Calpastatin (CAST) (BC016066) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BS-17; calpain inhibitor; calpastatin; heart-type calpastatin; MGC9402; OTTHUMP00000158519; OTTHUMP00000158520; sperm BS-17 component
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC016066, the custom clone sequence may differ by one or more nucleotides GGAGCCCGGGTCCCTCCGCGGGCAGGAAGGGGAGGGCCCATCGGGGCTAGGGGAACCCCGGCGCAGACCT GGGGTGGGGTCGGAAAAGCTGTTTCATCGCCCGCTCCCGGGCCCTCCGCTCCCTCCCTCCCTCCCTCTCT CCCTGGCAGGACTCCCCGCCAGGCCTCCCCGCCACTCTCCGCGGCGCATTCCGGGAGGCAGCGGCCGCAG CGGCCTCGCCATGTCCCAGCCCGGCCAGAAGCCCGCCGCCTCCCCGCGGCCCCGGCGAGCAGCCGCCGCC CGCCGCACCCATGAGCATGTCAGTGAAAAAACCAGTGAATCGCCTTCCAAACCAGGAGAAAAGAAAGGAT CAGATGAGAAAAAAGCAGCAAGCCTCGGCAGCAGTCAATCCTCCAGAACCTATGCTGGTGGAACAGCCTC GGCCACCAAGGTGTCAGCTTCCTCTGGTGCAACCAGCAAGTCTTCCAGTATGAATCCCACAGAAACCAAG GCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCTGTGGTTCATGAGAAAAAATCCC AAGAAGGAAAGCCAAAAGAACACACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAGTAA CGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCCATCAGAGAAATCAACAGAACCA AAGACTAAACCACAAGACATGATTTCTGCTGGTGGAGAGAGTGTTGCTGGTATCACTGCAATATCTGGCA AGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTAACCCCAGCTGTGCCAGTTGAATCTAAACCGGATAA ACCATCGGGAAAGTCAGGCATGGATGCTGCTTTGGATGACTTAATAGATACTTTAGGAGGACCTGAAGAA ACTGAAGAAGAAAATACAACGTATACTGGACCAGAAGTTTCAGATCCAATGAGTTCCACCTACATAGAGG AATTGGGTAAAAGAGAAGTCACAATTCCTCCAAAATATAGGGAACTATTGGCTAAACCCATAGGGCCAGA TGATGCTATAGACGCCTTGTCATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACTGAA AAAGAGGAATCTACAGAAGTTTTAAAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCACCCCAAG AGAAGAAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCACTGGG CACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAAAAAAAAAA AAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC016066 unedited AAGGTTCACATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCATCG GGGCTAGGGGAACCCCGGCGCAGACCTGGGGTGGGGTCGGAAAAGCTGTTTCATCGCCCG CTCCCGGGCCCTCCGCTCCCTCCCTCCCTCCCTCTCTCCCTGGCAGGACTCCCCGCCAGG CCTCCCCGCCACTCTCCGCGGCGCATTCCGGGAGGCAGCGGCCGCAGCGGCCTCGCCATG TCCCAGCCCGGCCAGAAGCCCGCCGCCTCCCCGCGGCCCCGGCGAGCAGCCGCCGCCCGC CGCACCCATGAGCATGTCAGTGAAAAAACCAGTGAATCGCCTTCCAAACCAGGAGAAAAG AAAGGATCAGATGAGAAAAAAGCAGCAAGCCTCGGCAGCAGTCAATCCTCCAGAACCTAT GCTGGTGGAACAGCCTCGGCCACCAAGGTGTCAGCTTCCTCTGGTGCAACCAGCAAGTCT TCCAGTATGAATCCCACAGAAACCAAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAG TCAACCAAGCTGTCTGTGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCAAAAGAACAC ACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAAT AAANAAGCAGTTTCCAGATCAGCTGAACAGCAGCCATCAGAGAAATCAACAGAACCAAAG ACTAAACCCACAGACATGATTTCTGCTGGTGGAGAGAGTGTTGCTGGTATCACTGCAATA TCTGGCAAGCCGGNNTGACAGAAAAAGAAAGAAATCATAACCCCAGCTGTGCCAGTTGAA TCTAAACCGATAAACCATCGGNAAAGTCAGCATGGATGCTGCTTNGNATGACTTAATAGA AC
Restriction Sites	NotI-NotI
ACCN	BC016066
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016066.1, AAH16066.1
RefSeq Size	1335 bp
RefSeq ORF	1335 bp
Locus ID	831
Domains	Calpain_inhib
Gene Summary	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

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