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NEK3 (NM_002498) Human Untagged Clone
CAT＃: SC109481
NEK3 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 1
CNY 3,776.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSPK36 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002498, the custom clone sequence may differ by one or more nucleotides
ATGGATGACTACATGGTCCTGAGAATGATTGGGGAGGGCTCCTTCGGCAGAGCTCTTTTGGTTCAGCATG AAAGCAGTAATCAGATGTTTGCCATGAAAGAAATAAGGCTTCCCAAGTCTTTCTCTAATACACAGAATTC TAGGAAGGAGGCTGTTCTTTTAGCCAAAATGAAACACCCTAATATTGTTGCCTTCAAAGAATCATTTGAA GCTGAAGGACACTTGTATATTGTGATGGAATACTGTGATGGAGGGGATCTAATGCAAAAGATTAAACAGC AGAAAGGAAAGTTATTTCCTGAAGACATGATACTTAATTGGTTTACCCAAATGTGCCTTGGAGTAAATCA CATTCACAAGAAACGTGTGCTACACAGAGATATCAAGTCCAAGAATATCTTCCTCACTCAGAATGGAAAA GTGAAATTGGGAGACTTTGGATCTGCCCGTCTTCTCTCCAATCCGATGGCATTTGCTTGTACCTATGTGG GAACTCCTTATTATGTGCCTCCAGAAATTTGGGAAAACCTGCCTTATAACAATAAAAGTGACATCTGGTC CTTGGGTTGCATCCTGTATGAACTCTGTACCCTTAAGCATCCATTTCAGGCAAATAGTTGGAAAAATCTT ATCCTCAAAGTATGTCAAGGGTGCATCAGTCCACTGCCGTCTCATTACTCCTATGAACTTCAGTTCCTAG TCAAGCAGATGTTTAAAAGGAATCCCTCACATCGCCCCTCGGCTACAACGCTTCTCTCTCGAGGCATCGT AGCTCGGCTTGTCCAGAAGTGCTTACCCCCCGAGATCATCATGGAATATGGTGAGGAAGTATTAGAAGAA ATAAAAAATTCGAAGCATAACACACCAAGAAAAAAAACAAACCCCAGCAGAATCAGGATAGCTTTGGGAA ATGAAGCAAGCACAGTGCAAGAGGAAGAACAAGATAGAAAGGGTAGCCATACTGATTTGGAAAGCATTAA TGAAAATTTAGTTGAAAGTGCATTGAGAAGAGTAAACAGAGAAGAAAAAGGTAATAAGTCAGTCCATCTG AGGAAAGCCAGTTCACCAAATCTTCATAGACGACAGTGGGAGAAAAATGTACCCAATACAGCTCTTACAG CTTTGGAAAATGCATCCATACTCACCTCCAGTTTAACAGCAGAGGACGATAGAGGTGGTTCTGTAATAAA GTACAGCAAAAATACTACTCGTAAGCAGTGGCTCAAAGAGACCCCTGACACTTTGTTGAACATCCTTAAG AATGCTGATCTCAGCTTGGCTTTTCAAACATACACAATATATAGACCAGGTTCAGAAGGGTTCTTGAAAG GCCCCCTGTCTGAAGAAACAGAAGCATCGGACAGTGTTGATGGAGGTCACGATTCTGTCATTTTGGATCC AGAGCGACTTGAGCCTGGGCTAGATGAGGAGGACACGGACTTTGAGGAGGAAGATGACAACCCCGACTGG GTGTCAGAGCTGAAGAAGCGAGCTGGATGGCAAGGCCTGTGCGACAGATAA >OriGene 5' read for NM_002498 unedited
NGGGTATAACACCCGCCCGTTGGCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT ATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGG CCGCGAATTCGGCACGAGGATAAGGGTTATTCAGTAAAGTTTGTTATGTGGACTCAAGTT GGTGCCTTCCCCATTGGTAAGAGTTGCTCTCCTCTTCCTGGTACTGGAAAGGAAGACACA TTTACAAATGGATATTTATTACCTGTACATAGGAAAATTTATGCCCTGCATTTATACAAA AAAGGGAGGGCAAAGAGCAAATTGGTTTCAGCTCAAAACAATTCTTACATGAAAGTGGCA TATTTTGGGGTAGCATACACTGATCCTCTTCAAAAGGAATAATTTGCTTTTCTCTTATCA TGGTTATGAGAGCTACTTTGAATTAATTTTTGCTTTTTGAAAGTACATTTAATTCTCAAA TGTTTCTGCTTTTGAATGCAGTTTGGGAGAGCCCATGGTGACTGCGTGAGTGGAGCCCAG CTGTGTGGATGCCCCAGCATGGATGACTACATGGTCCTGAGAATGATTGGGGAGGGCTCC TTCGGCAGAGCTCTTTTGGTTCAGCATGAAAGCAGTAATCAGATGTTTGCCATGAAAGAA ATAAGGCTTCCCAAGGTCACTACTAAAGAAATTAGCTCACTTTGAAAGTATACATGCAGT TTATATAAAAGAATAAGTTTTTGGCCAGGCACAGTGGCTCACACCTGTAATCCCAGCACT TTGGGAGGTGGGAGGCGGGTAGATCACCTGAGGTCAGGAGTTTGAGACCGGCCTGAGGTC ANGAGTTTGAGGCCAGCCTGGCCAACATGGGGAAAACCCGTCTCTN >OriGene 3' read for NM_002498 unedited
AACTGTGTACCGCGCCGCATTCTAGGATCGAGTTAAATTCTTTTTTTTTTTTTTTCATAA TTAAATATATTTAATTCTCCAAACCTTTAGGAAATGTAAAAAGAGTCACATAATAATAAA CACATATCATAATGTGAATAATATCATACGGGCTGCTATAAAGGTCTGTGGTCAGCATCC CTCTGAGGTAGGCATTATTATCTTCATTTTATGGTTTAGGAAATTGGGGCTTGAAGAGGT TAAGTAATTTCCCACAATCACACAAGTAGGTAGTGGCACGCTAGCATCCCATTTCTGTTC TATGGGACTGCAAAGCCCGATGCTCACACTCATTCCACTATACCTTCTCCCTTGAGTTCA AAAACTTACAGGAGTTCTAGACTTTTCAAACTCACGATACTAATCAAGAACGATTTTAAG TATTAAGAGTTTCCTCTGCTTCATTCTGTTTCCAAAGGAAAATATACGTCGCATGAACTC ATGATCATCTCAGCATGAACTCCTGAGTGAAGCCTCTCCTCAGCGTGACTCAGGAACATT TCCTCAGGCATTATCTGTCGCACAGGCCTTGCCATCCAGCTCGCTTCTTCAGCTCTGACA CCCAGTCGGGGTTGTCATCTTCCTCCTCAAATCCGTGTCCTCCTCATCTAGCCCAGGCTC AAGTCGCTCTGGATCCAAAATGACAGAATCGTGACCTCCATCAACACTGTCCGATGCCTC TGTTTCTTCAGACAGGGGCCTTTCAAGAACCTTCTGAACCGGGTCATAATTGTGTAGTTT GAAAACCCAACTGAATCACCATTCTTAGGATGTCAACAAGTGTCAGGGGTCTCTTGAGCA CTGCTTACAGTAGAATTTTTGCCGGACTTAATACAGAACCACCTCTTCGTTCTTGGCTTT AAACGGAAGTGAATTTGGATGCATTTCCAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002498 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002498.1, NP_002489.1 |
| RefSeq Size | 2332 bp |
| RefSeq ORF | 1521 bp |
| Locus ID | 4752 |
| UniProt ID | P51956 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Two functional alleles for this gene have been identified in humans. The reference genome assembly (GRCh38) represents a functional allele that is associated with the inclusion of an additional coding exon in protein-coding transcripts, compared to an alternate functional allele that lacks the exon. [provided by RefSeq, Sep 2019] Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (a). Sequence Note: This sequence contains an extra 'A' at the end of exon 10 (nt 1253) that is not found in the reference (NC_000013.9), Celera, or HuRef assemblies. It represents the product of an alternate haplotype supported by human transcripts and conserved with other primate genomes. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217714 | NEK3 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 1 |
CNY 3,768.00 |
|
| RC217714L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217714L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217714 | NEK3 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 1 |
CNY 4,370.00 |
