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NPL (NM_030769) Human Untagged Clone
CAT＃: SC109402
NPL (untagged)-Human N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 1
CN¥ 2,400.00
CN¥ 6,270.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C1orf13; C112; NAL; NPL1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC109402 sequence for NM_030769 edited (data generated by NextGen Sequencing)
ATGGCCTTCCCAAAGAAGAAACTTCAGGGTCTTGTGGCTGCAACCATCACGCCAATGACT GAGAATGGAGAAATCAACTTTTCAGTAATTGGTCAGTATGTGGATTATCTTGTGAAAGAA CAGGGAGTGAAGAACATTTTTGTGAATGGCACAACAGGAGAAGGCCTGTCCCTGAGCGTC TCAGAGCGTCGCCAGGTTGCAGAGGAGTGGGTGACAAAAGGGAAGGACAAGCTGGATCAG GTGATAATTCACGTAGGAGCACTGAGCTTGAAGGAGTCACAGGAACTGGCCCAACATGCA GCAGAAATAGGAGCTGATGGCATCGCTGTCATTGCACCGTTCTTCCTCAAGCCATGGACC AAAGATATCCTGATTAATTTCCTAAAGGAAGTGGCTGCTGCCGCCCCTGCCCTGCCATTT TATTACTATCACATTCCTGCCTTGACAGGGGTAAAGATTCGTGCTGAGGAGTTGTTGGAT GGGATTCTGGATAAGATCCCCACCTTCCAAGGGCTGAAATTCAGTGATACAGATCTCTTA GACTTCGGGCAATGTGTTGATCAGAATCGCCAGCAACAGTTTGCTTTCCTTTTTGGGGTG GATGAGCAACTGTTGAGTGCTCTGGTGATGGGAGCAACTGGAGCAGTGGGCAGTACCTAT AACTACCTGGGAAAAAAGACAAACCAGATGTTGGAGGCTTTTGAACAAAAGGACTTCTCT TTAGCCCTGAACTATCAGTTTTGTATCCAGAGATTTATCAACTTTGTTGTCAAACTAGGT TTTGGAGTGTCACAGACCAAAGCCATCATGACTCTGGTCTCTGGGATTCCAATGGGCCCA CCCCGGCTTCCACTGCAGAAAGCCTCCAGGGAGTTTACTGATAGTGCTGAAGCTAAACTG AAGAGCCTGGATTTCCTTTCTTTCACTGATTTAAAGGATGGAAACTTGGAAGCTGGTAGC TAG Clone variation with respect to NM_030769.2 >OriGene 5' read for NM_030769 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAAGAGCGGAGGCCTGGG TGAGCGCGGCCCGCGACGGCACGGGCTAGGACAGACGGACGCAGACGGCCCGACGACCTG GGCGTTCGGGCGGAGGGGCGCTGGGGCCTGACTCGCGGAAGGGGAAACTGAAGCAGAAAG AGGTTACTTAATGGGCTCAAGGTCACCTACCTGACCTACCAGCAGCTCAATGGCCTTCCC AAAGAAGAAACTTCAGGGTCTTGTGGCTGCAACCATCACGCCAATGACTGAGAATGGAGA AATCAACTTTTCAGTAATTGGTCAGTATGTGGATTATCTTGTGAAAGAACAGGGAGTGAA GAACATTTTTGTGAATGGCACAACAGGAGAAGGCCTGTCCCTGAGCGTCTCAGAGCGTCG CCAGGTTGCAGAGGAGTGGGTGACAAAAGGGAACGACAAGCTGGATCAGGTGATAATTCA CGTAGGAGCACTGAGCTTGAACGAGCTACAGGAACTGGCCCAACATGCACCATAAATACG AGCTGATGGCATCGCTGTCATTGCACCGTTCTTNCTCCAGCCATGGACCAAAGATATCCT GCATAATTCCCTAAAGGAACCGCCTGCTGCCGCCCCTTAACTGCCTTTATATTATTATAA CGTCCGTGCCCTTCGCCGCCCTACTGGCCCCCCCCCCCGGCCCCCCCCCCCCCCCCCGAT CCAATACCCCGCCCGTTGGCACCCCTATCACCATACCCCCCCCCGCCGCTTCCCCCTACC CTTGCTGGAAATTAAAATACGGTANTACCCCCCCCCCCCATGTTCGCCCGCCTCCGCCCC CACCACTATATCTCCTGACCAACACCTATCCCCTCTCGCTTGCCGCGAAAGCACCTGCGT CGCCACACCACTCCACCCCCCCTCCCGCCTCTGCTCTCTG >OriGene 3' read for NM_030769 unedited
TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTTGAAAAATTT TATTTTTAATTCAAAAAGATTAAAATAAGGAGCAAAACTGATATGAATTTCAAATACTCT TTCTCTGCCAATGATCAAAACAATTAGAGATGAAATGAAAACAAAATCTTAGGTAATTAA CAGACTGGATAGTCAAGTTTAAAGTCAGTAAGGGTTAACTGTCAATATACTTTGATATCA GAAAGCTTATATCAAGGTTTGTTAGAAGGGAGATTAAACAGTTGTTGATACATTACACAG AAAGAAAAGAGTAAAAGGAGGTATGGAGAGAAAGTTCTGAGTCCTTTGGGTCCTCCCCAA ATCTTCTATCACTAAACAAAAGTCACTAGTTGCAAGTGTGACAGGATAATAACATTAGCC CCTGGGCTCACTGCAGAAAGGAAGTCAGCCTTCGATTTCAATAATTCAGGGTCCTGCGGG AGAGACAGTCTTCTCAGTGGAGGAAGGGGAAGTTTGTGGTGCCCAGAGGAAGAGTCCTTT GGTTCTTTGAAACTTTGAGTTTTGAGTTTTCTGTATAGAAAAAAGTGGAGACTTAAATTC ATCTTTGGGGAAGACTTTTTCCTTTGTTATGAGAAAGCCGCCCTTTACTTAGTTATAGGA ACTGAAACAAGTCCTCACCTGACCCAGTGTTTTTAAAGAGCGAAAAGTGGTGACATCGAA ATGAATTCAAAAGCATCCATTCTAGAGTTAGACTGAGCAGCCAAATGAGCTGGTGTATTT TGAGTTGTATACAGTTGAGACATGTTACAGTTTGGTATTAAAGTGCCTGCCTAAATGCAG TAGCTCAGAAGTTTCCTAGATTCCAATGAATATTTATTNAAATGAGACATATATAGTGGG TTAAAATTANACACTTTNAAATAGGANAGGGACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_030769 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030769.1, NP_110396.1 |
| RefSeq Size | 1552 bp |
| RefSeq ORF | 963 bp |
| Locus ID | 80896 |
| UniProt ID | Q9BXD5 |
| Domains | DHDPS |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism |
| Gene Summary | This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214546 | NPL (Myc-DDK-tagged)-Human N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 1 |
CN¥ 2,400.00 |
|
| RC214546L1 | Lenti ORF clone of Human N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 1, Myc-DDK-tagged |
CN¥ 4,800.00 |
|
| RC214546L2 | Lenti ORF clone of Human N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 1, mGFP tagged |
CN¥ 5,890.00 |
|
| RC214546L3 | Lenti ORF clone of Human N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 1, Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC214546L4 | Lenti ORF clone of Human N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 1, mGFP tagged |
CN¥ 5,890.00 |
|
| RG214546 | NPL (tGFP-tagged) - Human N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 1 |
CN¥ 4,000.00 |
