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EIF3B (NM_003751) Human Untagged Clone
CAT＃: SC108421
EIF3B (untagged)-Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1
CNY 6,984.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; PRT1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003751, the custom clone sequence may differ by one or more nucleotides
ATGCAGGACGCGGAGAACGTGGCGGTGCCCGAGGCGGCCGAGGAGCGCGCCGAGCCCGGCCAGCAGCAGC CGGCCGCCGAGCCGCCGCCAGCCGAGGGGCTGCTGCGGCCCGCGGGGCCCGGCGCTCCGGAGGCCGCGGG GACCGAGGCCTCCAGTGAGGAGGTGGGGATCGCGGAGGCCGGGCCGGAGTCCGAGGTGAGGACCGAGCCG GCGGCCGAGGCAGAGGCGGCCTCCGGCCCGTCCGAGTCGCCCTCGCCGCCGGCCGCCGAGGAGCTGCCCG GGTCGCATGCTGAGCCCCCTGTCCCGGCACAGGGCGAGGCCCCAGGAGAGCAGGCTCGGGACGAGCGCTC CGACAGCCGGGCCCAGGCGGTGTCCGAGGACGCGGGAGGAAACGAGGGCAGAGCGGCCGAGGCCGAACCC CGGGCGCTGGAGAACGGCGACGCGGACGAGCCCTCCTTCAGCGACCCCGAGGACTTCGTGGACGACGTGA GCGAGGAAGAATTACTGGGAGATGTACTCAAAGATCGGCCCCAGGAAGCAGATGGAATCGATTCGGTGAT TGTAGTGGACAATGTCCCTCAGGTGGGACCCGACCGACTTGAGAAACTCAAAAATGTCATCCACAAGATC TTTTCCAAGTTTGGGAAAATCACAAATGATTTTTATCCTGAAGAGGATGGGAAGACAAAAGGGTATATTT TCCTGGAGTACGCGTCCCCTGCCCACGCTGTGGATGCTGTGAAGAACGCCGACGGCTACAAGCTTGACAA GCAGCACACATTCCGGGTCAACCTCTTTACGGATTTTGACAAGTATATGACGATCAGTGACGAGTGGGAT ATTCCAGAGAAACAGCCTTTCAAAGACCTGGGGAACTTACGTTACTGGCTTGAAGAGGCAGAATGCAGAG ATCAGTACAGTGTGATTTTTGAGAGTGGAGACCGCACTTCCATATTCTGGAATGACGTAAAAGACCCTGT CTCAATTGAAGAAAGAGCGAGATGGACAGAGACGTATGTGCGTTGGTCTCCTAAGGGCACCTACCTGGCT ACCTTTCATCAAAGAGGCATTGCTCTATGGGGGGGAGAGAAATTCAAGCAAATTCAGAGATTCAGCCACC AAGGGGTTCAGCTTATTGACTTCTCACCTTGTGAAAGGTACCTGGTGACCTTTAGCCCCCTGATGGACAC GCAGGATGACCCTCAGGCCATAATCATCTGGGACATCCTTACGGGGCACAAGAAGAGGGGTTTTCACTGT GAGAGCTCAGCCCATTGGCCTATTTTTAAGTGGAGCCATGATGGCAAATTCTTTGCCAGAATGACCCTGG ATACGCTTAGCATCTATGAAACTCCTTCTATGGGTCTTTTGGACAAGAAGAGTTTGAAGATCTCTGGGAT AAAAGACTTTTCTTGGTCTCCTGGTGGTAACATAATCGCCTTCTGGGTGCCTGAAGACAAAGATATTCCA GCCAGGGTAACCCTGATGCAGCTCCCTACCAGGCAAGAGATCCGAGTGAGGAACCTGTTCAATGTGGTGG ACTGCAAGCTCCATTGGCAGAAGAACGGAGACTACTTGTGTGTGAAAGTAGATAGGACTCCGAAAGGCAC CCAGGGTGTTGTCACAAATTTTGAAATTTTCCGAATGAGGGAGAAACAGGTACCTGTGGATGTGGTCGAG ATGAAAGAAACCATCATAGCCTTTGCCTGGGAACCAAATGGAAGTAAGTTTGCTGTGCTGCACGGAGAGG CTCCGCGGATATCTGTGTCTTTCTACCACGTCAAAAACAACGGGAAGATTGAACTCATCAAGATGTTCGA CAAGCAGCAGGCGAACACCATCTTCTGGAGCCCCCAAGGACAGTTCGTGGTGTTGGCGGGCCTGAGGAGT ATGAACGGTGCCTTAGCGTTTGTGGACACTTCGGACTGCACGGTCATGAACATCGCAGAGCACTACATGG CTTCCGACGTCGAATGGGATCCTACTGGGCGCTACGTCGTCACCTCTGTGTCCTGGTGGAGCCATAAGGT GGACAACGCGTACTGGCTGTGGACTTTCCAGGGACGCCTCCTGCAGAAGAACAACAAGGACCGCTTCTGC CAGCTGCTGTGGCGGCCCCGGCCTCCCACACTCCTGAGCCAGGAACAGATCAAGCAAATTAAAAAGGATC TGAAGAAATACTCTAAGATCTTTGAACAGAAGGATCGTTTGAGTCAGTCCAAAGCCTCAAAGGAATTGGT GGAGAGAAGGCGCACCATGATGGAAGATTTCCGGAAGTACCGGAAAATGGCCCAGGAGCTCTATATGGAG CAGAAAAACGAGCGCCTGGAGTTGCGAGGAGGGGTGGACACTGACGAGCTGGACAGCAACGTGGACGACT GGGAAGAGGAGACCATTGAGTTCTTCGTCACTGAAGAAATCATTCCCCTCGGGAATCAGGAGTGA >OriGene 5' read for NM_003751 unedited
CTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGCGCGGCGGCCGCGGAGCCCTGCGA GTAGGCAGCGTTGGGCCCATGCAGGACGCGGAGAACGTGGCGGTGCCCGAGGCGGCCGAG GAGCGCGCCGAGCCCGGCCAGCAGCAGCCGGCCGCCGAGCCGCCGCCAGCCGAGGGGCTG CTGCGGCCCGCGGGGCCCGGCGCTCCGGAGGCCGCGGGGACCGAGGCCTCCAGTGAGGAG GTGGGGATCGCGGAGGCCGGGCCGGAGCCCGAGGTGAGGACCGAGCCGGCGGCCGAGGCA GAGGCGGTCTCCGGCCCGTCCGAGTCGCCCTCGCCGCCGGCCGCCGAGGAGCTGCCCGGG TCGCATGCTGAGCCCCCTGTCCCGGCACAGGGCGAGGCCNCAGGAGAGCAGGCTCGGGAC GAGCGCTCCGACAGCCGGGCCCAGGCGGTGTCCGAGGACGCGGGAGGAAACGAGGGCAGA GCGGCCGAGGCCGAACCACGGACGCTGGAGAACGGCGACGCNGACGAGCCCTNCTTCATC GACCCCCGAGACTTCGTGGACGACGTGAGCGAGGAAGGAATACTGGAGATGTACTCAGAG ATCCGGCCCAGGAATCAGATGGAACTCGATCGGTGATTGCAGTGGACAATGTCCCTCATG TGGGTACCCGACCGACTCGAGAAACTCAGAAATGTCATCCACATGATCCTTTCCAAGCTT GCGACAATCACAAATGATTTTTATGCTGAAGAGGATGGGAAGACAAAAGGGTCTATGTTA CTGGAGTACGCGTCCCCTGCCACCGCTGAGATGCTGTGAATACGCCGACGCNTACAGCCT GACAGCACACACATTCCGGTCAACCTCTTACGGTTTGACAGTTATGACGATCGTGACAGT GGGATCTGCAAGGACAGCTTTAAGACTGGGGACTACGTACTGCTTGAGAGCNTAAGCNGA ATCATCAGGGGATTTTTAAGGGAAACGGCTCCTACTCGG >OriGene 3' read for NM_003751 unedited
CCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAATACAAGTTTGCTTTTATTTCCA TGTTCAACCTGCGGAGAAAACCGGCCACAACCTGCACGGTGGGGGTACAAGGCATGGCGG CGCCCTCCCGGTGGGTCTCCGGGACACGTCCAGCACTGCCCGCAATGGGGATGCAAGTCC TTCAACGGAAATGCTGCTCGGCTGTGTACAGGCACCGTGGAAACATCACTGGGCGCAGGA GAAGGTGCCGCCAGTGGCGTCGCTAAGTCTTCGGAGCCAACGCCGGCGGTTCCAACAGAA AACTCCAAACAAGAAAGAAGTGGAAGCGGGTGCCTTAAATCCCCCACTGCAGACACCAGG GGCTGAAAGGCACAAAAATGTGTCGCAATGGCAGAATCCAGGCGCAGTCACAGTCCAGAG CCAGAGAGCACAGGGAGGAGGCGGGAGTCACGCGGCTTCCTGCAGGACGGCCTCGGCTCC ACAGCACACACGGCACAGTGCTCCAGGTCACTCCTGATTCCCGAGGGGAATGATTTCTTC AGTGACGAAGAACTCAATGGTCTCCTCTTCCCAGTCGTCCACGTTGCTGTCCAGCTCGTC AGTGTCCACCCCTCCTCGCAACTCCAGGCGCTCGTTGTTCTGCTCCATATAGAGCTCCTG GGCCTTTTTCCGGTACTTCCGGAAATCTTCCATCATGGGGCGCCTTCTCTCCACCATTCC CTTGGAGGCTTTGGACTGACTCAAACGATCCTTCTGTTCAAAGATCTAGAGTATTCTTTC AGAACCTTTTCAATATGCTCGAACCGGTCCCTGGCTCAAGAGTGTGGGAAGCCGGGGCCC CCCCACCATCTGGCATAACCGTCCCTGATGTTCTTTTGCAGAAGGGTCCTGAAAGGTCCA AACATCACCGTGGCCACTCATGCTTCCCAAAACAAAGGGACACTTACCCCAATAGAACCC TCCACCCGGAACCTTTTGCCTCGCAGGTATACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003751 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003751.2, NP_003742.2 |
| RefSeq Size | 3009 bp |
| RefSeq ORF | 3009 bp |
| Locus ID | 8662 |
| UniProt ID | P55884 |
| Domains | RRM |
| Gene Summary | RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 3' UTR compared to variant 2. Variants 1, 2 and 3 encode the same isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211093 | EIF3B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1 |
CNY 5,960.00 |
|
| RC211093L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211093L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG211093 | EIF3B (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1 |
CNY 4,370.00 |
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