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Neurofilament (NEFM) (NM_005382) Human Untagged Clone
CAT＃: SC108545
NEFM (untagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 1
CNY 6,720.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NEF3; NF-M; NFM |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005382, the custom clone sequence may differ by one or more nucleotides
ATGAGCTACACGTTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTAACCGAGACCCGCTCGAGCT TCAGCCGCGTCAGCGGCTCCCCGTCCAGTGGCTTCCGCTCGCAGTCGTGGTCCCGCGGCTCGCCCAGCAC CGTGTCCTCCTCCTATAAGCGCAGCATGCTCGCCCCGCGCCTCGCTTACAGCTCGGCCATGCTCAGCTCC GCCGAGAGCAGCCTTGACTTCAGCCAGTCCTCGTCCCTGCTCAACGGCGGCTCCGGACCCGGCGGCGACT ACAAGCTGTCCCGCTCCAACGAGAAGGAGCAGCTGCAGGGGCTGAACGACCGCTTTGCCGGCTACATAGA GAAGGTGCACTACCTGGAGCAGCAGAATAAGGAGATTGAGGCGGAGATCCAGGCGCTGCGGCAGAAGCAG GCCTCGCACGCCCAGCTGGGCGACGCGTACGACCAGGAGATCCGCGAGCTGCGCGCCACCCTGGAGATGG TGAACCACGAGAAGGCTCAGGTGCAGCTGGACTCGGACCACCTGGAGGAAGACATCCACCGGCTCAAGGA GCGCTTTGAGGAGGAGGCGCGGTTGCGCGACGACACTGAGGCGGCCATCCGCGCGCTGCGCAAAGACATC GAGGAGGCGTCGCTGGTCAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCCTTCC TGCGGAGCAACCACGAGGAGGAGGTGGCCGACCTTCTGGCCCAGATCCAGGCATCGCACATCACGGTGGA GCGCAAAGACTACCTGAAGACAGACATCTCGACGGCGCTGAAGGAAATCCGCTCCCAGCTCGAAAGCCAC TCAGACCAGAATATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCG AGCAGAACAAGGAGGCCATCCGCTCCGCCAAGGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAA GAGCATCGAGCTAGAGTCGGTGCGCGGCACCAAGGAGTCCCTGGAGCGGCAGCTCAGCGACATCGAGGAG CGCCACAACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGCTGGAAAATGAGCTTCGGGGCACAA AGTGGGAAATGGCTCGTCATTTGCGCGAATACCAGGACCTCCTCAACGTCAAGATGGCTCTGGATATAGA AATCGCTGCGTACAGAAAACTCCTGGAGGGTGAAGAGACTAGATTTAGCACATTTGCAGGAAGCATCACT GGGCCACTGTATACACACCGACCCCCAATCACAATATCCAGTAAGATTCAGAAACCCAAGGTGGAAGCTC CCAAGCTTAAGGTCCAACACAAATTTGTCGAGGAGATCATAGAGGAAACCAAAGTGGAGGATGAGAAGTC AGAAATGGAAGAGGCCCTGACAGCCATTACAGAGGAATTGGCCGTTTCCATGAAGGAAGAGAAGAAAGAA GCAGCAGAAGAAAAGGAAGAGGAACCCGAAGCTGAAGAAGAAGAAGTAGCTGCCAAAAAGTCTCCAGTGA AAGCAACTGCACCTGAAGTTAAAGAAGAGGAAGGGGAAAAGGAGGAAGAAGAAGGCCAGGAAGAAGAGGA GGAAGAAGATGAGGGAGCTAAGTCAGACCAAGCCGAAGAGGGAGGATCCGAGAAGGAAGGCTCTAGTGAA AAAGAGGAAGGTGAGCAGGAAGAAGGAGAAACAGAAGCTGAAGCTGAAGGAGAGGAAGCCGAAGCTAAAG AGGAAAAGAAAGTGGAGGAAAAGAGTGAGGAAGTGGCTACCAAGGAGGAGCTGGTGGCAGATGCCAAGGT GGAAAAGCCAGAAAAAGCCAAGTCTCCTGTGCCAAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTG CCCAAGTCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGCCCAAGTCACCAGTGGAAGAGAAAGGCAAGT CTCCTGTGCCGAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGTCAAAATCACCAGTGGAAGAGAA AGCCAAATCTCCTGTGCCAAAATCACCAGTGGAAGAGGCAAAGTCAAAAGCAGAAGTGGGGAAAGGTGAA CAGAAAGAGGAAGAAGAAAAGGAAGTCAAGGAAGCTCCCAAGGAAGAGAAGGTAGAGAAAAAGGAAGAGA AACCAAAGGATGTGCCAGAGAAGAAGAAAGCTGAGTCCCCTGTAAAGGAGGAAGCTGTGGCAGAGGTGGT CACCATCACCAAATCGGTAAAGGTGCACTTGGAGAAAGAGACCAAAGAAGAGGGGAAGCCACTGCAGCAG GAGAAAGAGAAGGAGAAAGCGGGAGGAGAGGGAGGAAGTGAGGAGGAAGGGAGTGATAAAGGTGCCAAGG GATCCAGGAAGGAAGACATAGCTGTCAATGGGGAGGTAGAAGGAAAAGAGGAGGTAGAGCAGGAGACCAA GGAAAAAGGCAGTGGGAGGGAAGAGGAGAAAGGCGTTGTCACCAATGGCCTAGACTTGAGCCCAGCAGAT GAAAAGAAGGGGGGTGATAAAAGTGAGGAGAAAGTGGTGGTGACCAAAACGGTAGAAAAAATCACCAGTG AGGGGGGAGATGGTGCTACCAAATACATCACTAAATCTGTAACCGTCACTCAAAAGGTTGAAGAGCATGA AGAGACCTTTGAGGAGAAACTAGTGTCTACTAAAAAGGTAGAAAAAGTCACTTCACACGCCATAGTAAAG GAAGTCACCCAGAGTGACTAA >OriGene 5' read for NM_005382 unedited
TTGGATTTTGTATCCGACTTATATGGGCGGCCCGCGATTCGGCACGAGGCGCCCCAAGGC CTCCAAGTATGAGCTACACGTTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTAA CCGAGACCCGCTCGAGCTTCAGCCGCGTCAGCGGCTCCCCGTCCAGTGGCTTCCGCTCGC AGTCGTGGTCCCGCGGCTCGCCCAGCACCGTGTCCTCCTCCTATAAGCGCAGCATGCTCG CCCCGCGCCTCGCTTACAGCTCGGCCATGCTCAGCTCCGCCGAGAGCAGCCTTGACTTCA GCCAGTCCTCGTCCCTGCTCAACGGCGGCTCCGGACCCGGCGGCGACTACAAGCTGTCCC GCTCCAACGAGAAGGAGCAGCTGCAGGGGCTGAACGACCGCTTTGCCGGCTACATAGAGA AGGTGCACTACCTGGAGCAGCAGAATAAGGAGATTGAGGCGGAGATCCAGGCGCTGCGGC AGAAGCAGGCCTCGCACGCCCAGCTGGGCGACGCGTACGACCAGGAGATCCGCGAGCTGC GCGCCACCCTGGAGATGGTGAACCACGAGAAGGCTCAGGTGCAGCTGGACTCGGACCACC TGGAGGAAGACATCCACCGGCTCAAGGAGCGCTTTGGGNAGGGAGGCCNCCGGTTGCGCC GACGACACTGGATGCGGCCATTCCGCGCGCTGCGCATAGACATCGAGGAGGCCGTCGCTG GTCAAGGTGGNAGCTGGACAATGATGGTGCAGTCGCTGCAGNATGAAGGTGCCCTTCCTG CGGAGCATCCCGAGGGAGGAGGGTGCCCGGACTTCTGGCCCAGATCCAGGCATCGNACAT CACGGTGGGAGCGCAAAGACTAACCTGAGACAGACTTCTCGGCGGCGCCGAAGGAAATCC GCTCCCAGCTCGAAAGCCATTAAACCAAATTTGCCCAGGCCGATAATGGTTCAAATGCCG GTCCCCA >OriGene 3' read for NM_005382 unedited
CTTTGGCCCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTATGTTTCCA ATATGACCTTTATTGAGCAAGTACTGCTGTGACGTTTAACATCGATCAACATAAGATTGC AAACTGTGCATGTCAACGAGGTTCTCCCATGAACAAAAGTCCATTTTAAATGCACCAGTG AGGTAAACATAATTATTCATATATAGCTTTCTTAAGGCTGAGTTCTGTGTTTCATTTATT GTTTTTGGCTCAGTTGGTACTTTGCATAATACATCTCAAGACATCCCCCCTTAAGTGACC CCCTTCCAACAGCTTAGGAAATCGGCAAATTTCCCAGGTACATACAATATCATGCATTTG AATCCCCCAAAGACTGAGGAGGGAGCACCCTGAGCTTGCATGCAGTCCCCTCATATTGCA CAAAGTAAAATACAATGTCTGGAGCCCCCATGGGAGAACCCGTTCTGTTTTGAAGCTGCC AATCACATGCATTTTGAAATTGTCATATGGCTTAACCTTTTGCAATGGACTCAAATCTTA GTCACTCTGGGTGACTTCCTTTACTATGGCGTGTGAAGTGACTTTTTCTACCTTTTTAGT AGACACTAGTTTCTCCTCAAAGGTCTCTTCATGCTCTTCAACCTTTTGAGTGACGGTTAC AGATTTAGTGATGTATTTGGTAGCACCATCTCCCCCCTCACTGGTGATTTTTTCTACCGT TTTGGTCACCACCACTTTCTCCTCACTTTTATCACCCCCCTTCTTTTCATCTGCTGGGCT CAAGTCTAGGCCATTGGTGACAACGCCTTTTCTCCTCTTTCCTCCCACTGCCTTTTTTCC TTGGGNCTCCTGCTNCTACCTNNCTCTTTTCCTTCTACCTTCCCATTGACGCTATGNCTT CCTTCCTGGATCCTTTGGACCTTTAATACTACCTTCCTCCTAATTACCTCCTTTCTTCCC GCTTTTTCCTTTTCTTTTTCCTGGTGGAGGGGCTCCCCCTTTCTCTTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005382 |
| Insert Size | 3330 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005382.1, NP_005373.1 |
| RefSeq Size | 3238 bp |
| RefSeq ORF | 2751 bp |
| Locus ID | 4741 |
| UniProt ID | P07197 |
| Domains | filament, filament_head |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS) |
| Gene Summary | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224475 | NEFM (Myc-DDK-tagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 1 |
CNY 10,064.00 |
|
| RC224475L1 | Lenti-ORF clone of NEFM (Myc-DDK-tagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 1 |
CNY 12,464.00 |
|
| RC224475L2 | Lenti-ORF clone of NEFM (mGFP-tagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 1 |
CNY 9,030.00 |
|
| RC224475L3 | Lenti-ORF clone of NEFM (Myc-DDK-tagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 1 |
CNY 9,030.00 |
|
| RC224475L4 | Lenti-ORF clone of NEFM (mGFP-tagged)-Human neurofilament, medium polypeptide (NEFM), transcript variant 1 |
CNY 9,030.00 |
|
| RG224475 | NEFM (tGFP-tagged) - Human neurofilament, medium polypeptide (NEFM), transcript variant 1 |
CNY 11,664.00 |
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