DNMT3A Mouse Monoclonal Antibody [Clone ID: 64B1446]

Specifications

Product Data
Clone Name	64B1446
Applications	ChIP, CyTOF-ready, FC, ICC/IF, IHC, WB
Recommend Dilution	Western Blot, Flow Cytometry: 1 ug per million cells, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin: 5 ug/ml, Knockdown Validated: Validated for Knockdown from CiteAb, CyTOF-ready
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	DNA (cytosine-5-)-methyltransferase 3 alpha
Database Link	NP_783328 Entrez Gene 13435 MouseEntrez Gene 1788 Human UniProt ID Q9Y6K1
Background	Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Synonyms	DNMT3A2; M.HsaIIIA; TBRS
Note	Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24623306)
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cysteine and methionine metabolism, Metabolic pathways

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