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DNMT3A (NM_153759) Human Untagged Clone
CAT＃: SC109167
DNMT3A (untagged)-Human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 2
CNY 5,304.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DNMT3A2; HESJAS; M.HsaIIIA; TBRS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_153759, the custom clone sequence may differ by one or more nucleotides
ATGGGGATCCTGGAGCGGGTTGTGAGAAGGAATGGGCGCGTGGATCGTAGCCTGAAAGACGAGTGTGATA CGGCTGAGAAGAAAGCCAAGGTCATTGCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTC TCAGAAGGTGGAGGAGGCCAGCCCTCCTGCTGTGCAGCAGCCCACTGACCCCGCATCCCCCACTGTGGCT ACCACGCCTGAGCCCGTGGGGTCCGATGCTGGGGACAAGAATGCCACCAAAGCAGGCGATGACGAGCCAG AGTACGAGGACGGCCGGGGCTTTGGCATTGGGGAGCTGGTGTGGGGGAAACTGCGGGGCTTCTCCTGGTG GCCAGGCCGCATTGTGTCTTGGTGGATGACGGGCCGGAGCCGAGCAGCTGAAGGCACCCGCTGGGTCATG TGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAGCTGATGCCGCTGAGCTCGTTTTGCAGTG CGTTCCACCAGGCCACGTACAACAAGCAGCCCATGTACCGCAAAGCCATCTACGAGGTCCTGCAGGTGGC CAGCAGCCGCGCGGGGAAGCTGTTCCCGGTGTGCCACGACAGCGATGAGAGTGACACTGCCAAGGCCGTG GAGGTGCAGAACAAGCCCATGATTGAATGGGCCCTGGGGGGCTTCCAGCCTTCTGGCCCTAAGGGCCTGG AGCCACCAGAAGAAGAGAAGAATCCCTACAAAGAAGTGTACACGGACATGTGGGTGGAACCTGAGGCAGC TGCCTACGCACCACCTCCACCAGCCAAAAAGCCCCGGAAGAGCACAGCGGAGAAGCCCAAGGTCAAGGAG ATTATTGATGAGCGCACAAGAGAGCGGCTGGTGTACGAGGTGCGGCAGAAGTGCCGGAACATTGAGGACA TCTGCATCTCCTGTGGGAGCCTCAATGTTACCCTGGAACACCCCCTCTTCGTTGGAGGAATGTGCCAAAA CTGCAAGAACTGCTTTCTGGAGTGTGCGTACCAGTACGACGACGACGGCTACCAGTCCTACTGCACCATC TGCTGTGGGGGCCGTGAGGTGCTCATGTGCGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTG TGGACCTCTTGGTGGGGCCGGGGGCTGCCCAGGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTG CGGGCACAAGGGTACCTACGGGCTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCAGATGTTCTTC GCTAATAACCACGACCAGGAATTTGACCCTCCAAAGGTTTACCCACCTGTCCCAGCTGAGAAGAGGAAGC CCATCCGGGTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGACTTGGGCATTCA GGTGGACCGCTACATTGCCTCGGAGGTGTGTGAGGACTCCATCACGGTGGGCATGGTGCGGCACCAGGGG AAGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGGAGTGGGGCCCATTCGATC TGGTGATTGGGGGCAGTCCCTGCAATGACCTCTCCATCGTCAACCCTGCTCGCAAGGGCCTCTACGAGGG CACTGGCCGGCTCTTCTTTGAGTTCTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGC CCCTTCTTCTGGCTCTTTGAGAATGTGGTGGCCATGGGCGTTAGTGACAAGAGGGACATCTCGCGATTTC TCGAGTCCAACCCTGTGATGATTGATGCCAAAGAAGTGTCAGCTGCACACAGGGCCCGCTACTTCTGGGG TAACCTTCCCGGTATGAACAGGCCGTTGGCATCCACTGTGAATGATAAGCTGGAGCTGCAGGAGTGTCTG GAGCATGGCAGGATAGCCAAGTTCAGCAAAGTGAGGACCATTACTACGAGGTCAAACTCCATAAAGCAGG GCAAAGACCAGCATTTTCCTGTCTTCATGAATGAGAAAGAGGACATCTTATGGTGCACTGAAATGGAAAG GGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTG GGCCGGTCATGGAGCGTGCCAGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAGTATTTTGCGTGTGTGT AA >OriGene 5' read for NM_153759 unedited
NNGGGGGGNCCNNNNNCCCCCCNNCCNNGGTTCAGATTTGTATACGACTCATATAGGCGG CCGCGNAATTCGCACCAGCCAAAAGGGCAGCTACTTCCAAAGCTTCAGGGCCGCGGCTCA CACCTGAGCGCGACTGCAGAGGGGCTGCACCTGGCCTTATGGGCTGAGAAGAAAGCCAAG GTCATTGCAGGAATGAATGCTGTGGAAGAAAACCAGGGGCCCGGGGAGTCTCAGAAGGTG GAGGAGGCCAGCCCTCCTGCTGTGCAGCAGCCCACTGACCCCGCATCCCCCACTGTGGCT ACCACGCCTGAGCCCGTGGGGTCCGATGCTGGGGACAAGAATGCCACCAAAGCAGGCGAT GACGAGCCAGAGTACGAGGACGGCCGGGGCTTTGGCATTGGGGAGCTGGTGTGGGGGAAA CTGCGGGGCTTCTCCTGGTGGCCAGGCCGCATTGTGTCTTGGTGGATGACGGGCCGGAGC CGAGCAGCTGAAGGCACCCGCTGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTG TGTGTTGAGAAGCTGATGCCGCTGAGCTCGTTTTGCAGTGCGTTCCACCAGGCCACGTAC AACAAGCAGCCCATGTACCGCANAGCCATCTACGAGGTCCTGCANGTGGCCAGCAGCCGC GCGGNGAAGCTGTTCCCGGTGTGCCACGACAGCGATGAGAGTGACACTGCCAAGGCCGTG GAGGTGCAGAACAAGCCCCCATGATGATGGGCCCTGNGGGGGCTTCAGCCTTCTGGCCCT AAGGCCTGNAGCCACCAGAGAAGAGAAAGATNCCCTACAAGAAGTGTACACGGACTGTTG GTGGAACCCTGAGCAGNTGCTACGCACCACTNCACAGNCAAAAGCCCCGNAGAGCCAGCG NAGAGCCCAGGTCAGGGAGATATGATGACGCACANNAAACGCTGTGTACCAGTGCGGCAN AAATGCCGAACATGAGAACTTGCTCTNCTGTGAAGCCTCAT >OriGene 3' read for NM_153759 unedited
TTTGCGCGTAGTCNTGGACCGCGCCGCATTTNANNGATCAGTTTTTTTTTTTTTTTTTTA CGTTTTGTATGTTTTTTTATTTGCTCCAGGTGGGGTTTTGACTGTCACTTTCCCACACTC TGGATTAGTTCTGATCCCACCACAAGGAGCCCTCGAATTGGCTAAAGTGAGAAACTGGGC CTGAAGACTCCGTACCCTCTGCCATCTTGCCGAGGGAGTCTCCTTTTAGAAAACAATCAA AGGGTTATTGCATGAGTCTGGATGAATCCCACTCTCAGCTGTCCACGGGCCCGACCACCT CATCTAGCCCCCTTTTTGGCAGGGAGAACCTGGCTCCCAAGTTCTCCTCCTTCACTTCGT TACAAACCAAGGGGAAGAGCCCACCGTGAGAACGCGCCATCTGCAAGCTGTCTCCCTTTC TCCATCCTTGGTGAAACCCTTTGCGCAGAAGGCAACGTGGAAGCTGATGTTTTTACTCAT CTCGCTGTTTGAAAGCACCATTATGAAGTCGGGTTGTACAGTAGTTAACAGTACCTTTTA TATATATATCTCATATCTATCATATATATATAAACTGTAAACAAGAGGTAACAGCGGCTT CTAGAAACTGATTTTCTTCAAGGTTTCCTGTGTGGTAGGCACCTGAAATACTGTAGAAAA ATGTCTTGTGTGGTGTTCTCGTCTCCCTGCTGCTAACTGGGTTCTGTTTTGCGTGACCAG GAATGGTGCTGGCACGACAGCTCANTGGCTGGGAAGGAATGCTGCCGCCTGAGTGTCTCT CTCTTTCCGTCCCCTGCTTGTGCTCCCTTCTGATCAGGCTAGAGACCACAAAAAAAAATT TAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_153759 |
| Insert Size | 3850 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153759.2, NP_715640.2 |
| RefSeq Size | 3608 bp |
| RefSeq ORF | 2172 bp |
| Locus ID | 1788 |
| UniProt ID | Q9Y6K1 |
| Domains | PWWP, DNA_methylase |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| Gene Summary | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (2) uses a downstream promoter compared to variants 1, 3, 4, and 7. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Methylation of LOXL1 promoter by DNMT3A in aged human skin fibroblasts
,Moulin, L;Cenizo, V;Nyamay Antu, A;Andre-Frei, V;Pain, S;Sommer, P;Debret, R;,
Rejuvenation Res
,PubMed ID 27396912
[DNMT3A]
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Other Versions
| SKU | Description | Size | Price |
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| RC220906 | DNMT3A (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 2 |
CNY 7,600.00 |
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| RC220906L3 | Lenti-ORF clone of DNMT3A (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 2 |
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| RC220906L4 | Lenti-ORF clone of DNMT3A (mGFP-tagged)-Human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 2 |
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| RG220906 | DNMT3A (tGFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 2 |
CNY 10,072.00 |
