GNB5 (BC011671) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ37457; FLJ43714; GB5; G protein, beta-5 subunit; G protein, beta subunit 5L; guanine nucleotide-binding protein, beta-5 subunit; guanine nucleotide-binding protein, beta subunit 5L; guanine nucleotide binding protein (G protein; transducin beta chain 5
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC011671, the custom clone sequence may differ by one or more nucleotides ATGGCAACCGAGGGGCTGCACGAGAACGAGACGCTGGCGTCGCTGAAGAGCGAGGCCGAGAGCCTCAAGG GCAAGCTGGAGGAGGAGCGAGCCAAGCTGCACGATGTGGAGCTGCACCAGGTGGCGGAGCGGGTGGAGGC CCTGGGGCAGTTTGTCATGAAGACCAGAAGGACCCTCAAAGGCCACGGGAACAAAGTCCTGTGCATGGAC TGGTGCAAAGATAAGAGGAGGATCGTGAGCTCGTCACAGGATGGGAAGGTGATCGTGTGGGATTCCTTCA CCACAAACAAGGTGAGGCATTGTTCCCAGCCACGTTATAGAGGCTTCAGAATCCCAGCCTACTTAAAGGT GCTCTGGTCCAGCTGCCCGGCCCTGTCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011671 unedited GCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCAGCGCAT TCCCGCTCTCCGCTTCCCTCTCCGCTGCGTCCCCGCGCGAAGATGGCAACCGAGGGGCTG CACGAGAACGAGACGCTGGCGTCGCTGAAGAGCGAGGCCGAGAGCCTCAAGGGCAAGCTG GAGGAGGAGCGAGCCAAGCTGCACGATGTGGAGCTGCACCAGGTGGCGGAGCGGGTGGAG GCCCTGGGGCAGTTTGTCATGAAGACCAGAAGGACCCTCAAAGGCCACGGGAACAAAGTC CTGTGCATGGACTGGTGCAAAGATAAGAGGAGGATCGTGAGCTCGTCACAGGATGGGAAG GTGATCGTGTGGGATTCCTTCACCACAAACAAGGAGCACGCGGTCACCATGCCCTGCACG TGGGTGATGGCATGTGCTTATGCCCCATCGGGATGTGCCATTGCTTGTGGTGGTTTGGAT AATAAGTGTTCTGTGTACCCCTTGACGTTTGACAAAAATGAAAACATGGCTGCCAAAAAG AAGTCTGTTGCTATGCACACCAACTACCTGTCGGCCTGCAGCTTCACCAACTCTGACATG CAGATCCTGACAGCGAGCGGCGATGGCACATGTGCCCTGTGGGACGTGGAGAGCGGGCAG CTGCTGCAGAGCTTCCACGGACATGGGGCTGACGTCCTCTGCTTGGACCTGGCCCCCTCA GAAACTGGAAACACCTTCGTGTCTGGGGGATGTGACAAGAAGCCATGGTGTGGGACATGC GCTCGGCCAGTGCGTGCAGGCCTTTGAACACATGAATCTGACTCAACAGTGTCCCGTACT ACCCCAGTGGAGATGCCTTTGCTTAGGNTCAGAGACGCTACGTGTCGCCTCTATGACTGC GGCAGAAAGGNAGGTGCCATCTATTCAAGAAAGCATCTATTTGAGCTNCAGCGTGACCTN CCTCATGGCGNCTGCTGNTGCTGATCATGATAACTAA
Restriction Sites	NotI-NotI
ACCN	BC011671
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011671.1, AAH11671.1
RefSeq Size	943 bp
RefSeq ORF	381 bp
Locus ID	10681
Domains	WD40
Protein Families	Druggable Genome
Protein Pathways	Chemokine signaling pathway
Gene Summary	Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008]

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