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GPAM (NM_020918) Human Untagged Clone
CAT＃: SC106982
GPAM (untagged)-Human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), nuclear gene encoding mitochondrial protein
CNY 6,072.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPAT; GPAT1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_020918 edited
GAATTCGGCACGAGGAGTGCGGGCTTCTGCAGCAGCCGAAGCTGGAGCTGCTAGGGCAGC AGCGGCTCCCCTGTTGTATGGACATTCTGCACCCGAAACTGATAGCTGAGTCCTGAAGTT TTATGTTATGAAACAGAAGAACTTTCATCCCAGCACATGATTTGGGAATTACACTTTGTG ACATGGATGAATCTGCACTGACCCTTGGTACAATAGATGTTTCTTATCTGCCACATTCAT CAGAATACAGTGTTGGTCGATGTAAGCACACAAGTGAGGAATGGGGTGAGTGTGGCTTTA GACCCACCGTCTTCAGATCTGCAACTTTAAAATGGAAAGAAAGCCTAATGAGTCGGAAAA GGCCATTTGTTGGAAGATGTTGTTACTCCTGCACTCCCCAGAGCTGGGACAAATTTTTCA ACCCCAGTATCCCGTCTTTGGGTTTGCGGAATGTTATTTATATCAATGAAACTCACACAA GACACCGCGGATGGCTTGCAAGACGCCTTTCTTACGTTCTTTTTATTCAAGAGCGAGATG TGCATAAGGGCATGTTTGCCACCAATGTGACTGAAAATGTGCTGAACAGCAGTAGAGTAC AAGAGGCAATTGCAGAAGTGGCTGCTGAATTAAACCCTGATGGTTCTGCCCAGCAGCAAT CAAAAGCCGTTAACAAAGTGAAAAAGAAAGCTAAAAGGATTCTTCAAGAAATGGTTGCCA CTGTCTCACCGGCAATGATCAGACTGACTGGGTGGGTGCTGCTAAAACTGTTCAACAGCT TCTTTTGGAACATTCAAATTCACAAAGGTCAACTTGAGATGGTTAAAGCTGCAACTGAGA CGAATTTGCCGCTTCTGTTTCTACCAGTTCATAGATCCCATATTGACTATCTGCTGCTCA CTTTCATTCTCTTCTGCCATAACATCAAAGCACCATACATTGCTTCAGGCAATAATCTCA ACATCCCAATCTTCAGTACCTTGATCCATAAGCTTGGGGGCTTCTTCATACGACGAAGGC TCGATGAAACACCAGATGGACGGAAAGATGTTCTCTATAGAGCTTTGCTCCATGGGCATA TAGTTGAATTACTTCGACAGCAGCAATTCTTGGAGATCTTCCTGGAAGGCACACGTTCTA GGAGTGGAAAAACCTCTTGTGCTCGGGCAGGACTTTTGTCAGTTGTGGTAGATACTCTGT CTACCAATGTCATCCCAGACATCTTGATAATACCTGTTGGAATCTCCTATGATCGCATTA TCGAAGGTCACTACAATGGTGAACAACTGGGCAAACCTAAGAAGAATGAGAGCCTGTGGA GTGTAGCAAGAGGTGTTATTAGAATGTTACGAAAAAACTATGGTTGTGTCCGAGTGGATT TTGCACAGCCATTTTCCTTAAAGGAATATTTAGAAAGCCAAAGTCAGAAACCGGTGTCTG CTCTACTTTCCCTGGAGCAAGCGTTGTTACCAGCTATACTTCCTTCAAGACCCAGTGATG CTGCTGATGAAGGTAGAGACACGTCCATTAATGAGTCCAGAAATGCAACAGATGAATCCC TACGAAGGAGGTTGATTGCAAATCTGGCTGAGCATATTCTATTCACTGCTAGCAAGTCCT GTGCCATTATGTCCACACACATTGTGGCTTGCCTGCTCCTCTACAGACACAGGCAGGGAA TTGATCTCTCCACATTGGTCGAAGACTTCTTTGTGATGAAAGAGGAAGTCCTGGCTCGTG ATTTTGACCTGGGGTTCTCAGGAAATTCAGAAGATGTAGTAATGCATGCCATACAGCTGC TGGGAAATTGTGTCACAATCACCCACACTAGCAGGAACGATGAGTTTTTTATCACCCCCA GCACAACTGTCCCATCAGTCTTCGAACTCAACTTCTACAGCAATGGGGTACTTCATGTCT TTATCATGGAGGCCATCATAGCTTGCAGCCTTTATGCAGTTCTGAACAAGAGGGGACTGG GGGGTCCCACTAGCACCCCACCTAACCTGATCAGCCAGGAGCAGCTGGTGCGGAAGGCGG CCAGCCTGTGCTACCTTCTCTCCAATGAAGGCACCATCTCACTGCCTTGCCAGACATTTT ACCAAGTCTGCCATGAAACAGTAGGAAAGTTTATCCAGTATGGCATTCTTACAGTGGCAG AGCACGATGACCAGGAAGATATCAGTCCTAGTCTTGCTGAGCAGCAGTGGGACAAGAAGC TTCCTGAACCTTTGTCTTGGAGAAGTGATGAAGAAGATGAAGACAGTGACTTTGGGGAGG AACAGCGAGATTGCTACCTGAAGGTGAGCCAATCCAAGGAGCACCAGCAGTTTATCACCT TCTTACAGAGACTCCTTGGGCCTTTGCTGGAGGCCTACAGCTCTGCTGCCATCTTTGTTC ACAACTTCAGTGGTCCTGTTCCAGAACCTGAGTATCTGCAAAAGTTGCACAAATACCTAA TAACCAGAACAGAAAGAAATGTTGCAGTATATGCTGAGAGTGCCACATATTGTCTTGTGA AGAATGCTGTGAAAATGTTTAAGGATATTGGGGTTTTCAAGGAGACCAAACAAAAGAGAG TGTCTGTTTTAGAACTGAGCAGCACTTTTCTACCTCAATGCAACCGACAAAAACTTCTAG AATATATTCTGAGTTTTGTGGTGCTGTAGGTAACGTGTGGCACTGCTGGCAAATGAAGGT CATGAGATGAGTTCCTTGTAGGTACCAGCTTCTGGCTCAAGAGTTGAAGGTGCCGTCGCA GGGTCAGGCCTGCCCTGTCCCGAGGTGATCTCCTGGAAGACAAGTGCCTTCTCCCTCCAT GGATCTGTGATCTTCCCAGCTCTGCATCAACACAGCAGCCTGCAGATAACACTTGGGGGG ACCTCAGCCTCTATTCGCAACTCATAATCCGTAGACTACAAGATGAAATCTCAATAAATT ATTTTTGAGTTTATTAAAGATTGACATTTTAAGTACAACGTTTAAGGACTAATTACTGTG ATGGACACAGAAATGTAGCTGTGTTCTGGAACTG >OriGene 5' read for NM_020918 unedited
ATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGAGTGCGGGCTTC TGCAGCAGCCGAAGCTGGAGCTGCTAGGGCAGCAGCGGCTCCCCTGTTGTATGGACATTC TGCACCCGAAACTGATAGCTGAGTCCTGAAGTTTTATGTTATGAAACAGAAGAACTTTCA TCCCAGCACATGATTTGGGAATTACACTTTGTGACATGGATGAATCTGCACTGACCCTTG GTACAATAGATGTTTCTTATCTGCCACATTCATCAGAATACAGTGTTGGTCGATGTAAGC ACACAAGTGAGGAATGGGGTGAGTGTGGCTTTAGACCCACCGTCTTCAGATCTGCAACTT TAAAATGGAAAGAAAGCCTAATGAGTCGGAAAAGGCCATTTGTTGGAAGATGTTGTTACT CCTGCACTCCCCAGAGCTGGGACAAATTTTTCAACCCCAGTATCCCGTCTTTGGGTTTGC GGAATGTTATTTATATCAATGAAACTCACACAAGACACCGCGGATGGCTTGCAAGACGCC TTTCTTACGTTCTTTTTATTCAAGAGCGAGATGTGCATAAGGGCATGTTTGCCACCAATG TGACTGAAAATGTGCTGAACAGCAGTAGAGTACAAGAGGCAATTGCAGAAGTGGCTGCTG AATTAAACCCTGATGGTTCTGCCCAGCAGCAATCAAAAGCCGTTAACAAAGTGAAAAAGA AAGCTAAAAGGATTCTTCAAGAAATGGTTGCCACTGTCTCACCGGGCATGATCAGACTGA CTGGGTGGGTGCTGCTAAAACTGTTCAACAGCTTCTTTTGGACATCAAATTCACAAAGGT CAAACTTGAGATGTTAAAG >OriGene 3' read for NM_020918 unedited
CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAACTTGACCACAGTCTT CCCCTGAAGAAGGGCATGCATTTCATGTTACAGAAATGCGATAGAGTAAACTGTAATAAA TTATTTACAAGCACCTAGTTTGTTTAACCTTATGTGGAAGCCATCACTGTTGGAAAACAA TGAGAATGTATCTTTTAGCAAAACGGTGCTATCTGGAAGCTTCATTGTGAAGATGCTTTC GTTTTTTTGTTTTTTGTTTTCAGTCATGAGGCACTGAAGAATGAGTTGTGCTGAAATTAA CTCAAAGGTCAGCTTGTCTGGATGAGCATAACTTTGGTTGAGATTTTTCTCCCTTAAAAA AGATTTTCAAAGCACCAGTTAATTACAAAAAGCATGCATATTTATCCTCACAGTGAGTTA AGTGGTGAGAGAGCTAGCAAATCATACATTGCATTCCCCAAAGCATCTGAACGTACTTCT AGAAAACAAACCAACCAAAAGGGAAAATAATGCAAGAGAAGCCGTATTTTCTTTGCTTAG GTTGGCAAAGCAGCAGCTCTTTGCAGCAATGACAGGCAGGGCAGAGTGTCGACTGGGAAG CGAGTCCCAATCTTGAATAATGGTGAGCTTGAGTAATCCTTAATTTACAATTTAAAGGAT GAAAAAAAGAGACTAGTGAAATGTTTGCTTCCCCAGATAGGAACACCCAAGACTCCCTGG GTATCCTCCAAATGTAGCCAAGAAAAGTGNTCTTCTACCCTAGTCAGTAAAATGGGATGC TAAAACATGATATCTCATTCACATCAGAGCTACTACAACTGACAATGACTTCATGGGATA CATCAATTCCTATAAGAAAAAATATATTNTAAATGCAGAACTTGAGAGGGATCACACAGC ATAGCATTATTGCTATGAGTTTCAAACATGGGATTTCATACAATGTGAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020918 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020918.2, NP_065969.2 |
| RefSeq Size | 6387 bp |
| RefSeq ORF | 2487 bp |
| Locus ID | 57678 |
| UniProt ID | Q9HCL2 |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways |
| Gene Summary | This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The microsomal cardiolipin remodeling enzyme acyl-CoA lysocardiolipin acyltransferase is an acyltransferase of multiple anionic lysophospholipids
,Yang Zhao, Yan-Qun Chen, Shuyu Li, Robert J. Konrad, and Guoqing Cao,
J. Lipid Res., May 2009; 50: 945 - 956.
[GPAM]
|
|
Distinct effects of N-acetylgalactosamine-4-sulfatase and galactose-6-sulfatase expression on chondroitin sulfates
,Sumit Bhattacharyya, Kumar Kotlo, Sagar Shukla, Robert S. Danziger, and Joanne K. Tobacman,
J. Biol. Chem. 2008; 283(15): p. 9523-9530
[GPAM]
|
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Identification and characterization of a lysophosphatidylcholine acyltransferase that is primarily expressed in metabolic tissues Yang Zhao, YanQun Chen, Tabetha M. Bonacci, David S. Bredt, Shuyu Li, William R. Bensch, David E. Moller, Mark Kowala, Robert J. Konrad, and Guoqing Cao
,Yang Zhao, YanQun Chen, Tabetha M. Bonacci, David S. Bredt, Shuyu Li, William R. Bensch, David E. Moller, Mark Kowala, Robert J. Konrad, and Guoqing Cao,
J. Biol. Chem., Jan 2008; 10.1074/jbc.M710422200.
[GPAM]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205509 | GPAM (Myc-DDK-tagged)-Human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), nuclear gene encoding mitochondrial protein |
CNY 6,064.00 |
|
| RC205509L1 | Lenti ORF clone of Human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 8,464.00 |
|
| RC205509L2 | Lenti ORF clone of Human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 7,700.00 |
|
| RC205509L3 | Lenti ORF clone of Human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC205509L4 | Lenti ORF clone of Human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 7,700.00 |
|
| RG205509 | GPAM (tGFP-tagged) - Human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), nuclear gene encoding mitochondrial protein |
CNY 7,664.00 |
