Cullin 4B (CUL4B) (AF212995) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF212995, the custom clone sequence may differ by one or more nucleotides ATGGCTGAGGAATCCTCCTCCTCCTCCTCCTCATCTTCACCAACTGCTGCAACATCTCAGCAGCAGCAAC TTAAAAATAAGAGTATATTAATCTCTTCTGTGGCTTCGGTGCATCATGCAAACGGCCTAGCCAAATCTTC TACCACCGTCTCTAGCTTTGCTAACAGCAAACCTGGCTCTGCTAAGAACTTAGTGATCAAGAACTTTAAA GATAAGCCTAAATTACCAGAAAACTACACAGATGAAACCTGGCAAAAACTGAAAGAAGCAGTGGAAGCTA TTCAGAATAGTACTTCAATTAAGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATCTCTGTTCTTA CAAGATTTCTGCAAACTTGTACAAACAGCTGAGACAGATCTGCGAAGATCACATGAAAGCACAGATTCAT CAATTCAGAGAGGATTCATTGGATAGCGTTCTTTTTTTAAAGAAGATTGATAGATGCTGGCAAAACCATT GCAGACAAATGATCATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTACGTTCTTCAGAATTCAAT GCTACCCTCCATTTGGGACATGGGACTGGAGTTATTTAGGGCTCATATTATAAGTGATCAGAAAGTGCAG AATAAGACAATTGATGGCATTCTTCTCTTGATTGAGAGGGAAAGGAATGGTGAAGCAATTGATAGAAGTT TACTTCGAAGCCTTTTAAGCATGCTGTCTGATTTGCAAATTTATCAAGATTCTTTTGAACAACGATTTTT GGAAGAAACTAACCGGCTCTATGCAGCTGAAGGCCAAAAATTAATGCAAGAAAGAGAGGTTCCTGAATAT CTACATCATGTTAACAAACGTCTAGAAGAAGAAGCAGACAGACTTATTACTTACTTAGATCAGACCACCC AGAAGTCATTAATTGCTACTGTAGAAAAACAACTTCTAGGTGAACACTTAACAGCAATTCTTCAGAAAGG TTTAAATAACCTCCTTGATGAAAACCGAATTCAAGATTTGTCTCTTCTGTATCAGCTCTTCAGTAGAGTT CGAGGTGGAGTTCAGGTTCTTTTGCAGCAGTGGATCGAATATATCAAGGCATTTGGCAGCACTATTGTAA TTAATCCTGAAAAAGATAAAACCATGGTTCAAGAATTGCTGGATTTTAAAGATAAGGTTGACCATATAAT TGATATCTGCTTTCTGAAGAATGAGAAATTTATCAATGCCATGAAAGAAGCATTTGAAACGTTCATTAAC AAAAGACCAAATAAACCAGCTGAACTTATAGCTAAGTATGTAGATTCAAAACTTCGTGCAGGCAACAAAG AAGCTACAGATGAAGAACTTGAGAAAATGTTGGATAAAATTATGATCATATTTAGATTTATCTATGGCAA GGATGTTTTTGAGGCCTTCTATAAGAAAGATTTAGCCAAGCGCCTGTTAGTCGGAAAGAGTGCATCTGTA GATGCTGAAAAATCAATGCTGTCCAAACTTAAACATGAATGCGGAGCTGCTTTCACCAGCAAACTTGAAG GAATGTTTAAAGACATGGAACTTTCTAAAGACATCATGATTCAGTTCAAACAGGTAAAATATATGCAGAA TCAGAATGTTCCGGGAAATATTGAGTTAACTGTGAATATCCTGACAATGGGCTATTGGCCGACATATGTG CCTATGGAAGTTCATTTACCACCAGAGATGGTAAAACTTCAGGAGATTTTCAAGACATTTTACCTAGGCA AACATAGTGGCAGGAAACTTCAGTGGCAGTCAACCCTAGGACACTGTGTGTTAAAAGCAGAATTTAAAGA GGGTAAAAAGGAACTCCAGGTCTCTCTTTTTCAAACACTGGTGCTGCTAATGTTTAATGAGGGAGAGGAG TTCAGTTTAGAAGAGATCAAGCAGGCAACTGGAATAGAGGATGGAGAGTTAAGGAGAACACTGCAGTCAT TAGCCTGTGGCAAAGCTAGAGTTCTGGCGAAAAATCCAAAGGGCAAAGACATTGAAGATGGTGACAAGTT CATTTGTAATGATGATTTCAAACATAAACTTTTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACG GTTGAAGAACAAGCAAGCACTACAGAAAGAGTATTTCAAGACAGACAGTATCAAATTGATGCTGCAATTG TTCGAATTATGAAGATGAGAAAGACACTTAGCCACAATCTCCTTGTTTCAGAAGTGTACAACCAGTTGAA ATTTCCAGTAAAGCCTGCTGATCTTAAGAAGAGAATAGAATCTTTAATTGACCGGGACTACATGGAAAGA GATAAAGAAAATCCAAACCAGTACAACTATATTGCATAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF212995 unedited AGTATTTTGTAATACTTACTCACTATAGGGCGGCCGCGAATTCGCACGATAAGCCCGCCC CCTCCTCCTATCCACTTTTCTTCCCCCCATTTCTCCCTCCCCCCTTCCCCCCCCCACTCA AGTCCTGCGGACCTGGGGGCTTCGGGATCTGGGCGCTATGAAAAGTGTCTGCCCAGTCAC TTCCGGTTTTTCTTCCCCCAGTCCCTCAGCTGCTGCTGCTGCTCAGGAGGTCAGATCTGC CACTGATGGTAATACCAGCACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAACAG CAGCAGCAGTAGCAGCAGTAACAGTAGTAACGAGAGAGAAGACTTTGATTCCACCTCTTC CTCCTCTTCCACTCCTCCTTTACAACCCAGGGATTCGGCATCCCCTTCAACCTCGTCCTT CTGCCTGGGGGTTTCAGTGGCTGCTTCCAGCCACGTACCGATACAGAAGAAGCTGCGTTT TGAAGACACCCTGNAGTTTGTAGGGTTTGATGCGAAGATGGCTGAGGAATCCTCCTCCTC CTCCTCCTCATCTTCACCAACTGCTGCAACATCTCAGCAGCAGCAACTTAAAAATAAGAG TATATTAATCTCTTCTGTGGCTTCGGNGCATCATGCAAACGGCCTAGCCAAATCTTCTAC CACCGTCTCTAGCTTTGCTAACAGCAAACCTGGCTCTGCTAAGAAGTTAGTGATCAAGAA CTTTAAAAGATAGCCTAAATTACCAGAAAACTACACAGATGACACCCTGCAAAACTGAAN NGAGCAGTGGNAGCTATTCAGATAGTCCTTCAATTAGTACATNTAGAAAACTCTACCAGC TGNAGAAATCTTTGTCTAACAGATTCTGCAACTTGTCAAACAGCTGAGACGATCTGGGAG ATCCATTAAGCCCAATCCTCATTAGAGAGATTCTGGGTGCTTCTTTTTTTAAAGATGTAG TGCGCCAACCTTGGACAAGATAATAATTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF212995 unedited AAATACATCCAGNACTCTGTACAGTAAATAAAGTTTGCATGCAGTTTAAGTATCTTTAAA TGACATTTTAAGCAAATAAACCTTTTGCTTCATAATTAATGGTGTGTAAGAACCCTCCCC CCTTTTTAATAGCCACAGGGATCTCTTTCCCAGTTCCAAAGAGGAATTCATGAAGACCAG GTACGTTCTTAAGTGTTTTCTGCACATCATACTAAATAACATGCAAAGAGTTCAACAACA TTCTTCTTAAAGGTAAGTATTACTCTTTAAAATGGGCATGTTAACCACTGAGAAAAAGTC CACTCAACTATTTCCATTAATTACACTGCTTCATTTGGTCATCGGTAGTAAAAAAGGAGT CAAATAATATTGAATCAACAGGTTAGTTTATCCACTGGCTACTGCATATGACACCAAATG CTGCAAGGCCAACATTCTATGCAATATAGTTGTACTGGTTTGGATTTTCTTTATCTCTTT CCATGTAGTCCCGGTCAATTAAAGATTCTATTCTCTTCTTAAGATCAGCAGGCTTTACTG GAAATTTCAACTGGTTGTACACTTCTGAAACAAGGAGATTGTGGCTAAGTGTCTTTCTCA TCTTCATAATTCGAACAATTGCAGCATCAATTTGATACTGTCTGTCTTGAAATACTCTTT CTGTAGTGCTTGCTTGTTCTTCAACCGCTTCTTTCATCTGGATTTGATTGATCTTTATCC TGAAAAGTTATGTTTGAAATCATCATTACCAATGAACTTGTCACCATCTTTCATGTCTTT GCCCTTTGGATTTTTCGCCAGACCTCTAGCTTTGCCACAGGCTAATGACTGCAGTGTTCT CCTTTACCTCTCATCCCCTAT
Restriction Sites	NotI-NotI
ACCN	AF212995
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF212995.1, AAK16812.1
RefSeq Size	2810 bp
RefSeq ORF	2349 bp
Locus ID	8450
Domains	CULLIN
Protein Pathways	Nucleotide excision repair, Ubiquitin mediated proteolysis
Gene Summary	This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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