ZNF93 (BC020837) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HPF34; HTF34; TF34; ZNF505
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC020837, the custom clone sequence may differ by one or more nucleotides GTCTCTCGGTGCAGCCGGAGCTCCAGGTCTCCTCTTCACTACTCTGTGTCCTGTGCTCCTACAGGCCCAG CCTCTGTGGCCCTGTGACCTGCAGGTATTGGGAGATCCACAGCTAAGACACCAGGACCCCTGGAAGCCTA GAAATGGGACCATTGCAATTTAGAGATGTGGCCATAGAATTCTCTCTGGAGGAGTGGCATTGCCTGGACA CTGCACAGCGGAATCTATATAGGAATGTGATGTTAGAGAACTACAGTAACCTGGTCTTCCTTGGTATTGT TGTCTCTAAGCCAGACCTGATCGCCCATCTGGAGCAAGGAAAAAAACCTTTGACTATGAAGAGACATGAG ATGGTAGCCAACCCCTCAGGTGCACGCCTGTTATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATCCCG AGGTCAGGAGATCGAGACCATCTGGCCAACATGGTGAAATCCCATCTCTACTAAGATACAAAAAATTAGC CAGGCATGGTGGCCCATGCCTGTAGTACGAGCTACTCGGGAGGTTGAGGCAGGGGAATCACTAGAACCCA GGAGGCACTCCAGCCTGGGAGACAGAGTGAGACTCCATCTCAAAATATATATATATTTCCAAATATTCAG TTTGGTCTTATTATATTGACATTGTTATGCAACATATCACTAGAGTGTTTTTTATCTTGCAAAGCTAAAT CTCAGTACACAGTAAATAACTACCAATCCTTTGTATTTTGTGGTTCTTTTCAAACACCACTCTGTTTTCT GTTTCTAAGAGTATAACTGCTTTATATATCTCATCCAATCTCTGTCTTTTTGCGGCTGGGTCACTTTATT TTGCATAATGTCATCAATATTTATCTTAATAGTTGTTACAGTATTTTCTGCTTTTTGAAAACTGAGTGAT AATTCCTGTACTTTTGTGTCTACTGAATGATTTGGTGACACAAATTTCGATTACTTTTACCTAGTGGCTT GCAGTAACAATGCTACAATAATTATGTGAAAGTTAATGTAATATATTTGCTGCATTCTGTTTTATTAGTC TACTTTTTCACCTTTATACTCATACCAAATTATTTTAATTCTGTAGCTTTTTGATGTGTTTTGAAATCAG AAATGGTATTGCCTGTAACATTGTTCCTGTTTTTGAAGATTGCTGGGTACTTTATTGTCTGTTTGGATTC CATATACTTTTAAATTTGTTGTTTTTATTTCTTTGAAAATGCTGTGAGTCATTTGAAAGACATTGCATTA AATCTGTAGATTAAATTGAGCAGTAAAGACATCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020837 unedited NTTTACACCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC GAATTCGGCACGAGGGTCTCCTCTTCACTACTCTGTGTCCTGTGCCCCTACAGGCCCAGC CTCTGTGGCCCTGTGACCTGCAGGTATTGGGAGATCCACAGCTAAGACACCAGGACCCCT GGAAGCCTAGAAATGGGACCATTGCAATTTAGAGATGTGGCCATAGAATTCTCTCTGGAG GAGTGGCATTGCCTGGACACTGCACAGCGGAATCTATATAGGAATGTGATGTTAGAGAAC TACAGTAACCTGGTCTTCCTTGGTATTGNTGTCTCTAAGCCAGACCTGATCGCCCATCTG GAGCAAGGAAAAAAACCTTTGACTATGAAGAGACATGAGATGGTAGCCAACCCCTCAGTT ATATGTTCTCATTTTGCCCAAGATCTTTGGGCCGAGCAGAACATACAAGATTCTTTTCCA AAAGTGATACTGAGAACAAAGAAAAACGTGGACATGGAAATTTACCGTCAATACAAAGGC GTGCAAGTGTTCTACATCTCCACTGTCCCCCCCCTCCGCCTCTACGCCCAATCCCCTCCT CCCCCTCATCTTCCCCCCCCCCCCACCACCCTCCTCCCCACACCCACCCTCTACCCACTC CCTCTCTTTCCCCTTCTTCTACACTCCACCCCTATTAACTCTCACCTCCACCACCTCTCC CCCCCCCCCCCCCTTCCCCCCTCCCATACTTTTCCCTTCATCTCTCCTTCCCACCACTCC CCATCTCTCTCCCCCCCTCTCTCTCCGCCACGTCTATCTCACTTCCTCTCCCCTTCCTCT TTCTATCTCGTCACTTTTCTTTTCTTTTTTATCATCTCTCCTTTTT
Restriction Sites	NotI-NotI
ACCN	BC020837
Insert Size	3300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC020837.1
RefSeq Size	1327 bp
RefSeq ORF	1327 bp
Locus ID	81931
Domains	KRAB
Protein Families	Transcription Factors
Gene Summary	Transcription factor specifically required to repress long interspersed nuclear element 1 (L1) retrotransposons: recognizes and binds L1 sequences and repress their expression by recruiting a repressive complex containing TRIM28/KAP1 (PubMed:25274305). Not able to repress expression of all subtypes of L1 elements. Binds to the 5' end of L1PA4, L1PA5 and L1PA6 subtypes, and some L1PA3 subtypes. Does not bind to L1PA7 or older subtypes nor at the most recently evolved L1PA2 and L1Hs. 50% of L1PA3 elements have lost the ZNF93-binding site, explaining why ZNF93 is not able to repress their expression (PubMed:25274305).[UniProtKB/Swiss-Prot Function]

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