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Adducin 2 (ADD2) (NM_001617) Human Untagged Clone
CAT＃: SC108902
ADD2 (untagged)-Human adducin 2 (beta) (ADD2), transcript variant 1
CNY 5,320.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADDB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108902 sequence for NM_001617 edited (data generated by NextGen Sequencing)
ATGAGCGAAGAGACGGTCCCCGAGGCTGCCTCGCCGCCGCCCCCGCAGGGGCAGCCTTAC TTTGACCGCTTCTCAGAGGACGACCCCGAGTACATGCGCCTTCGCAACCGGGCGGCGGAC CTGCGGCAGGACTTCAACCTGATGGAGCAGAAGAAGCGCGTCACCATGATCCTGCAGAGT CCCTCTTTCAGGGAGGAGCTGGAAGGCCTCATCCAGGAGCAGATGAAGAAGGGGAACAAC TCCTCCAACATCTGGGCCCTGCGACAGATCGCGGACTTCATGGCCAGCACCTCCCACGCA GTCTTCCCGACATCTTCCATGAATGTCTCCATGATGACGCCTATCAATGACCTCCACACA GCTGACTCCCTGAACCTGGCCAAAGGGGAGCGGCTCATGCGGTGCAAGATCAGCAGTGTC TACCGACTCCTGGACCTCTATGGCTGGGCCCAGCTGAGTGACACCTATGTCACGTTGAGA GTCAGCAAGGAGCAGGACCACTTCCTGATCAGCCCTAAGGGAGTTTCTTGCAGTGAAGTC ACAGCGTCCAGCCTGATCAAGGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGC TGCTTCCCAGTGGACACCACAGGCTTCTGTCTGCACTCGGCCATCTATGCAGCGAGGCCC GACGTGCGCTGCATCATCCACCTGCACACACCGGCCACAGCAGCGGTGTCGGCCATGAAG TGGGGCCTCCTGCCTGTCTCCCACAATGCCCTGCTGGTGGGGGACATGGCCTATTATGAC TTCAATGGGGAAATGGAGCAGGAAGCCGATCGGATCAACCTGCAGAAGTGCCTTGGACCC ACCTGCAAGATCCTGGTGCTAAGAAACCATGGAGTGGTTGCTCTGGGTGACACGGTAGAG GAGGCATTTTACAAGATCTTCCACCTGCAGGCTGCATGTGAGATACAGGTGTCGGCTCTG TCCAGTGCCGGGGGAGTGGAGAACCTCATCCTCCTGGAGCAGGAGAAGCACCGGCCCCAT GAGGTGGGCTCCGTGCAGTGGGCCGGGAGCACCTTTGGGCCTATGCAGAAGAGTCGGCTG GGGGAGCATGAGTTTGAGGCCCTCATGAGGATGCTGGACAACCTGGGCTACAGAACAGGT TACACGTATCGCCACCCCTTTGTTCAAGAGAAAACCAAACACAAAAGTGAGGTGGAGATT CCAGCCACGGTCACAGCCTTCGTGTTTGAGGAGGACGGTGCCCCGGTGCCCGCCCTGCGA CAGCATGCCCAGAAGCAGCAGAAGGAGAAGACCCGCTGGCTCAATACGCCCAACACCTAC CTGCGGGTCAATGTGGCCGATGAGGTCCAGAGGAGCATGGGCAGCCCCCGACCCAAGACC ACGTGGATGAAGGCTGACGAGGTGGAGAAATCCAGCAGTGGCATGCCGATTCGCATCGAA AACCCAAACCAATTTGTGCCTCTCTATACTGACCCCCAGGAAGTACTGGAGATGAGGAAC AAGATTCGAGAACAAAACCGACAAGATGTGAAGTCAGCGGGGCCTCAGTCCCAGCTCCTG GCGAGCGTCATTGCCGAGAAGAGCCGAAGCCCGTCTACAGAGAGCCAGCTGATGTCCAAG GGAGACGAGGATACCAAAGACGATTCAGAGGAGACGGTGCCCAACCCCTTCAGCCAACTC ACTGACCAGGAGTTGGAGGAGTACAAGAAAGAGGTGGAGAGGAAGAAACTAGAACTTGAT GGAGAGAAAGAAACTGCCCCAGAAGAGCCTGGCTCACCTGCAAAGTCTGCACCTGCTTCT CCAGTGCAGAGCCCAGCGAAGGAGGCAGAGACAAAGAGCCCTTTAGTCTCTCCTTCCAAG TCTTTAGAGGAAGGTACTAAGAAGACAGAAACAAGCAAAGCCGCCACCACAGAGCCCGAA ACAACCCAGCCGGAAGGGGTGGTGGTCAACGGGAGGGAGGAGGAGCAGACGGCAGAGGAA ATCCTCAGCAAAGGCCTGAGCCAGATGACCACCAGTGCTGACACGGATGTTGATACCTCT AAGGACAAAACCGAGTCGGTCACCAGCGGCCCCATGTCCCCAGAGGGCTCACCTTCCAAG TCTCCCTCAAAGAAGAAAAAGAAATTCCGAACCCCCTCCTTCCTGAAAAAGAGCAAAAAG AAGGAGAAAGTGGAGTCCTGA Clone variation with respect to NM_001617.3 >OriGene 5' read for NM_001617 unedited
GGTCANAAATTGTAAACGACTCATATAGGGCGGCCGCGAATCGGCACGAGGATGGCTTTA ATTCTGACGGCAGGGCTGTGAGGGACTAGCGGGAACCCGAGCCTTTTGTCAAGGAACTGC GGCGTCGGTGGCCAGTCAACCCCGCCGCCGCGGAGCCGCTGCACTGCTGGGGGATCTCCC AGCAGCTCTGACGAGCGCGGGCTGCAGCATGGGCAGAAAACGCTGCTCTGCAGATTAGCT GGGTGGATTTTTTAAGCGCACCCCACCCCCCAAACCCATAAAATAACAAAACCAACCCGC AGTGGCCGACCGGAGATAGCTAAGATGCCGCGCAGGAGTTTCCACCTGGATGTTTGAGGT TGTGTAGATGTGGCCGGCACCCTTGAGAGTGGAGCTAGGGGGTGCAGACTGAGCAGTGAA CAGAAGGAGCCTTGGACAGGGCTGGGCCAGCCTCCCGAGTTCCAGGAGCGAATTGCAAAC CCACCGGGAAAATGAGCGAAGAGACGGTCCCCGAGGCTGCCTCGCCGCCGCCCCCGCAGG GGCAGCCTTACTTTGACCGCTTCTCAGAGGACGACCCCGAGTACATGCGCCTTCGCACCG GGCNGCGGACCTGCGGCAGGACTTTCACCTGATGGAGCAGAAGAAGCGCGTCACCATGAT CCTGCAGAGTCCCTCTTTCAGGGNAGAGCTGGGANGNCTCATCCAGGAGCAGATGAAGAA GGGGGAACACTNNCTCCACATCTGGGGCCCTGCGACAGATCGCGGACTTCATGGCCAGCA CCTCCAACGCAGTCTTCCNGGACATCTCCATGAATGTCTCCATGATGACGCCCTATCATG ACCTNCACACAGCTGACTCCCTGAACCTGGCAAAAGGGGGAGCGCTCATGCGTGCAGATC AGCAGTGTCTACCGACTCCTGGACCTCTATGGCTGGGCCCAGCTGAT >OriGene 3' read for NM_001617 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTNNTCTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTAAAAAAAAGGGTTGGTCAAATTTTAAGGAGCCACCAAAGGCTCTTTAT TAAAGGCCCAAGGGAAAAACTTGTAAAAATAGTGTTGGATTTAAAAACATTAACTGGGGC CTTTACCAGGCCCAAGGAGGGACAGATCGGGACATGGACGGGGTTCCCCAGATTTTTTTT GAAAGTTGTCGCAAGAGGATTAATCTTGGAGGGTTAATTTTTGATTCTCTTCTGCTGGAC AAAGACGGATTCGGTTGGACCCCACAAGGGGGCACGTCAGGGTGCCAAAAGTAATACCCC TCTTCCCCCTTCTACCTATTCGTTCCTGTTGCCCGGTTACGTTTCGTTCCCCCTCTTCCC CCCCCGCGCTTCGCCTATTTACCGCAACGTCTCTTCCCTTGTTGGAGCCGAACTTTCGTC TTTCCCCCGTACTACGAAAGTTCTCTCCTACCCCCCCTTCACTTGCCCCCCCTTTTCCCC AATTTCTCCTTAAAAGAACCCTCACACCCACCCCCCCCCGTCCTTACACACCCTGCTCCT CTCCTTTTTCCCGTCCCGCTTTTTCTTGGTTTCTGATTTTCCGTTCTCACCTTGCGTCGT CTTCCCGCACTTTATTTCCGCCCCTTCTCCCCACGCCGCTTTTCTTTCCCTCCCCCTTTC GCTTCTTTAATCCCCCTCTCTCGCCTTATTGTCTTCGTACATTATCTCCCACCGGTTGCC TTCTCAGTTCTCCCACGGCGGCCGTAACATCACGCGTCAAGTTACTATCTGCTCCTTTTT CCTCTTTACCCGCCCATCACACGATTCGCGCCCTTCCCACATTGTTCGACTCTCGCACTG TTCTCTTTTTTGTTTCTGTACCCTTCCCGCACGCACGCTCTCCCGCTCCACTATTCTTTA CACCTCCCCTCTTTCTCCGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001617 |
| Insert Size | 3780 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001617.1, NP_001608.1 |
| RefSeq Size | 3932 bp |
| RefSeq ORF | 2181 bp |
| Locus ID | 119 |
| UniProt ID | P35612 |
| Domains | Aldolase_II |
| Gene Summary | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) encodes the longest isoform (a). Both variants 1 and 5 encode the same isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209517 | ADD2 (Myc-DDK-tagged)-Human adducin 2 (beta) (ADD2), transcript variant 1 |
CNY 5,320.00 |
|
| RC209517L3 | Lenti ORF clone of Human adducin 2 (beta) (ADD2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209517L4 | Lenti ORF clone of Human adducin 2 (beta) (ADD2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG209517 | ADD2 (tGFP-tagged) - Human adducin 2 (beta) (ADD2), transcript variant 1 |
CNY 4,370.00 |
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