MCM7 (NM_182776) Human Untagged Clone
CAT#: SC109412
MCM7 (untagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2
CNY 4,048.00
CNY 7,220.00
Product images
CNY 6,281.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_182776, the custom clone sequence may differ by one or more nucleotides
ATGGTGGTGGCCACTTACACTTGTGACCAGTGTGGGGCAGAGACCTACCAGCCGATCCAGTCTCCCACTT TCATGCCTCTGATCATGTGCCCAAGCCAGGAGTGCCAAACCAACCGCTCAGGAGGGCGGCTGTATCTGCA GACACGGGGCTCCAGATTCATCAAATTCCAGGAGATGAAGATGCAAGAACATAGTGATCAGGTGCCTGTG GGAAATATCCCTCGTAGTATCACGGTGCTGGTAGAAGGAGAGAACACAAGGATTGCCCAGCCTGGAGACC ACGTCAGCGTCACTGGTATTTTCTTGCCAATCCTGCGCACTGGGTTCCGACAGGTGGTACAGGGTTTACT CTCAGAAACCTACCTGGAAGCCCATCGGATTGTGAAGATGAACAAGAGTGAGGATGATGAGTCTGGGGCT GGAGAGCTCACCAGGGAGGAGCTGAGGCAAATTGCAGAGGAGGATTTCTACGAAAAGCTGGCAGCTTCAA TCGCCCCAGAAATATACGGGCATGAAGATGTGAAGAAGGCACTGCTGCTCCTGCTAGTCGGGGGTGTGGA CCAGTCTCCTCGAGGCATGAAAATCCGGGGCAACATCAACATCTGTCTGATGGGGGATCCTGGTGTGGCC AAGTCTCAGCTCCTGTCATACATTGATCGACTGGCGCCTCGCAGCCAGTACACAACAGGCCGGGGCTCCT CAGGAGTGGGGCTTACGGCAGCTGTGCTGAGAGACTCCGTGAGTGGAGAACTGACCTTAGAGGGTGGGGC CCTGGTGCTGGCTGACCAGGGTGTGTGCTGCATTGATGAGTTCGACAAGATGGCTGAGGCCGACCGCACA GCCATCCACGAGGTCATGGAGCAGCAGACCATCTCCATTGCCAAGGCCGGCATTCTCACCACACTCAATG CCCGCTGCTCCATCCTGGCTGCCGCCAACCCTGCCTACGGGCGCTACAACCCTCGCCGCAGCCTGGAGCA GAACATACAGCTACCTGCTGCACTGCTCTCCCGGTTTGACCTCCTCTGGCTGATTCAGGACCGGCCCGAC CGAGACAATGACCTACGGTTGGCCCAGCACATCACCTATGTGCACCAGCACAGCCGGCAGCCCCCCTCCC AGTTTGAACCTCTGGACATGAAGCTCATGAGGCGTTACATAGCCATGTGCCGCGAGAAGCAGCCCATGGT GCCAGAGTCTCTGGCTGACTACATCACAGCAGCATACGTGGAGATGAGGCGAGAGGCTTGGGCTAGTAAG GATGCCACCTATACTTCTGCCCGGACCCTGCTGGCTATCCTGCGCCTTTCCACTGCTCTGGCACGTCTGA GAATGGTGGATGTGGTGGAGAAAGAAGATGTGAATGAAGCCATCAGGCTAATGGAGATGTCAAAGGACTC TCTTCTAGGAGACAAGGGGCAGACAGCTAGGACTCAGAGACCAGCAGATGTGATATTTGCCACCGTCCGT GAACTGGTCTCAGGGGGCCGAAGTGTCCGGTTCTCTGAGGCAGAGCAGCGCTGTGTATCTCGTGGCTTCA CACCCGCCCAGTTCCAGGCGGCTCTGGATGAATATGAGGAGCTCAATGTCTGGCAGGTCAATGCTTCCCG GACACGGATCACTTTTGTCTGA >OriGene 5' read for NM_182776 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGAGGTCCCGGTGAAAT CAGGGGCTAAGGGGACCCAAAGAAGGCGGGGGATCATAGGGGTGGAAAGAAAGCTGAGAA CCTTGAGACCGGAGTGTGAGGGGCCAACGGGGAAGGGCGCTAGAATTTTAAACTAAAGTA GGGACCGGAATTCCCCTGGGGAGATGTTGGATGGCCCTGTGCACTGCCACGGGCTCTTTA TTCTTCGCTGGTTAGAAACAGACTTGTGAAAAAGAGTTATGCCCACTTTGGGGAGACTTC GAAAAGGTTAAGAAGTTCTTACAAGAGTTCTACCAGGATGATGAACTCGGGAAGAAGCAG TTCAAGTATGGGAACCAGTTGGTTCGGCTGGCTCATCGGGAACAGGTGGCTCTGTATGTG GACCTGGACGACGTAGCCGAGGATGACCCCGAGTTGGTGGACTCAATTTGTGAGAATGCC AGGCGCTACGCGAAGCTCTTTGCTGATGCCGTACAAGAGCTGCTGCCTCAGTACAAGGAG AGGGAAGTGGTAAATAAAGATGTCCTGGACGTTTACATTGAGCATCGGCTAATGATGGAG CAGCGGAGTCGGGACCCTGGGATGGTCCGAAGCCCCCAGAACCAGTACCCTGCTGAACTC ATGCGCAGATTGTGAGTGGTCTCTGTCGGGAAAGATGTANGGATTGGTTCTCCAGGATCT TGGTTGTGACTGTTNTCTCCCCTTAGTGAGCTGTATTTTCAGGCCCTAGCAGCAGCAAGC CTCGTGTGATCCGGGAAGTGCGGGCTGACTCTGGGGGGAAGTGGTAACTGTGCGTGAATC GTACTCGTGCTCTGAGTCAAACCCAGAGGTGGTGGCACTACACTGTGACACGGTGGGAGA GACTACAGNCGATCAGCTNCCATTTCATGCCTGT |
Restriction Sites | NotI-NotI |
ACCN | NM_182776 |
Insert Size | 2800 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_182776.1, NP_877577.1 |
RefSeq Size | 2900 bp |
RefSeq ORF | 1632 bp |
Locus ID | 4176 |
UniProt ID | P33993 |
Protein Families | Transcription Factors |
Protein Pathways | Cell cycle, DNA replication |
Gene Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs at the 5' end, contains an additional internal segment, and initiates translation from an in-frame downstream AUG compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203630 | MCM7 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2 |
CNY 5,272.00 |
|
RC203630L3 | Lenti ORF clone of Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203630L4 | Lenti ORF clone of Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG203630 | MCM7 (tGFP-tagged) - Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2 |
CNY 4,370.00 |