NNT (NM_012343) Human Untagged Clone
CAT#: SC108450
NNT (untagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 7,568.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GCCD4 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC108450 sequence for NM_012343 edited (data generated by NextGen Sequencing)
ATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCTACTTAGCAATTTG GGGTCCTGTAAGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATTTTATACTCACCAA GAACTGTGGTGTAAAGCGCCTGTAAAACCAGGAATTCCATATAAGCAACTGACTGTTGGA GTCCCCAAAGAGATATTCCAAAATGAGAAGCGAGTGGCATTGTCTCCTGCTGGTGTTCAG AACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATCGGGTGCGGGCGAAGCTTCCAAG TTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGGGCAAAGGAAGTGCTG GCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAACATTAGGTGTTCAT GAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTTACCCAGCCCAAAAT CCAGAGTTGCTAAATAAACTTTCCCAAAGAAAAACTACAGTTCTGGCAATGGACCAGGTT CCAAGAGTCACAATTGCTCAGGGATATGATGCGCTAAGCTCCATGGCCAACATTGCGGGT TATAAGGCTGTTGTCCTAGCAGCAAATCATTTTGGACGTTTTTTTACTGGTCAGATCACA GCTGCTGGAAAAGTTCCTCCAGCTAAGATTCTGATAGTTGGTGGTGGTGTTGCTGGGCTT GCTTCTGCAGGCGCAGCAAAGTCGATGGGTGCAATTGTTCGAGGATTTGACACAAGAGCT GCAGCTTTGGAACAGTTCAAGTCTCTTGGTGCTGAGCCCTTGGAGGTGGACTTGAAGGAA TCTGGTGAGGGACAAGGAGGATATGCAAAAGAGATGTCCAAAGAGTTCATTGAAGCTGAA ATGAAACTCTTTGCTCAACAATGCAAGGAGGTAGACATCCTTATCAGCACAGCACTTATT CCAGGTAAAAAAGCTCCAGTTTTATTTAATAAAGAAATGATTGAGTCAATGAAGGAAGGT TCAGTTGTTGTGGATTTAGCTGCTGAGGCTGGTGGAAACTTTGAAACCACTAAGCCAGGA GAACTCTACATTCATAAGGGAATTACTCACATAGGCTACACAGACCTGCCCAGCCGAATG GCCACTCAGGCCAGCACCCTATATTCCAACAACATCACCAAACTCCTGAAGGCCATCAGC CCGGACAAAGATAATTTTTATTTTGATGTGAAAGATGACTTTGACTTTGGTACGATGGGT CATGTCATTAGAGGAACTGTAGTGATGAAAGATGGTAAAGTGAWTTTCCCAGCTCCCACA CCGAAAAATATTCCTCAAGGTGCCCCAGTAAAACAGAAGACAGTGGCTGAGCTGGAAGCT GAAAAAGCAGCTACCATTACACCCTTCAGGAAGACAATGTCAACGGCTTCTGCATATACA GCAGGTCTCACAGGGATACTGGGTTTGGGCATTGCGGCTCCCAATCTAGCCTTTTCTCAG ATGGTGACCACTTTTGGCTTGGCTGGCATTGTGGGGTATCATACCGTCTGGGGAGTGACC CCTGCTCTCCACTCACCACTGATGTCTGTGACAAATGCAATCTCAGGGCTGACTGCAGTT GGTGGGTTGGCACTGATGGGAGGACATTTGTATCCTTCCACAACTTCTCAGGGCCTTGCT GCTCTTGCTGCATTCATATCCTCTGTCAACATTGCAGGTGGCTTTCTGGTGACTCAGAGA ATGCTGGACATGTTCAAGCGTCCCACTGACCCCCCAGAATACAACTACCTGTACCTGCTC CCTGCCGGCACCTTTGTTGGTGGATATTTAGCTGCCCTCTACAGTGGTTATAACATTGAA CAGATCATGTACCTAGGCTCGGGTTTGTGCTGTGTCGGTGCCTTGGCTGGCCTCTCCACC CAGGGAACAGCACGTCTTGGCAATGCACTGGGCATGATTGGGGTTGCTGGAGGACTGGCA GCCACCCTCGGAGTCCTAAAACCGGGCCCAGAATTACTAGCTCAGATGTCTGGAGCGATG GCTTTGGGTGGTACCATTGGATTGACAATTGCCAAACGCATCCAGATTTCTGATTTACCT CAATTAGTTGCTGCTTTTCACAGTTTAGTGGGTTTGGCAGCTGTACTTACTTGCATAGCT GAGTACATTATAGAATATCCACATTTTGCTACGGATGCAGCAGCAAATCTCACCAAGATT GTGGCCTACCTCGGCACTTACATTGGTGGCGTCACCTTTAGTGGGTCTCTCATTGCCTAT GGAAAATTGCAGGGTCTCCTGAAATCTGCCCCTCTCCTACTGCCTGGAAGGCACTTACTC AATGCAGGCTTACTGGCTGCTAGTGTGGGCGGGATAATCCCATTCATGGTGGACCCAAGC TTTACTACTGGCATCACCTGTCTGGGTTCAGTGTCTGCTCTCTCTGCTGTCATGGGTGTG ACTTTGACAGCTGCTATTGGGGGTGCTGACATGCCCGTCGTTATCACTGTGCTGAACAGC TACTCAGGCTGGGCCCTGTGTGCAGAGGGCTTCCTGCTCAACAACAATCTGCTGACCATC GTGGGTGCACTCATAGGCTCGTCTGGTGCTATCCTGTCATACATCATGTGTGTGGCAATG AATCGCTCCCTGGCTAATGTGATTCTTGGAGGCTATGGCACCACTTCAACAGCTGGTGGA AAACCCATGGAAATTTCTGGCACACATACGGAAATCAACCTTGACAATGCAATTGACATG ATTCGAGAAGCTAATAGCATTATTATTACACCAGGCTATGGTCTCTGTGCAGCCAAAGCT CAATACCCCATTGCTGATTTGGTAAAGATGCTCACTGAGCAAGGCAAAAAAGTCAGGTTT GGAATTCACCCAGTTGCAGGCCGAATGCCTGGTCAGCTTAATGTGCTGCTGGCTGAGGCT GGTGTGCCATATGACATTGTGTTGGAAATGGATGAGATCAACCATGATTTTCCAGATACT GATTTGGTCCTTGTAATTGGAGCTAATGACACTGTTAATTCAGCAGCTCAAGAAGATCCC AACTCTATTATTGCAGGCATGCCAGTCCTTGAGGTCTGGAAATCAAAGCAGGTGATTGTT ATGAAGAGGTCTTTGGGTGTTGGCTATGCTGCAGTGGACAATCCAATCTTCTACAAACCT AACACGGCCATGCTTCTAGGTGATGCCAAGAAAACATGTGACGCGCTCCAGGCGAAAGTT AGAGAATCCTATCAGAAGTAA Clone variation with respect to NM_012343.3 1304 t=>w >OriGene 5' read for NM_012343 unedited
GGTTTTAGGATTTGTNATCCGACTTCATATAGGGCGGCCGCGCAATTCGCACGAGGGGCA CAGCCCAGGCTGTCAGCCTCCCGGCCCAGTGATTTGCCTTCAAGGAAACTGGGGAGTCAG AAAATTGGGAACTCATATCAACATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTC GTGTCCTCTACTTAGCAATTTGGGGTCCTGTAAGGGTCTACGTGTGAAGAAGGATTTTTT ACGAACATTTTATACTCACCAAGAACTGTGGTGTAAAGCGCCTGTAAAACCAGGAATTCC ATATAAGCAACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAGCGAGTGGC ATTGTCTCCTGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATC GGGTGCGGGCGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAAT CCAAGGGGCAAAGGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGT TAATCCAACATTAGGTGTTCATGAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAG TTTTATTTACCCAGCCCAAAATCCAGAGTTGCTAAATAAACTTTCCCAAAGAAAAACTAC AGTTCTGGCAATGGACCAGGTTCCAAGAGTCACAATTGCTCAGGGATATGATGCGCTAAG CTCCATGGCCAACATTGCGGGTTATAAAGCTGTTGTCCTAGCAGCANATCATTTTGGACG TTTTTTTACTGGTCAGATCACAGCTGCTGGGAAAGTTCCTCCAGCTAAGATCTGAAGTTG GTGGTGGGTGTTGCTGGGCTTGCTTCTGCAGCGCAGCANATCGAGGGTGCAATTGTCGAG GATTGACACAGACTGCAGCTTGGAACAGTTC >OriGene 3' read for NM_012343 unedited
CCTTCGTTCTATTTCCCCACCTTCCACACCAGGNTTTGTGTGNANGAAAACCGCAATCCG AGGCCNGGTTTTTTTCCTTTTTTTTTTTTTGCCAACTGTTTTACTCTTTTATAGCCAAAG TTGAATTCCACTCAAGTGCACTTAATATAGTACTACACATTATAAGTTGATCGAAATTAA AGAGATCTCCAATTAGAAATGGGATCTTCAAAAATACTATTACTAATTTTCAAGCTGGAT GTTTCATTTTTTTTCTAATGAAACATATAAAGCAAATGATTTGTTCCCAGCCTCTCTGAA AGATTATTTAGCCCTTTAATGTATTAAAATAGTAGTGAGAAAATGTGCCCTTGAAATATA GACTTTGAAGTACAATTTAAAAAATTAAAACTGACTTGATATGATTAATTCAAACTGCGG TTGAATTGCAAGGGGACAGCTCATAAGCTCATAAAAGGTTCAAATTAGCCACATTTGTTG AAAAAAAAAACATAAAATGCAGGTGCCTGCAATGGTTACATTGTGTCAGATATTTCAGAA GCATGTATAACGCTTGCCTTGAAATCCTAAACACTTACCTTTTTTAAACATTTTTTGGCT ACTGAGGAGGTATCAAAAATTCAACAGGACTACCTCTAACATGACGGCTGTTCTTTCTCC CTTTATTCAACACCTTTTGACTTCCTACACAAAAAAAATATGGCCTAGAAAGACATTTAG AATCTTTCCATTCCTTTTTAAAAATTGAGGGATTACCGTATTTTGAAAATTTTTTTCTAT ACCGCTCTTTGCCTTTGCTTTTTTCCACCCTTTATTTCCTTCCACAAGTTCTCTTTCCCA ATGGTCATTCCCCTTGTTTACGGGACCTCTCCTCGCGTTGTTCCCAAACATTGCAAGGTG GGTCTCTTAACTTAACGCTGGGTCCTCTATAATAAATCCCGGGAGGAATTTGTTAATCCC CGCCCCGAAAGGGTAAAAT |
Restriction Sites | NotI-NotI |
ACCN | NM_012343 |
Insert Size | 4620 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012343.2, NP_036475.2 |
RefSeq Size | 4600 bp |
RefSeq ORF | 3261 bp |
Locus ID | 23530 |
UniProt ID | Q13423 |
Domains | PNTB, AlaDh_PNT_C, AlaDh_PNT_N |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism |
Gene Summary | This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224002 | NNT (Myc-DDK-tagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 7,560.00 |
|
RC224002L1 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 9,960.00 |
|
RC224002L2 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 10,260.00 |
|
RC224002L3 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 10,260.00 |
|
RC224002L4 | Lenti ORF clone of Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 10,260.00 |
|
RG224002 | NNT (tGFP-tagged) - Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 9,160.00 |