTCGGGGTATCCTCAATTGTGGCTCCCACCCCATACCTTGTCCCTCT TGTCCTCATATGGAGTCCTGCCAGGCCCACCTGGGGTCCCCACAGCCAACCCATGCACGG GACAGCCCCGGGCGACTGAGAGGGGATACGAAGGTCTTCTGGGCGCTCTCATCCCAGGCC AGCGTCCACCACAACTCAAAGACAGCGGGGGGACTGCACCCACCACTCCTCCCAAGAAGC AGTGGTTGCACATTTCCAACATAGCGAGGAACACCACATGATACCCCTGCAGCCACGGAT GGAGCCGCCAGACCTGCCCTTCCAGGGTGCACGGGCTGGGAGCCCCCCGGTGTCACCCGG CCTTTTTCTGGGGGCAGCAGTGAATTCAGGGCAGACAGTGGGAGGAAGTCAGTGGAGGAT GTTCCCAGCCAAGGGGATGAGCTGGATCCCGGGCGCCTGGACAGCCTGGCTATCGGCACC CTTCACAGACACCACTGATCGGAAGACCTGGTGTGGGAATACATGCGTGTGCCAGGCCCA CCGTCAGGGTTTACAGCGCTTGCTCTGGCCCTCGGAGGATGCTGGGGCGGCCCGCTTGAT GCGGTGGTGGGGAAAGGTCGGCATGAGCTCCGGCTCACAGGGTAGGGGCTTCTCAGTGAA ATAGGAGCTCTCCAGGCAGTCCCGGCCGTCGCCCTTTCTTAGGGTCGTACATGAACAGGA GTTGCACAGGCGCACCCGGCCTCCACAGCCAGGGAAACTGGGCTTCAGT
Restriction Sites	NotI-NotI
ACCN	NM_003674
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003674.2, NP_003665.2
RefSeq Size	1913 bp
RefSeq ORF	996 bp
Locus ID	8558
Domains	pkinase, TyrKc, S_TKc
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	The protein encoded by this gene belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation and its function is limited to cell cycle G2-M phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] Transcript Variant: This variant (1) encodes the longest isoform (1). It initiates translation at a non-AUG (CUG) start site and contains a putative, alternative in frame non-AUG (GUG) start codon.

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