C12orf51 (BC006270) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AF-1 specific protein phosphatase; chromosome 12 open reading frame 51; DKFZp586O1022; FLJ10510; FLJ30092; FLJ30208; FLJ34154; KIAA0614; MGC126531
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC006270, the custom clone sequence may differ by one or more nucleotides GACCTCCTTCCGGAAGACGCTGGTGGGCGAGCCCTTGGACCCTGAGCAAGACCTGCAGGAAGCGGATATC CTCACCTACAATTACGTCAAGAAGTTTGAGAGCATCAATGATGAGACCGAGCTGGAGGCCCTGTGCGCTG AGATCGCCTCCCAGCACCTGGCCACGGAGAGCCCTGACAGCCCCAACAAGCCCTGCTGCAGGTTCACCTA CCTGACCATGACGGGCGAGGAGGTGGAGCTGTGCAGCCGGGGCCGGCACATCCTTGTGGCGTGGGAGAAC AAGGACATCTACGCGGCAGCCATCCGGAGCCTGCGGCTGCGGGAGCTGCAGAATGTGGAGTGCGTGACGG CCGTGCGGGCCGGCCTGGGCTCCATCATCCCCCTGCAGCTGCTGACCATGCTCAGCCCACTGGAGATGGA GCTGCGCACCTGCGGCCTCCCCTACATCAACCTCGAGTTCCTCAAGGCCCACACCATGTACCAAGTGGGG CTGATGGAGACGGACCAGCACATCGAGTTCTTCTGGGGGGCCCTGGAGATGTTCACCCAGGAGGAGCTGT GCAAGTTCATCAAGTTTGCCTGCAACCAGGAGCGCATCCCGTTCACCTGCCCCTGCAAAGATGGGGGTCC CGACACTGCCCATGTGCCCCCGTACCCCATGAAGATCGCCCCCCCAGATGGCACAGCAGGTTCCCCAGAC TCTCGCTACATCCGCGTGGAGACCTGCATGTTCATGATCAAGCTTCCCCAGTACTCCTCTCTGGAAATCA TGCTGGAGAAACTTCGTTGTGCCATTCACTACCGTGAAGACCCCCTCAGTGGCTGATGGGAGGGAGCCCC AGAATTAGGCTGTCACTGAGGCACCCACTCTGCTGGCTTGGGAAGCCACCACTGCGGCCCGTCCCTCCAG GGCCCTGCGTGAGGACTTGGCAACATTTTGCTTTTCCAAACTTTCGTCCACATTCCAGGGCCTCCTGGAA GACTTAACCTTTTGTCTTTGTACGTTTCGTGATGGGTTGGTTCTTTTGCTGCCTGTTTGTGGTCTATTTG TAGGATAGTTTAGTTCCCAGACAGTTTGTGTCTAATTTGATCTTCTTGGACATTGTCCCTCATGGCCACC AGATTCTAATGCCATAAGGCCCCAGCTGGTTCCCCATCACAGAACCACCCAGCAGGAAGCCGGGGGTACA ATGACCTCGGCCACACAGAGCGCTCGTGCTTTCAGCAGAGCGCCTCCACAGACAAGCCTGTGGCCAGCCC TGCCCTCCAGCCTCGAGCTGGAGTTGCTCCAGAGGCAGCCCCAGCAGGCCTCCCTCCACATAGAGGTGGC TGGGCCAATTGCCCAAACACGTGAGACACACAGGGAGCTTTATGTGTTCATCTTCCTGCTTTAGAACTTG CTCCCGGTCTTGGTTTTTCTGAGGAAAAGCCCACAGTGGTTCCCCTGCCCCTCCCATACCTCCCTATCTG CCGTCTGTCTCTTTGGGCCCTGGTCTGACAGTTTTCATCTGAGGCCTCCTCGGGAGCAGGAGACTTAAGG GTTCATCCCGACCTTAGTTCACAGAGCCTAGCTGGGTGCATCCCAGCCGCAGGGGGTGAGAAGACACCCA GAGATGGGGGACAATGCTCAGGTGGGGGCCTCCTCCGAGACTCTGGTTGAGAGGAAAGCACATGATTCAA GCCTACCCCACAAAAAAGGAGCACAAAATGCTCAGAAATGGCCCTTCCTCCCTGCAGTGACGTAGGACAA GGGAAAGGGCCACACCCATTTTCAAAAGAGTGGACTGAGGTGCTCTGCTCAATTTGGGGTCTGCTTTAGT GACCCAGACACTCAAGTCCAGATGGAGACTCCAGGGGGGTCCTTCTGCCCCTCTGGCCAGCCCCACCCAG CACTCCCCATCACCCCATGTAAGTGTGTATGCCAGGGCTGAGCTGCCTCCAGGCTCACCCACTGCATCTT TGCCCCAAATAGGTCTTTGTCCAGTGGTACTTTGTTCATGAGCAGCACAGGCCATCTTGTTGGACACTGT CACTTTGGGCCTTGCCAGGTCTCACAGAATCACATCGCTGTATTTATTGTATAATATTGTGAATATTGGA AGTTTTGAGTGAGTGCTGTCTAGAAGGTTTTGGAGTGTGGATGCTGGTCCAAGAAAGAAGATGTGCCCTG GCCTGCTGGAAAGATTGTGAATTCTAGAGATAGAGCCGTTAGCATCCATCTCCTCCCTGTAACTCTTAGG AAATGCATCAAGGACCATTATAGAAAGGATCCCCTGACCTAAGGATGAAATCGACCTAGGGTTAATCATG ACAGCTGCATCAGTTGTATTGGATGCAAGTCAACTCTGGAGGTGAGTGCCCGCAAGCAGTTCCCCTGCTT TGAAAGAAGATGGTCAGCCCGCTGCCTCCCCAGCGGCCACAGCTCCTTTGAGTGTGGGGGGTCACAGCCA GGATGTATTCCTGACAATAAGGATCCTTTTATTTTCATTTCTGCATAATGAAAGAGCCTTTCGATTTATT AGAAGTGGTTTTTCCTCCCTACATCTTTAAATTCTTGGTTCTCTTTAGTCCTCATCCCTTGAATGGACTT GTCAGCTACCTCCCCAGGAGGAGTTCAGAAGCTTCTGTTGGCCATCACTTGAGTAGATGACAAGCTGTAA GGTATCTGCTGCTAACGTGGCTGCTTCTCCGAGAGCACATTGAGGTCCAGACAGATCCAAACCTTGGCCC TAAAAAATGTGTGCTGTAAAGTTACTTGATGTATATTTATTATAATTATTTATGGTTGCATTTAACAAAC TTTTCATTCAATCTGATGCTATTTACACCATGCTGTGTAAAGGAAGCTCACTAAAGGAAAAAAGTCACAC CAATAAATAACCTTCATCTAATTTTGATTTACCTTAAGAGTTGTGTGATCATCTACTCTTGCTGAGCAAG TTCAAAGGCTGGCATGCTTGGATGGCTCTGATATTTAAGTGCTGCCAAGTGGCTAGGAATTAAAGTGTTT CTAATTAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC006270 unedited NCCGGTCAGGATATTTGTATACGACTTACTATAGNNGGCGGCCGCGNAATTCGGCACCAG GGCAGACGTCCCGCTGCCCCTGGACCTCCTGCCCTCCTTCTGGAAGACGCTGGTGGGCGA GCCCTTGGACCCTGAGCAAGACCTGCAGGAAGCGGATATCCTCACCTACAATTACGTCAA GAAGTTTGAGAGCATCAATGATGAGACCGAGCTGGAGGCCCTGTGCGCTGAGATCGCCTC CCAGCACCTGGCCACGGAGAGCCCTGACAGCCCCAACAAGCCCTGCTGCAGGTTCACCTA CCTGACCATGACGGGCGAGGAGGTGGAGCTGTGCAGCCGGGGCCGGCACATCCTTGTGGC GTGGGAGAACAAGGACATCTACGCGGCAGCCATCCGGAGCCTGCGGCTGCGGGAGCTGCA GAATGTGGAGTGCGTGACGGCCGTGCGGGCCGGCCTGGGCTCCATCATCCCCCTGCAGCT GCTGACCATGCTCAGCCCACTGGAGATGGAGCTGCGCACCTGCGGCCTCCCCTACATCAA CCTCGAGTTCCTCAAGGCCCACACCATGTACCAAGTGGGGCTGATGGAGACGGACCAGCA CATCGAGTTCTTCTGGGGGGCCCTGGAGATGTTCACCCAGGAGGAGCTGTGCAAGTTCAT CAAGTTTGCCTGCAACCAGGAGCGCATNCCGTTCACCTGCCCCTGCAAAGATGGGGGTCC CGACACTGCCCATGTGCCCCCGTACCCCATGAAGATCGNCCCCCCAGATGGCACAGCAGG GTCCCCAGACTCTCGCTACATCCGCGTGGAGACCTGCATGTTCATGATCAAGCTTCCCCA GTACTCCTCTCTGGNAATCATGCTGGAGACTTTTCGGTGTGGCATTCACTACCGTGAAGA CCCCCTCAGTGGCTGATGGGAGGGAGGCCCAGAATAC
Restriction Sites	NotI-NotI
ACCN	BC006270
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006270.1, AAH06270.1
RefSeq Size	3045 bp
RefSeq ORF	351 bp
Locus ID	283450

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