POLR3H (BC017248) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	5031409G22Rik; polymerase (RNA) III (DNA directed) polypeptide H; RPC8
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC017248, the custom clone sequence may differ by one or more nucleotides ATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTGAGAGGAAGCTCAACG ACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTACAACGTGGGACTCTGCATTTG TCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCATCACACACCAAAGTC TCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCCAGCCAAGTTCGACGAAG CGGAGCAGGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAGCACACGACCTCTACATGGACACCGGCGA GGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGTTGACACGTCCCCCACAGGGCCCAGCTCAGCAGAT GCCACCACTTCCAGTGAGGAGCTGCCAAAGAAGGAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGC CAGGCCTGGGCCTTCTCTCCTGGTGGACCAGCAACTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017248 unedited CACCAGGTGCGTGGTGCGTGGTGCGTGATACGAGCGGCGGCACGTGGCGCGGGTGCGGGG CGTGGAGTGGCGTGGCGTGGAGTGGCGTGGCGTGGCGGGGTCTCGCGGCGCGGGCGCGCA CCCGGAGCTGTGGACGGAGAGTGCCTCCCTCTGGCCTCAGTTTCCTCATGTTGTAGTAGC GGAGCATGGCCCGGACCGGGCCCCCGAGACCGCCCCGTGCAACCTCACCGCCAGCCTGGG GGCCTCAGCGACTGGGACGGGACCAAGGGGCTCGGGGATTCTCCCTGCCCCCGGCCCTGG TGCGTGACTGACCCTCCTGTTCCCAGAGCCCCCAGCGCAGGCCGGGATGTTCGTCCTGGT GGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTGAGAGGAAGCTCAACGACTC CATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTACAACGTGGGACTCTG CATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGC ATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATCCATTCCTAGATGAGATTCT CATTGNGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACGTCTCTCTAGGCTTCTTCGA TGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC017248 unedited TTGAAACGTATAGCTAAGNACGCGCCGCAATCAGGATCGATTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCAGGCAA CGGTTTTTATTTTGGAAGACAAAACCCCCATCTAAAGTTTTTCCCCGGCCGGATCATTTC ATGGGGGGCGGAAGGATTCTAAAAAACCTTTTGTTCTGGCAAGGAAAAAAAAAATCCAAA ACCAGGGACTGTTCTGGAAAAAATGGGGGTCTGGCCCCTTTGTTGTAAAACCCAGGGCCC AAAAAGGGTCTTTTCAACCAAGGGCAGGGGAGGCCCAACACCCCCAGCCCCAAATGGACC CCCCCCTGCAGGAAAAGAGGAACAGGGCCTAAATGGGGGAGAAACGGGGCAAGCTGGGGG GCACCCCGGGCCTTGCCTAATTGGCCAGCTGGAAACACTTTCTCCTAAAAAACGGCCCAA GCTGGTGTGGTTTTAAAAGCCGGCCAAACCACCTTCCCGAAGGGGGGGAGGGCCCACTGC CCACCCCCAGGGTTAGTTGCGGGCCCCCCAAAAAAAAAAGCCCAGCCCGGGCTCACGGGG GGACCCCAAAAGCGGGGACGGACCCCCCTTTTTTGGGACCCCCCCCCGGGAAAGTGGGGG CATCTGCTAAACTGGGCCCTGGGGGGGACGGTGAAAAAAAGCTCTGGCCCACCACCCGGA ACCGGATCTCCCCGCCGGGGCCCTTGTAAAGGGCGGGGGCCCCTTTCCCCGGTTGGTAAT CCCAACCCAAACCTGCTCCCGTTTGAAAAATGGGCTGGCGGGCAAAG
Restriction Sites	NotI-NotI
ACCN	BC017248
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017248.2, AAH17248.1
RefSeq Size	1276 bp
RefSeq ORF	528 bp
Locus ID	171568
Domains	RNA_pol_Rpb7_N
Protein Families	Transcription Factors
Protein Pathways	Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
Gene Summary	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function]

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