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BCKDHB (NM_000056) Human Untagged Clone
CAT＃: SC108972
BCKDHB (untagged)-Human branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), nuclear gene encoding mitochondrial protein, transcript variant 2
CN¥ 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCKDE1B; BCKDH E1-beta; E1B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_000056, the custom clone sequence may differ by one or more nucleotides
ATGGCGGTTGTAGCGGCGGCTGCCGGCTGGCTACTCAGGCTCAGGGCGGCAGGGGCTGAGGGGCACTGGC GTCGGCTTCCTGGCGCGGGGCTGGCGCGGGGCTTTTTGCACCCCGCCGCGACTGTCGAGGATGCGGCCCA GAGGCGGCAGGTGGCTCATTTTACTTTCCAGCCAGATCCGGAGCCCCGGGAGTACGGGCAAACTCAGAAA ATGAATCTTTTCCAGTCTGTAACAAGTGCCTTGGATAACTCATTGGCCAAAGATCCTACTGCAGTAATAT TTGGTGAAGATGTTGCCTTTGGTGGAGTCTTTAGATGCACTGTTGGCTTGCGAGACAAATATGGAAAAGA TAGAGTTTTTAATACCCCATTGTGTGAACAAGGAATTGTTGGATTTGGAATCGGAATTGCGGTCACTGGA GCTACTGCCATTGCGGAAATTCAGTTTGCAGATTATATTTTCCCTGCATTTGATCAGATTGTTAATGAAG CTGCCAAGTATCGCTATCGCTCTGGGGATCTTTTTAACTGTGGAAGCCTCACTATCCGGTCCCCTTGGGG CTGTGTTGGTCATGGGGCTCTCTATCATTCTCAGAGTCCTGAAGCATTTTTTGCCCATTGCCCAGGAATC AAGGTGGTTATACCCAGAAGCCCTTTCCAGGCCAAAGGACTTCTTTTGTCATGCATAGAGGATAAAAATC CTTGTATATTTTTTGAACCTAAAATACTTTACAGGGCAGCAGCGGAAGAAGTCCCTATAGAACCATACAA CATCCCACTGTCCCAGGCCGAAGTCATACAGGAAGGGAGTGATGTTACTCTAGTTGCCTGGGGCACTCAG GTTCATGTGATCCGAGAGGTAGCTTCCATGGCAAAAGAAAAGCTTGGAGTGTCTTGTGAAGTCATTGATC TGAGGACTATAATACCTTGGGATGTGGACACAATTTGTAAGTCTGTGATCAAAACAGGGCGACTGCTAAT CAGTCACGAGGCTCCCTTGACAGGCGGCTTTGCATCGGAAATCAGCTCTACAGTTCAGGAGGAATGTTTC TTGAACCTAGAGGCTCCTATATCAAGAGTATGTGGTTATGACACACCATTTCCTCACATTTTTGAACCAT TCTACATCCCAGACAAATGGAAGTGTTATGATGCCCTTCGAAAAATGATCAACTATTGA >OriGene 5' read for NM_000056 unedited
ATTTTGTAAACCGACTTTCTATAGGCGGCCGCGCATTCGGCACGAGGGCGGGGATGGCGG TTGTAGCGGCGGCTGCCGGCTGGCTACTCAGGCTCAGGGCGGCAGGGGCTGAGGGGCACT GGCGTCGGCTTCCTGGCGCGGGGCTGGCGCGGGGCTTTTTGCACCCCGCCGCGACTGTCG AGGATGCGGCCCAGAGGCGGCAGGGCAAACTCAGAAAATGAATCTTTTCCAGTCTGTAAC AAGTGCCTTGGATAACTCATTGGCCAAAGATCCTACTGCAGTAATATTTGGTGAAGATGT TGCCTTTGGTGGAGTCTTTAGATGCACTGTTGGCTTGCGAGACAAATATGGAAAAGATAG AGTTTTTAATACCCCATTGTGTGAACAAGGAATTGTTGGATTTGGAATCGGAATTGCGGT CACTGGAGCTACTGCCATTGCGGAAATTCAGTTTGCAGATTATATTTTCCCTGCATTTGA TCAGATTGTTAATGAAGCTGCCAAGTATCGCTATCGCTCTGGGGATCTTTTTAACTGTGG AAGCCTCACTATCCGGTCCCCTTGGGGCTGTGTTGGTCATGGGGCTCTCTATCATTCTCA GAGTCCTGAAGCATTTTTTGCCCATTGCCCAGGAATCAAGGTGGTTATACCCAGAAGCCC TTTCNCAGCCAAAGGACTTCTTTTGTCATGCATAGAGGATAAAAATCCTTGTATATTTTT TGGACCTAANATACTTTACAGGGCAGCAGCGGAAAGAGTNCCTATAGAACCATACAACAT CCCACTGTCCCAGNCCGAAGTCATACAGGAAGGGAGTGATGNNTACTCTAGTGCCTGGGG GCACTCAGNTCATGTGATCCGAGAGGTAGCTTCCATGGCANAAGAAAGCTNGGAGTGTC >OriGene 3' read for NM_000056 unedited
CGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTGACAATTTGATTTTACTTTATT GAATACTATCACACACATAATATTTACAGATGTAATTAAACAGAATTTATAAGATTACCA TGTGGAATATTTTTCAAAGTTAATCTGAAACTCTTAATGAAAATCATCAATACATGTGTG TTACAATACAATAATAGAATGTAGAACTTTTCAGCCAATATCATGATGGTTCATTTAATA AAAACTCTCTTTTCAAAAAGAGGTAAGTCGGAGGAAATATAAAAAAAAATTCAGAAAAAA TATTTCCATATCTAGTCATAATCTTCCAGCTTTTCTGTAAAATAAATTATAGACTTTCTC AATTGCATACATGACTATTTTCTTAAATACCATATTTAACTTTTAGCATATTGGAGTTGT TTTTCTTTTATCGTATTTTCAGCATAGGTTTAAATGTTTTTCTTATGTTTCAAAAGGAAT ACATAATCTAAATTTAAAACCACATCTGAATATTACTTTAAAAATAGCAACGAGGCACGT GGCCACCATCACTACCTTGGCTCTTTATTACGGCTGGTCCACGGAAGGGAACCACATGGA CAACAAAAGTGAGGGAGTCCTCTAACCAGTTCTAAGTGTGATTTATTGTAGATGCCAGTT GCTTTCACAGACATTAAGAAGACATCAGANATATGGCATCTACACCGGTATGTCTGAGAG ATACACGCATCTGTGGGCTAATGGTCCTAACTTCCATCTATAACANTATTCTTAGACTAG AACAGACAGAAATGTNAGGATTATGTAGCACAGTGACTATGAGGGAGAGACTACTCTCTA TCCGACAATGTAAAGGCGTTCACTCTGATTTCAGGAGCTAGATTACTACCATACACAAGT CAGAATCTAGCTTTCTGACTTTAAAGNNAAGATGTGCTTTTTCAGAGTGATTTGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000056 |
| Insert Size | 3340 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000056.2, NP_000047.1 |
| RefSeq Size | 1552 bp |
| RefSeq ORF | 1179 bp |
| Locus ID | 594 |
| UniProt ID | P21953 |
| Domains | transket_pyr, transketolase_C |
| Protein Pathways | Metabolic pathways, Valine, leucine and isoleucine degradation |
| Gene Summary | This gene encodes the E1 beta subunit of branched-chain keto acid dehydrogenase, which is a multienzyme complex associated with the inner membrane of mitochondria. This enzyme complex functions in the catabolism of branched-chain amino acids. Mutations in this gene have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation and feeding problems. Alternative splicing at this locus results in multiple transcript variants. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (2) lacks an alternate segment in the 3' UTR compared to transcript variant 1. Both variants 1 and 2 encode the same protein (isoform 1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218846 | BCKDHB (Myc-DDK-tagged)-Human branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CN¥ 3,656.00 |
|
| RC218846L3 | Lenti ORF clone of Human branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC218846L4 | Lenti ORF clone of Human branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CN¥ 5,890.00 |
|
| RG218846 | BCKDHB (tGFP-tagged) - Human branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CN¥ 5,256.00 |
