MID1 (NM_033291) Human Untagged Clone

CAT#: SC109439

MID1 (untagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 2



CNY 4,120.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-TRI18/MID1 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms OS, FXY, OSX, OGS1, XPRF, BBBG1, GBBB1, RNF59, ZNFXY, TRIM18
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_033291, the custom clone sequence may differ by one or more nucleotides


ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCTCTTCTACTGC
CCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCACACTGTGCCACCAACGAGTC
TGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTA
GACGGGCTCAAGCGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGC
CCAACTCTCCCAGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGT
CCTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCC
TACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC
CAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTG
TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA
GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGA
ACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACG
TCAAGAAGCCAAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAGATTATT
GGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAGATTGCAAACTGCAAAC
AGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACACTCTCTGAAGGAGAATGATCATGCGCG
TTTCCTACAGACTGCTAAGAATATCACCGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTTCTAATT
CCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAG
AATGTCTGGATTACCTTACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGA
CACCATCACTGTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA
TTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATGATAGTACCCAACATCA
AGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAGTACATCTTCATGGTCAAGGCCATCAA
CCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAGTTGAAGACAAACAGCCAACCATTTAAACTGGATCCC
AAATCTGCTCATCGAAAACTGAAGGTGTCCCATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCA
AGAAGAGTCACACACCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGA
TAGTGGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTGA


>OriGene 5' read for NM_033291 unedited
NCCTGTCGGATTTTGTATACGACTCCTATAGGCGGCNCGCGAATTCGCACGAGGCTCGAG
CAGAGTGCGTGTAGCAACAGATCAAAGAAAAAGAGACGAAAACGTGCTCTTTGCTGCCCG
TAGATTTCGCCGGGTTGCTTTTGTCTTGCGGGCTCCTGTCGGGTTCGGTGTTTCCGCTCT
GAAGACTGCGACGCGGGCTCCGATGCAGCTCGCTCCCTGCCGGATGGGTCATGGGATTCT
AAACATGAGGCAGATAGCTGATCAGCTTCCTTGGGTTTTGCTGATGACACAAGAGAGCTT
TGCCTGAAGATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTT
GAGGACCCTCTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATC
CTAGTATCACACTGTGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTCCAGTGCCCC
ACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAGCGCAACGTC
ACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCCAACTCTCCC
AGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGTC
CTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGT
GAAGTATCCTACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACA
GGCCATCGTCTGATTGAGCCAATTCCGGACTCTCACATNNCGNNGGCTGATGTGCTTGGA
GCATGANNGATGAGAAGTGGAAATATGTACTGTGTGACCGATGACCAGTTAATCTGTGCC
CTGTGTAAACTGGNNTGGCGNCACCGCGATCATCAGG
>OriGene 3' read for NM_033291 unedited
ACTATGAACCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTGGTCTTA
CAACTCTATTGTAAACTATACTAGACTATAGAGGGACTTCTACATCTTTCAAGATGTGTT
TAATAAAGGTCTGTTTATAATAACTTTTGAGGCATGAATCTAGCAAATAGTACTTTATAC
AATGTCCCTTGTCATTACCAACTCATAAATATTAAGTGTTTTTCAGTGACTTATGTTTGG
ATGTGGTAGTGCTGATCAGGGCCATGTGCTGATGTCCTGGAGAGCAAAATCAATCCAAAG
TGGTGCTGCTATTTGTGACAGAACATGTTTATTTACTCAGCCCCGGAGACAAAAGGAAAA
TTGATATGGGGGAGCGGGAAATAGGAGAACTATTAAATGTAGTGAAGAAATTTCACAGGT
CTAAAGGAACTATTAAAAGGAAGGATAAAGTAGATTCTATACTATAAAACAGAATCCTAC
CTCTGATAAAAGACAAATCAGCCTGAATTTTTGAATAATCAATAGGATTCAAAATGACTA
TTTTCAATTGCAATCTCATTCTTAATGTTCTTCCAGAACCCTTAACCCTGAGAGAAGCAG
GGCAAAATGTGGCCAAAGGATTCAGAACCTTTCTTTCCAACATTATCCCATCAGCTATGA
GAAGGTAAAGATTTAGCCACGGCAAGGTCCGAGGTCCAAAATCAACTCTTTTCATTCTCT
GTCTTGCTCTCCATTTTAGTTGATCTGATACAACTTTTTGACATCACATTAAAACACTTC
AATTATACCCNAGTAAGAAGACTGTTTTCTNCCCTACTAAGTTCAACACATCCTGTTATG
CCCACTCGAGTGAGAAGACTACCCTGGGTCATCATGGGTATCAGGTAGAGATGAGTACAT
CCAACTCCAAATTTTGTTTTTTGTAGCCCCGATGTGATAAATAAAGTGTAATA
Restriction Sites NotI-NotI     
ACCN NM_033291
Insert Size 3600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_033291.1, NP_150633.1
RefSeq Size 3352 bp
RefSeq ORF 1659 bp
Locus ID 4281
Domains zf-B_box, RING, BBC, FN3
Protein Families Druggable Genome
Protein Pathways Ubiquitin mediated proteolysis
Gene Summary The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]
Transcript Variant: Variant 2 (beta) uses an alternate splice site near the end of the coding region, introducing a frameshift and an earlier stop codon as compared to variant 1. The beta isoform is thus 115 aa shorter than isoform alpha.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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