AP3S2 (AL110108) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AP3S3; sigma3b
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL110108, the custom clone sequence may differ by one or more nucleotides CTGAGAGAACCGTGGCTGGCAAAGATGATTCAGGCGATTCTGGTTTTCAACAACCATGGGAAGCCACGGC TAGTCCGCTTCTACCAGCGTTTCCCANAAGAAATTCAACAGCAGATTGTTCGAGAGACTTTCCATCTANT CCTCAAGCGGGATGACAACATCTGTAACTTCTTGGAGGGTGGAAGTTTGATTGGTGGCTCTGACTACAAA CTGATCTACCGGCACTATGCTACCCTCTACTTTGTATTTTGTGTGGATTCCTCAGAGAGTGAACTTGGAA TCTTGGACCTCATCCAGGTTTTTGTGGAAACTCTGGATAAGTGTTTCGAAAATGTGTGTGAATTGGATTT GATCTTCCATATGGATAAGGTGCACTACATCCTCCAGGAAGTGGTGATGGGTGGGATGGTGTTGGAAACA NACATGAATGAAATCGTGGCTCANATTGAGGCTCAAAACANGCTGGGAGAAATCCCGAAGGGTGGCCTTT CAGCAGCCCCTGGCGCGGGCTGTGTCCTGCTGTGAAAAAAACATCAAACCTGGCCAAGAGAATTCCTCCG GAACATCAACAATTGGGCGATTTTCAACATCAAAAGTTCCCAAACCT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL110108 unedited TACCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA TTCGGCACGAGGCTTTCTGGGAGTAGCCGGTGCTGAGAGAACCGTGGCTGGCAAAGATGA TTCAGGCGATTCTGGTTTTCAACAACCATGGGAAGCCACGGCTAGTCCGCTTCTACCAGC GTTTCCCAGAAGAAATTCAACAGCAGATTGTTCGAGAGACTTTCCATCTAGTCCTCAAGC GGGATGACAACATCTGTAACTTCTTGGAGGGTGGAAGTTTGATTGGTGGCTCTGACTACA AACTGATCTACCGGCACTATGCTACCCTCTACTTTGTATTTTGTGTGGATTCCTCAGAGA GTGAACTTGGAATCTTGGACCTCATCCAGGTTTTTGTGGAAACTCTGGATAAGTGTTTCG AAAATGTGTGTGAATTGGATTTGATCTTCCATATGGATAAGGTGCACTACATCCTCCAGG AGGTGGTGATGGGTGGGATGGTGTTGGAAACAACATGAATGAATCGTGGCTCAGATTGAN GCTCANAACANGCTGNAGAAAATCGAGGNNTGCCTTTCAGCAGNCCCTGCGCGGGCTGTG TCTGCTGTGNAAAACATCACCTGCCAGAGATTCCCTCGAAACATCACATTNGCGATCTCA ACATCAAATTCCCCAACTGTCCCCAGTTGTCTGAGATCAAGTATGNCCTGAATAGAGTCC TTAGACCAGCAAGACAAGCAGGCAGCCCGTCTGGAAACGACCNATTTGGGAGCTAGAGAA TCAAGCCTCAGACTGNAAACTTGGTCTGGAAACTGTTTGCATGAATAGAAGGATCCTTTG ACCTGCTGGTGCC
Restriction Sites	NotI-NotI
ACCN	AL110108
Insert Size	2800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL110108.1
RefSeq Size	607 bp
RefSeq ORF	607 bp
Locus ID	10239
Domains	Clat_adaptor_s
Protein Pathways	Lysosome
Gene Summary	Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.[UniProtKB/Swiss-Prot Function]

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