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BDNF (NM_170733) Human Untagged Clone
CAT＃: SC108977
BDNF (untagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 5
CNY 3,600.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANON2; BULN2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_170733 edited
GAATTCGGCACGAGGATTACCTCCGCCATGCAATTTCCACTATCAATAATTTAACTTCTT TGCTGCAGAACAGAAGGAGTACATACCGGGCACCAAAGACTCGCGCCCCCTCCCCCCTTT AATTAAGCGAAGGGAACGTGAAAAAATAATAGAGTGTGGGAGTTTTGGGGCCGAAGTCTT TCCCGGAGCAGCTGCCTTGATGGTTACTTTGACAAGTAGTGACTGAAAAGTTCCACCAGG TGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTTGGTTGCATGA AGGCTGCCCCCATGAAAGAAGCAAACATCCGAGGACAAGGTGGCTTGGCCTACCCAGGTG TGCGGACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAAGGCAGGTTCAAGAGGCTTGA CATCATTGGCTGACACTTTCGAACACGTGATAGAAGAGCTGTTGGATGAGGACCAGAAAG TTCGGCCCAATGAAGAAAACAATAAGGACGCAGACTTGTACACGTCCAGGGTGATGCTCA GTAGTCAAGTGCCTTTGGAGCCTCCTCTTCTCTTTCTGCTGGAGGAATACAAAAATTACC TAGATGCTGCAAACATGTCCATGAGGGTCCGGCGCCACTCTGACCCTGCCCGCCGAGGGG AGCTGAGCGTGTGTGACAGTATTAGTGAGTGGGTAACGGCGGCAGACAAAAAGACTGCAG TGGACATGTCGGGCGGGACGGTCACAGTCCTTGAAAAGGTCCCTGTATCAAAAGGCCAAC TGAAGCAATACTTCTACGAGACCAAGTGCAATCCCATGGGTTACACAAAAGAAGGCTGCA GGGGCATAGACAAAAGGCATTGGAACTCCCAGTGCCGAACTACCCAGTCGTACGTGCGGG CCCTTACCATGGATAGCAAAAAGAGAATTGGCTGGCGATTCATAAGGATAGACACTTCTT GTGTATGTACATTGACCATTAAAAGGGGAAGATAGTGGATTTATGTTGTATAGATTAGAT TATATTGAGACAAAAATTATCTATTTGTATATATACATAACAGGGTAAATTATTCAGTTA AGAAAAAAATAATTTTATGAACTGCATGTATAAATGAAGTTTATACAGTACAGTGGTTCT ACAATCTATTTATTGGACATGTCCATGACCAGAAGGGAAACAGTCATTTGCGCACAACTT AAAAAGTCTGCATTACATTCCTTGATAATGTTGTGGTTTGTTGCCGTTGCCAAGAACTGA AAACATAAAAAGTTAAAAAAAATAATAAATTGCATGCTGCTTTAATTGTGAATTGAAAAA AAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_170733 unedited
AAAACCCATTCCGCACGAGGATTACCTCCTCCATGCAATTTCCACTATCAATAATTTAAC TTCTTTGCTGCAGAACAGAAGGAGTACATACCGGGCACCAAAGACTCGCGCCCCCTCCCC CCTTTAATTAAGCGAAGGGAACGTGAAAAAATAATAGAGTGTGGGAGTTTTGGGGCCTAA GTCNNTCCCGGAGCAGCTGCCTTGATGGTTACTTTGACAAGTAGTGACTGAAAAGTTCCA CCAGGTGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTTGGTTG CATGAAGGCTGCCCCCATGAAAGAAGCAAACATCCGAGGACAAGGTGGCTTGGCCTACCC AGGTGTGCGGACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAAGGCAGGTTCAAGAGG CTTGACATCATTGGCTGACACTTTCGAACACGTGATAGAAGAGCTGTTGGATGAGGACCA GAAAGTTCGGCCCAATGAAGAAAACAATAAGGACGCAGACTTGTACACGTCCAGGGTGAT GCTCAGTAGTCAAGTGCCTTTGGAGCCTCCTCTTCTCTTTCTGCTGGAGGAATACAAAAA TTACCTAGATGCTGCAAACATGTCCATGAGGGTCCGGCGCCACTCTGACCCTGCCCGCCG AGGGGAGCTGAGCGTGTGTGACAGTATTAGTGAGTGGGTAACGGCGGCAGACANAAGACT GCAGTGGACATGTCGGGCGGGACGGTCACAGTCCTTTGAAAGGTCCCTGTATCAAAAGGC CAACTGAAGCAATACTTCTACGAGACCAAGTGCCATCCCATGGGTTACCAAAAGAAGGCT GCGGGGGCATAGACAAAAAGCATTGGAACTCCCAGTGGCC >OriGene 3' read for NM_170733 unedited
CCCCCCCCATGNTTGNACCCGGCCGCATTCTAGGATCGGTTTTTTTTTTTTTTTTTTCAA TTCACAATTATATGCGCATGCAATTTATTATTTTTTTTAACTTTTTATGTTTTCAGTTCT TGGCAACGGCAACAAACCACAACATTATCAAGGAATGTAATGCAGACTTTTTAAGTTGTG CGCAAATGACTGTTTCCCTTCTGGTCATGGACATGTCCAATAAATAGATTGTAGAACCAC TGTACTGTATAAACTTCATTTATACATGCAGTTCATAAAATTATTTTTTTCTTAACTGAA TAATTTACCCTGTTATGTATATATACAAATAGATAATTTTTGTCTCAATATAATCTAATC TATACAACATAAATCCACTATCTTCCCCTTTTAATGGTCAATGTACATACACAAGAAGTG TCTATCCTTATGAATCGCCAGCCAATTCTCTTTTTGCTATCCATGGTAAGGGCCCGCACG TACGACTGGGTAGTTCGGCACTGGGAGTTCCAATGCCTTTTGTCTATGCCCCTGCAGCCT TCTTTTGTGTAACCCATGGGATTGCACTTGGTCTCGTAAAAGTATTGCTTCAGTTGGCCT TTTGATACAGGGACCTTTTCAAGGACTGTGACCGTCCCGCCCGACATGTCCACTGCAGTC TTTTTGTCTGCCGCCGTTACCCACTCACTAATACTGTCACACACGCTCAGCTCCCCTCGG CGGGCAGGGTCAGAGTGGCGCCGGACCCTCATGGACATGTTTGCAGCATCTAGGTAACTT TTGTATTCCTCCAGCAGAAAGAGAGCAGGAGGCTCCAAAGCACTTGACTACTGAGCATCA CCCTGGACGTGTACAAGNTTGCGTCCTTAATGTTTCCTCATTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_170733 |
| Insert Size | 1330 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_170733.2, NP_733929.1 |
| RefSeq Size | 4016 bp |
| RefSeq ORF | 744 bp |
| Locus ID | 627 |
| UniProt ID | P23560 |
| Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane |
| Protein Pathways | Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway |
| Gene Summary | This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (5), also known as IV, BDNF4, or 3-5, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Locally Activating TrkB Receptor Generates Actin Waves and Specifies Axonal Fate.
,null,
Cell chemical biology
,PubMed ID 31678045
[BDNF]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212722 | BDNF (Myc-DDK-tagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 5 |
CNY 3,600.00 |
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| RC212722L1 | Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 5, Myc-DDK-tagged |
CNY 6,000.00 |
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| RC212722L2 | Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 5, mGFP tagged |
CNY 5,890.00 |
|
| RC212722L3 | Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 5, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212722L4 | Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 5, mGFP tagged |
CNY 5,890.00 |
|
| RG212722 | BDNF (tGFP-tagged) - Human brain-derived neurotrophic factor (BDNF), transcript variant 5 |
CNY 5,200.00 |
