TAT (BC020707) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	tyrosine aminotransferase; tyrosine aminotransferase, cytosolic
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC020707, the custom clone sequence may differ by one or more nucleotides ATGGACCCATACATGATTCAGATGAGCAGCAAAGGCAACCTCCCCTCAATTCTGGACGTGCATGTCAACG TTGGTGGGAGAAGCTCTGTGCCGGGAAAAATGAAAGGCAGAAAGGCCAGGTGGTCTGTGAGGCCCTCAGA CATGGCCAAGAAAACTTTCAACCCCATCCGAGCCATTGTGGACAACATGAAGGTGAAACCAAATCCAAAC AAAACCATGATTTCCCTGTCCATTGGTGAGTTGGGGACACTACTGAGGGGCTGTCATTGTCCTCCTCTAC TCTCATGTTCACAGGCTGGTTGGAGAAGATGGCAGTTGGGGGTGAGTCTGAGTACTGAACATGGGAGAAT AACCTCTTGGCTCCTTTTGTGTTTTCCTCCCATAAAAAGGGGACCCTACTGTGTTTGGAAACCTGCCTAC AGACCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020707 unedited GCGGTCACATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGCAAANAAA AGCTAAGGGAGCTTTCGGGGTTGGCTTCTTGGAGGCTGCTTTCTCCTTTACTTGGAAGGC TTCGCTAGTGATGGACCCATACATGATTCAGATGAGCAGCAAAGGCAACCTCCCCTCAAT TCTGGACGTGCATGTCAACGTTGGTGGGAGAAGCTCTGTGCCGGGAAAAATGAAAGGCAG AAAGGCCAGGTGGTCTGTGAGGCCCTCAGACATGGCCAAGAAAACTTTCAACCCCATCCG AGCCATTGTGGACAACATGAAGGTGAAACCAAATCCAAACAAAACCATGATTTCCCTGTC CATTGGGGACCCTACTGTGTTTGGAAACCTGCCTACAGACCCTGAAGTTACCCAGGCAAT GAAAGATGCCCTGGACTCGGGCAAATATAATGGCTATGCCCCATCCATCGGCTTCCTATC CAGTCGGGAGGAGATTGCTTCTTATTACCACTGTCCTGAGGCACCCCTAGAAGCTAAGGA CGTCATTCTGACAAGTGGCTGCAGCCAAGCTATTGACCTTTGTTTAGCTGTGTTGGCCAA CCCAGGGCAAAACATCCTGGTTCCAAGACCTGGTTTCTCTCTCTACAAGACTCTGGCTGA GTCTATGGGAATTGAGGTCAAACTCTACAATTTGTTGCCAGAGAAATCTTGNGAAATTGA CCTGAACAACTGGAATATCTAATTGATGAAAAGACAGCTTGTCTCATTGTCAATAATCCA TCAAACCCCTGTGGGTCAGTGTTCAGCAAACGTCATCTTCAGAAGATTCTGCAGTGGCTG CACGGCAGTGTGTCCCCATCTTAGCTGATGAGATCTATGGAGACATGGTGTTTTTCNGAT TTGCAATATGAAACACTGGCCAC
Restriction Sites	NotI-NotI
ACCN	BC020707
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020707.1, AAH20707.1
RefSeq Size	1116 bp
RefSeq ORF	1116 bp
Locus ID	6898
Protein Families	Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
Protein Pathways	Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism, Ubiquinone and other terpenoid-quinone biosynthesis
Gene Summary	This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible cognitive disability. A regulator gene for tyrosine aminotransferase is X-linked. [provided by RefSeq, Jul 2008]

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