C12orf48 (PARPBP) (AF274940) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AROM; C12orf48; PARI
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF274940, the custom clone sequence may differ by one or more nucleotides ATGGCGAGACCTCGTCTCTACAAAAAAATACAAAAATTAGCTGGGTGTGGTGGGAGTGTGCCTGTAGTCT CAGCTACTTGGGAGACTGAGCACAGTGGAGAATTTACAGTCTCTCTCAGTGATGTTTTATTGACATGGAA ATACTTGCTCATGAGAAATTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF274940 unedited TTCCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG AATTCGGCACGAGGCTGCGGCGGCCGCGGGAGGGCATCCCGTTGGGGATCCTTCCGCACA CTGAAGAGTACGTCTTCGGGTCTACCCCTAATCACATAATGGCTGTGTTTAATCAGAAGT CTGTCTCGGATATGATTAAAGAGTTTCGAAAAAATTGGCGTGCTCTTTGTAACTCTGAGA GAACTACTCTATGTGGTGCAGACTCCATGCTCTTGGCATTGCAGCTTTCTATGGCGGAGA ACAACAAACAGAGTCAACTACTGGATTTTCTGTCTGGCAAACAGTATGCAGTAGGTGATG AAACTGATCTTTCTATACCAACATCACCAACAAGTAAATACAACCGTGATAATGAAAAGG TGCAGCTGCTAGCAAGGAAAATTATCTTTTCATATTTAAATCTGCTAGTGAATTCAAAGA ATGACCTGGCTGTGGCTTATATTCTCAATATTCCTGATAGAGGACTAGGAAGAGAAGCCT TCACTGATTTGAAACATGCTGCTCGAGAGAAACAAATGTCTATCTTTTTGGTGGCCACGT CTTTTATTAGAACAATAGAGCTTGGAGGGAAAGGATATGCACCACCACCATCAGATCCTT TAAGGACACATGTAAAGGGATTGTCTAATTTTATTAATTTCATTGACCAATTAGATGAGA TTCTGGNAGAAATACCAAACCCAAGCATTGCAGGNNNGTCAAATACTGTCAGTGATNAAG ATGCCACTGATTAAGGCCANAACAGCAGGNATCCTTTTTGCAAGCATAGAGGAAGTTGCT CAGATTGGATTTGAGGATAAAAATATATCATTCTCAGAAGGTGTGTAGCTCTTACACN
Restriction Sites	NotI-NotI
ACCN	AF274940
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF274940.1, AAK07517.1
RefSeq Size	1223 bp
RefSeq ORF	162 bp
Locus ID	55010
Protein Families	Druggable Genome
Gene Summary	Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Binds single-strand DNA and poly(A) homopolymers. Positively regulate the poly(ADP-ribosyl)ation activity of PARP1; however such function may be indirect.[UniProtKB/Swiss-Prot Function]

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