FAM125A (MVB12A) (BC011840) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CFBP; CIN85/CD2AP family binding protein; family with sequence similarity 125, member A; FLJ32495
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC106275 sequence for BC011840 edited (data generated by NextGen Sequencing) ATGGATCCCGTACCCGGGACAGACTCGGCGCCGCTGGCTGGCCTGGCCTGGTCGTCGGCC TCTGCACCCCCGCCGCGGGGGTTCAGCGCGATCTCCTGCACCGTCGAGGGGGCACCCGCC AGCTTTGGCAAGAGCTTCGCGCAGAAATCTGGCTACTTCCTGTGCCTTAGTTCTCTGGGC AGCCTAGAGAACCCGCAGGAGAACGTGGTGGCCGATATCCAGATCGTGGTGGACAAGAGC CCCCTGCCGCTGGGCTTCTCCCCCGTCTGCGACCCCATGGATTCCAAGGCCTCTGTGTCC AAGAAGAAACGCATGTGTGTGAAGCTGTTGCCCCTGGGAGCCACGGACACGGCTGTGTTT GATGTCCGGCTGAGTGGGAAGACCAAGACAGTGCCTGGATACCTTCGAATAGGGGACATG GGCGGCTTTGCCATCTGGTGCAAGAAGGCCAAGGCCCCGAGGCCAGTGCCCAAGCCCCGA GGTCTCAGCCGGGACATGCAGGGCCTCTCTCTGGATGCAGCCAGCCAGCCAAGTAAGGGC GGCCTCCTGGAGCGGACAGCGTCAAGGCTGGGCTCTCGGGCATCCACTCTGCGGAGGAAT GACTCCATCTACGAGGCCTCCAGCCTCTATGGCATCTCAGCCATGGATGGGGTTCCCTTC ACACTCCACCCACGATTTGAGGGCAAGAGCTGCAGCCCCCTGGCCTTCTCTGCTTTTGGG GACCTGACCATCAAGTCTCTGGCGGACATTGAGGAGGAGTATAACTACGGCTTCGTGGTG GAGAAGACCGCGGCTGCCCGCCTGCCCCCCAGCGTCTCATAG Clone variation with respect to BC011840.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011840 unedited TAGATTTTGTAATACGACTTACGTATAGGGCGGCCGCGAATTCGCACCAGGTGCAGACTT GCAGAGAACGAGTCTCTTGGTCCTGTGCCGTAGCATTTCCGGCGGAAGTTGCTGGGCGCG GAAAGCCGGCAAGACCTTTTTTTCCGCCATCATCGCTCACGCGCGCAACCCTGGCGACGC GTGGTGGCGAGGCTCCGCCTCCCCACGCCATTGGCCAGCAGCTCCTCGGGCACGCGTATA CTGCGCTTCCGGGCAATCTCCGCTCCCCCCCACCCCCCCGCATGGCCTTCTGGGAGTTGT AGTTCGGTCGCGAGCGCTGCCGTCGGGAGGCGCTCCGAGGTTCGAGGCTGTGCCCCGCGA CCCCGCCTTCGGCGCTCGGCTCGCAGGATGGATCCCGTACCCGGGACAGACTCGGCGCCG CTGGCTGGCCTGGCCTGGTCGTCGGCCTCTGCACCCCCGCCGCGGGGGTTCAGCGCGATC TCCTGCACCGTCGAGGGGGCACCCGCCAGCTTTGGCAAGAGCTTCGCGCAGAAATCTGGC TACTTCCTGTGCCTTAGTTCTCTGGGCAGCCTAGAGAACCCGCAGGAGAACGTGGTGGCC GATATCCAGATCGTGGTGGACAAGAGCCCCCTGCCGCTGGGCTTCTCCCCCGTCTGCGAC CCCATGGATTCCAAGGCCTCTGTGTCCAAGAAGAAACGCATGTGTGTGAAGCTGTTGCCC CTGGGAGCCACGGACACGGCTGTGTTTGATGTCCGGCTGAGTGGGAAGACCAAGACAGTG CCTGGATACCTTCGAATAGGGGACATGGGCGGCTTTTGCATCTGGTGCAAGAAGGGCCAG GCCCCGAGCCAGTGCCCAGCCCCCGAGTCTCAGCCGGGACATGCAGGCCTTCTCTGATGC AGCCGCCAGCCAGTTAGGCGGNCTCCTGNACGGACACGTCAGGCTGGCTTTGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC011840 unedited NGGGCCCTCTATGTACGCGGCCGCTTTTAGNAGTCGGTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTGCGAAAACCGGGTAGTGTTTATTGCCAGGAGGACACCCGCACAAC CTCCGGACCCCAAAGGGTTTTGACACAAATGACTTAAAGGCACTGGCTTTTGTCCAGCTC CAGCCCTGCCCGGCAGGGCGTGGGGGCGGAAACCCCATTACCACGCAACCTCGACCCGCC CCCTCCTCCAAGGCTGCAGCTCCGCCCAGTGGCTGAAAATAGCAAACGCCTTGCAAGGCG AAAGTTCCCAAGATGCAGTGAGGGGGGGTGGCCCCAAGCTGGCGGGGAAGTGGAATAAAG GGGCTCTTTCCGCGGAAAGGTGAAGGACTATGAAACCCTGGGGGGCAAGCGGGCAACCCC GGTCTTCTCCACCACGAAACCGTAGTTATACTCCTCCTCAATGTCCGCCAGAAACTTGAT GGTCAAGTCCCCAAAAACAAAAAAAGCCAAGGGGCTGCAGCTCTTGCCCTCAAATCGTGG GTGGAGTGTGAAAGGAACCCCATCCATGGCTGAGATGCCCTAAAAGCTGGAAGCCTCGTA AATGGAGTCATTCCTCCGCAGAGTGGATGCCCGAGAGCCCAGCCTTGACGCTGTCCGCTC CAAGAAGCCGCCCTTACTTGGCTGGCTGGCTGCATCCAGAGAGAAGCCCTGCATGTCCCG GCTGAAACCTCGGGGCTTGGGCACTGGCCTCGGGGCCTTGGGCTTCTTGCACCAGATGGC AAAACCGCCCATGTCCCCTATCCGAAGTATCCAAGCACTGTCTTGGGTCTTCCCANTCAA GCGGGAATCAAACACAGCCGGTGTCC
Restriction Sites	NotI-NotI
ACCN	BC011840
Insert Size	1600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011840.2, AAH11840.1
RefSeq Size	1216 bp
RefSeq ORF	822 bp
Locus ID	93343
Protein Pathways	Endocytosis
Gene Summary	Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in the ligand-mediated internalization and down-regulation of EGF receptor.[UniProtKB/Swiss-Prot Function]

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