TSFM (AF110399) Human Untagged Clone
CAT#: SC106436
(untagged)-Human elongation factor Ts mRNA, nuclear gene encoding mitochondrial protein, complete cds
CNY 2,400.00
CNY 6,270.00
货期*
现货
规格
Product images
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经常一起买 (4)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | EFTS; EFTSMT |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC106436 sequence for AF110399 edited (data generated by NextGen Sequencing)
ATGTCGCTGCTGCGGTCGCTGCGCGTGTTCCTGGTCGCGCGGACCGGGAGCTACCCGGCT GGGTCTCTTCTGCGTCAGTCGCCCCAGCCAAGGCACACATTTTATGCTGGGCCCCGTCTG TCTGCCTCGGCCTCCAGCAAGGAGCTCCTCATGAAGCTGCGGCGGAAAACAGGCTACTCC TTTGTAAATTGCAAGAAAGCTCTGGAGACTTGTGGCGGGGACCTCAAACAGGCAGAGATC TGGCTCCACAAGGAGGCCCAGAAGGAGGGCTGGAGCAAAGCTGCCAAGCTCCAAGGGAGG AAGACCAAAGAAGGCCTGATTGGGCTGTTGCAGGAAGGAAACACAACTGTATTAGTAGAG GTAAACTGTGAGACAGATTTTGTTTCTAGAAATTTAAAATTTCAACTGTTGGTCCAGCAA GTAGCCCTTGGAACCATGATGCATTGTCAGACCCTAAAGGATCAACCCTCTGCATACAGT AAAGGTTTCTTGAATTCCTCTGAGCTTTCTGGACTTCCAGCTGGGCCTGACAGAGAAGGC TCACTCAAGGATCAGTTGGCTTTAGCAATTGGAAAACTGGGAGAAAACATGATTCTTAAA CGAGCTGCATGGGTGAAGGTGCCATCTGGGTTCTACGTTGGCTCTTATGTCCACGGAGCA ATGCAGAGTCCCTCACTTCACAAGCTGGTGCTGGGGAAGTATGGGGCCCTGGTCATCTGT GAGACGTCTGAACAGAAAACAAACCTTGAAGACGTTGGCCGCCGCCTTGGGCAGCATGTG GTGGGCATGGCCCCCCTCTCTGTTGGCTCCCTGGACGATGAGCCTGGGGGAGAGGCAGAG ACTAAGATGCTGTCCCAGCCGTATTTGCTGGATCCCTCCATTACCTTGGGGCAGTATGTG CAGCCTCAGGGGGTGTCGGTAGTAGACTTTGTGCGGTTTGAATGTGGAGAAGGTGAAGAG GCAGCAGAAACTGAATAG Clone variation with respect to AF110399.1 30 t=>c >OriGene 5' read for AF110399 unedited
NNTTGTCAAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGGCCCGCC GGNAGGTGTTTATCGCGGCTAGAGAGATGTCGCTGCTGCGGTCGCTGCGCGTGTTCCTGG TCGCGCGGACCGGGAGCTACCCGGCTGGGTCTCTTCTGCGTCAGTCGCCCCAGCCAAGGC ACACATTTTATGCTGGGCCCCGTCTGTCTGCCTCGGCCTCCAGCAAGGAGCTCCTCATGA AGCTGCGGCGGAAAACAGGCTACTCCTTTGTAAATTGCAAGAAAGCTCTGGAGACTTGTG GCGGGGACCTCAAACAGGCAGAGATCTGGCTCCACAAGGAGGCCCAGAAGGAGGGCTGGA GCAAAGCTGCCAAGCTCCAAGGGAGGAAGACCAAAGAAGGCCTGATTGGGCTGTTGCAGG AAGGAAACACAACTGTATTAGTAGAGGTAAACTGTGAGACAGATTTTGTTTCTAGAAATT TAAAATTTCAACTGTTGGTCCAGCAAGTAGCCCTTGGAACCATGATGCATTGTCAGACCC TAAAGGATCAACCCTCTGCATACAGTAAAGGTTTCTTGAATTCCTCTGAGCTTTCTGGAC TTCCAGCTGGGCCTGACAGAGAAGGCTCACTCAAGGATCAGTTGGCTTTAGCAATTGGAA AACTGGGAGAAAACATGATTCTTAAACGAGCTGCATGGGTGAAGGTGCCATCTGGGTTCT ACGTTGGCTCTTATGTCCACGGAGCAATGCAGAGTCCCTCACTTCACAAGCTGGTGCTGG GGAAGTATGGGGCCCTGGTCATCTGTGAGACGTCTGAACAGAANACAACCTTGAAGACGT GGCCGCCGCCTTGGCAGCATGTGTGGGCATGGCCCCCTCTCTGTGGCTCCTGACAAGAGC CCTGGGGGAGAGCANAACTANNAGCTGTCCAGCCTAT >OriGene 3' read for AF110399 unedited
AGGTCCACTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTACGCAA ACAAGGAAAAAATATTTTTATTATACAACTATTTAATAATTTTACCCATGCATTCTCGGT CTGAAGAGGTTTGGGAAATATTCCTTTTTGTAATGATGTCCAGAGCTAAAAGTAAATATT CCTCCTGGGCCAAAAGTCTCTGGAACCTATTCAGTTTCTGCTGCCTCTTCACCTTCTCCA CATTCAAACCGCACAAAGTCTACTACCGACACCCCCTGAGGCTGCACATACTGCCCCAAG GTAATGGAGGGATCCAGCAAATACGGCTGGGACAGCATCTTAGTCTCTGCCTCTCCCCCA GGCTCATCGTCCAGGGAGCCAACAGAGAGGGGGGCCATGCCCACCACATGCTGCCCAAGG CGGCGGCCAACGTCTTCAAAGTTTGTTTTCTGTTCAGACGTCTCACAGATGACCAGGGCC CCATACTTCCCCAGCACCAGCTTGTGAAGTGAGGGACTCTGCATTGCTCCGTGGACATAA GAGCCAACGTAGAACCCAGATGGCACCTTCACCCATGCAGCTCGTTTAAGAATCATGTTT TCTCCCAGTTTTCCCATTGCTAAAGCCAACTGATCCTTGAGTGAGCCTTCTCTGTCAGGC CCAGCTGGGAGTCCAGAAAGCTCACAAGAATTTCAGGAAACTTTACTGTATGCAGAGGGG TGATCCCTTAGGGTCTGACAATGCATCATGGGTCCAAGGGCCTACTTGCTGGACCCAACA ATTGAAAATTTAAATTTNCAGAAACAAAATCTGGCCTACAGTTTACCCTTCTCTAACCAA TTGGGGTTTCCTTCCTGGAACAACCCCAAAAGGCCT |
Restriction Sites | NotI-NotI |
ACCN | AF110399 |
Insert Size | 1250 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | AF110399.1, AAD20224.1 |
RefSeq Size | 982 bp |
RefSeq ORF | 978 bp |
Locus ID | 10102 |
Domains | UBA, EF_TS |
Protein Families | Transcription Factors |
Gene Summary | This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010] |
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