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UBE2I (NM_194259) Human Untagged Clone
CAT＃: SC107675
UBE2I (untagged)-Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 2
CNY 1,200.00
CNY 2,950.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C358B7.1; P18; UBC9 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_194259 edited
ATGTCGGGGATCGCCCTCAGCAGACTCGCCCAGGAGAGGAAAGCATGGAGGAAAGACCAC CCATTTGGTTTCGTGGCTGTCCCAACAAAAAATCCCGATGGCACGATGAACCTCATGAAC TGGGAGTGCGCCATTCCAGGAAAGAAAGGGACTCCGTGGGAAGGAGGCTTGTTTAAACTA CGGATGCTTTTCAAAGATGATTATCCATCTTCGCCACCAAAATGTAAATTCGAACCACCA TTATTTCACCCGAATGTGTACCCTTCGGGGACAGTGTGCCTGTCCATCTTAGAGGAGGAC AAGGACTGGAGGCCAGCCATCACAATCAAACAGATCCTATTAGGAATACAGGAACTTCTA AATGAACCAAATATCCAAGACCCAGCTCAAGCAGAGGCCTACACGATTTACTGCCAAAAC AGAGTGGAGTACGAGAAAAGGGTCCGAGCACAAGCCAAGAAGTTTGCGCCCTCATAA >OriGene 5' read for NM_194259 unedited
CACAATTTCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC CTGCGAATTCGGCACGAGGTTTGAACATGTCGGGGATCGCCCTCAGCAGACTCGCCCAGG AGAGGAAAGCATGGCAGGAAAGACCACCCATTTGGTTTCGTGGCTGTCCCAACAAAAAAT CCCGATGGCACGATGAACCTCATGAACTGGGAGTGCGCCATTCCAGGAAAGAAAGGGACT CCGTGGGAAGGAGGCTTGTTTAAACTACGGATGCTTTTCAAAGATGATTATCCATCTTCG CCACCAAAATGTAAATTCGAACCACCATTATTTCACCCGAATGTGTACCCTTCGGGGACA GTGTGCCTGTCCATCTTAGAGGAGGACAAGGACTGGAGGCCAGCCATCACAATCAAACAG ATCCTATTAGGAATACAGGAACTTCTAAATGAACCAAATATCCAAGACCCAGCTCAAGCA GAGGCCTACACGATTTACTGCCAAAACAGAGTGGAGTACGAGAAAAGGGTCCGAGCACAA GCCAAGAAGTTTGCGCCCTCATAAGCAGCGACCTTGTGGCATCGTCAGACGGACGGGATT GGTTTGGCACGAACTTGTTTACAACATTTTTGCAAATCTAAAGTTGCTCCATACAATGAC TAGTCACCTGNGGGGGGATGGGCGGGCGCCATCTTCCATTGCCGCCGCGGATGTGCGGTC TCGAATCGCTGAATTGCCCGTTTCATACAGGGACTCTTCCTTCGGACTTTTGAATATTTG ACTGGTATGCAAACTCGCTCTTATATTATATTGATGTCAGTATTTCACTGCTGTAAACTA TAACTTTATACTGGGTAGTCCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_194259 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_194259.1, NP_919235.1 |
| RefSeq Size | 1478 bp |
| RefSeq ORF | 477 bp |
| Locus ID | 7329 |
| UniProt ID | P63279 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) has an additional exon in the 5' UTR, as compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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SUMOylation Regulates Insulin Exocytosis Downstream of Secretory Granule Docking in Rodents and Humans
,Xiao-Qing Dai, Greg Plummer, Marina Casimir, Youhou Kang, Catherine Hajmrle, Herbert Y. Gaisano, Jocelyn E. Manning Fox, and Patrick E. MacDonald,
Diabetes, Mar 2011; 60: 838 - 847
[UBE2I]
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SUMOylation regulates Kv2.1 and modulates pancreatic -cell excitability
,Xiao-Qing Dai, Jelena Kolic, Paolo Marchi, Simonetta Sipione, and Patrick E. MacDonald,
J. Cell Sci., Feb 2009; 10.1242/jcs.036632.
[UBE2I]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217945 | UBE2I (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 2 |
CNY 1,200.00 |
|
| RC217945L1 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 2, Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC217945L2 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC217945L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217945L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG217945 | UBE2I (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 2 |
CNY 2,800.00 |
