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PPP2R2B (NM_181674) Human Untagged Clone
CAT＃: SC107323
PPP2R2B (untagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B; PR55-BETA; PR55BETA; SCA12 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_181674, the custom clone sequence may differ by one or more nucleotides
ATGCTTCTCTCGTTACCTGCCTTACATCTTCAGACCTCCGAACACCATCCTTTCTTCCAGCTGCCACACA GAAGGCTCGGACCATGGTGCAGTCCCACTGGCTCCCCTGCCCCCCTCTCCTGTGAGACTGGCTGCGGGGA GGGATCATGGATACTTGTCTGCCGGCTTCTGGTTCCCACGCAAGTAAGCCTGCTGTCAATGGAGGAGGAC ATTGATACCCGCAAAATCAACAACAGTTTCCTGCGCGACCACAGCTATGCGACCGAAGCTGACATTATCT CTACGGTAGAATTCAACCACACGGGAGAATTACTAGCGACAGGGGACAAGGGGGGTCGGGTTGTAATATT TCAACGAGAGCAGGAGAGTAAAAATCAGGTTCATCGTAGGGGTGAATACAATGTTTACAGCACATTCCAG AGCCATGAACCCGAGTTCGATTACCTGAAGAGTTTAGAAATAGAAGAAAAAATCAATAAAATAAGATGGC TCCCCCAGCAGAATGCAGCTTACTTTCTTCTGTCTACTAATGATAAAACTGTGAAGCTGTGGAAAGTCAG CGAGCGTGATAAGAGGCCAGAAGGCTACAATCTGAAAGATGAGGAGGGCCGGCTCCGGGATCCTGCCACC ATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTGGAGGCCACCCCACGAAGAGTAT TTGCCAACGCACACACATATCACATCAACTCCATATCTGTCAACAGCGACTATGAAACCTACATGTCCGC TGATGACCTGAGGATTAACCTATGGAACTTTGAAATAACCAATCAAAGTTTTAATATTGTGGACATTAAG CCAGCCAACATGGAGGAGCTCACGGAGGTGATCACAGCAGCCGAGTTCCACCCCCATCATTGCAACACCT TCGTGTACAGCAGCAGCAAAGGGACAATCCGGCTGTGTGACATGCGGGCATCTGCCCTGTGTGACAGGCA CACCAAATTTTTTGAAGAGCCGGAAGATCCAAGCAACAGATCATTTTTCTCTGAAATTATCTCTTCGATT TCGGATGTGAAGTTCAGCCACAGTGGGAGGTATATCATGACCAGGGACTACTTGACCGTCAAAGTCTGGG ATCTCAACATGGAAAACCGCCCCATCGAGACTTACCAGGTTCATGACTACCTCCGCAGCAAGCTGTGTTC CCTCTATGAAAATGACTGCATTTTTGATAAATTTGAGTGTGTGTGGAATGGGTCAGACAGTGTCATCATG ACAGGCTCCTACAACAACTTCTTCAGGATGTTCGACAGAAACACCAAGCGTGATGTGACCCTTGAGGCTT CGAGGGAAAACAGCAAGCCCCGGGCTATCCTCAAACCCCGAAAAGTGTGTGTGGGGGGCAAGCGGAGAAA AGACGAGATCAGTGTCGACAGTCTGGACTTTAGCAAAAAGATCTTGCATACAGCTTGGCATCCTTCAGAA AATATTATAGCAGTGGCGGCTACAAATAACCTATATATATTCCAGGACAAGGTTAACTAG >OriGene 5' read for NM_181674 unedited
GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCGCTGATGGCA AAATGGGACCCATCTCCAGTTATTCCTCGGGCACCCCAGCTTTGCAAATCTGGGAAAGAA CCTACATAAACATATTGGTGATGTAGCCAGCTTAAAAGGCCGAGAGAATCCTTATGGATT CAGGAGACTGTTTTTTTATTTAGATTTTTATTTTTTATTTGGAAGATGAAATGCTTCTCT CGTTACCTGCCTTACATCTTCAGACCTCCGAACACCATCCTTTCTTCCAGCTGCCACACA GAAGGCTCGGACCATGGTGCAGTCCCACTGGCTCCCCTGCCCCCCTCTCCTGTGAGACTG GCTGCGGGGAGGGATCATGGATACTTGTCTGCCGGCTTCTGGTTCCCACGCAAGTAAGCC TGCTGTCAATGGAGGAGGACATTGATACCCGCAAAATCAACAACAGTTTCCTGCGCGACC ACAGCTATGCGACCGAAGCTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAAT TACTAGCGACAGGGGACAAGGGGGGTCGGGTTGTAATATTTCAACGAGAGCAGGAGAGTA AAAATCAGGTTCATCGTAGGGGTGAATACAATGTTTACAGCACATTCCAGAGCCATGAAC CCCGAGTTCGATTACCTCGAAGAGGTTTAGAAATAGAAGAGAAAATCAATAGAATAAGAT GGCCTCCCCCAGCAGAATGCAGCTTACTTTCTTCTGTCTACTAATGATAANNACTGTGAA CTGTGGGAAAGTCAGCGAGCGTGATTNAGAGCCCAGAAAGCTACATCTGAAGATAAAAAG AGGGCCGCTCTCCGGTATCTGCCACATACACCCTTGCGGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_181674 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181674.1, NP_858060.1 |
| RefSeq Size | 2284 bp |
| RefSeq ORF | 1332 bp |
| Locus ID | 5521 |
| UniProt ID | Q00005 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Tight junction |
| Gene Summary | The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region, compared to variant 3. The resulting isoform (a) is shorter and has a distinct N-terminus, compared to isoform e. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224533 | PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2 |
CNY 3,656.00 |
|
| RC224533L3 | Lenti-ORF clone of PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2 |
CNY 5,890.00 |
|
| RC224533L4 | Lenti-ORF clone of PPP2R2B (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2 |
CNY 5,890.00 |
|
| RG224533 | PPP2R2B (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2 |
CNY 4,370.00 |
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