Integrin Linked Kinase (ILK) (AL833496) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TAF2A; TAF2H; TAFII30
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL833496, the custom clone sequence may differ by one or more nucleotides GGGGACGGGTGAGGGCGAGGGAAGCGTCTTGGGCTGGGTCCAGCGGCGGGTGAGGGCAGAGGGTATAGAC AGACCTAGCTGGGGCCCTGCGCCTCCCACCTCTGTGTCTCATTTTGCTGGTTCTCCTCCAGCTCCGGTGT CGGCGGGTGGCGCGGCGCCCCCGGAGGGGGCCATATCTAACGGGGTTTACGTACTGCCGAGCGCGGCCAA CGGAGACGTGAAGCCCGTGGTGTCCAGCACGCCTTTGGTGGACTTCTTGATGCAGCTGGAAGATTACACG CCTACGGTGGGCTTCCGCCCGAACAAGGCCACCTAGCCTGCTGACAAAACTTTCAGCCACATCGTGCTTT TCAGCGTTCTCTTCCATTTGCTCCCCTAGTCGCTCTTCTGTGTTTGCCCTCTGCTCACCCAAACTGTGAG CTTCCTGATAATCAGGCCTATCCATTTCCCTCACCCTCCTCCCGCTCTGCTGACAGTTCTCTTAATTGAT TTCTCAGATCCCAGATGCAGTGACTGGTTACTACCTGAACCGTGCTGGCTTTGAGGCCTCAGACCCACGC ATAATTCGGCTCATCTCCTTAGCTGCCCAGAAATTCATCTCAGATATTGCCAATGATGCCCTACAGCACT GCAAAATGAAGGGCACGGCCTCCGGCAGCTCCCGGAGCAAGAGCAAGGACCGCAAGTACACTCTAACCAT GGAGGACTTGACCCCTGCCCTCAGCGAGTATGGCATCAATGTGAAGAAGCCGCACTACTTCACCTGAGCC ACCCAACCTAAATGTACTTATCTGTCCCCATGTCCCCACACCAGCCTGTTTTCATAATAAACTTTATTGT GACAGGCGGGGCTGATCCCTCCCATGTTGGGAGACACCATGTGGCAAGTGACAAAGCTCTGAGCCCGCCC CTCTTGGGGCCACAGTGGTAGGGATGGGGGAAGGGGATGGACCCCATGGCTGGGGTAGTACCATGACTGG AGGCGGGGGAGGCAACCAGAGGCCTGCTGCTTTGGGGAGGTGCATTCCCCCAACCATGTCCCGACACCTC TGGAGTTCAGGCAAGGACCTTCCAGTCCTACTTGTCCTGCATCTTCTCAAGGATAGGCACAATCATGTCA AATTTGGGTCGCTTTGCAGGGTCTTCATTCATGCAGATCTTCATGAGCTTACACACATGAGGGGAAATAC CTGGTGGGATGGTAGGCCGAAGGCCTTCCAATGCCACCTGCGAATACAAGAAAAAAAGCAGCCTCACAAT TTGAGTCTGTCATGTAGAGAGAAGCCACTGGCCCTTGGGACAGGATTACTGTATATCCGAACAAATATGT GAGGAGCCAAGGCCCACTACAGTTATTTTCACCATCACCAACCACTCCCGCAACACAAATGTATGCTGTT GTGCTCTCACCTTCATTCCAATCTCCATATTGGAGAGGTCAGCAAAGGGTACCTCCCGTGTCACCAGTTC CCACAGAAGCACTGCAAAACTCCACATGTCTGCTGAGCGTCTGTTTGTGTCTTCAGGCTTCTTCTGCAGA GCTGGAGAGATAGGGCTTGTCTGTTCTGGCCCCCTTGCCTGTCCTGTCTCTGCCCCCTTCCACTACTTCT GAGGTGGTCCTTTTTTACCTCCCGACATGATGACTTCACTCACCTTCGGGGGCTACCCAGGCAGGTGCAT ACATGCGACCAGGACATTGGAAAGAGAACTTGACATCAGCCATGCTAATTCGGGCAGTCATGTCCTCATC AATCTGAGGAAGAGAAAACAGATAGAAGGAGGTAAGTCATAGATAGGCAAAGGGCTTTTGGGGCCTGGGC CAGGAGTGAGCTTGTGGCCTCACCATTACACTACGGCTATTGAGTGCATGTCGTGGGATGAGGGGCTCTA GTGTGTGTAGGAAGGCCATGCCCCTTGCCATGTCCAAAGCAAACTTCACAGCCTGGCTCTGGTCCACGAC GAAATCTGAGAGGAGGCAAAAGTCAAATAGTTCAGAGAGGCTCCCTCTCTGCCATTTCCTCCCCAAGGAA ATTCAACATCCCTACTCACTGGTGCCTTCATGTAGTACATTGTAGAGGGATCCATACGGCATCCAGTGTG TGATGAGAGTAGGATGAGGAGCAGGTGGAGACTGGCAGGCACCTAGCACTGGGAGCACATTTGGATGCGA GAAAATCCTAGAAGAGGGAGGGAGTGGTTGAGACCCAGTACAAAAGATCTCTTTCAGTGCTATTCAGGGT TCCTTGGAACTAGCAGCTTCCAGGTTACGCCTTGCACTACCTGAGCCGGGGACACTCTTCATTGAAGTCC CTGCTCTTCCTTGTACTCCAGTCTCGAACCTTCAGCACCTTCACGACAATGTCATTGCCCTGCCAGCGGC CCTTCCATAGCTGCAGGACAGGCAGGGGTTAGGAGGCTTGAAATGTTGCTGCCACTTCCTTGCCCACTAA CTGGAGGAAAAACTTAAAACAGGTACAAAGCAAGTAATTTATGGGGGTTTAGTGAGGGAAGGGCAAGAAG GGCAGGGGTCACCTCTCCAGAGTGATTCTCGTTGAGCTTCGTCAGGAAGTTAAGCTGTTTGAAGTCAATG CCAGAGTGTTTGTTCAGGGTTCCATTTCCTAAGAGTGTGGGCAGGTCAGGTACCCAGCTTCACCCTAACC CAGCCCCAAGAGGCCAGGATTATTTCCTTTTACAACCCTTCTTCCCACAGTGGTGACTCACGGGGCCGAG TGCGGGTGGTCCCCTTCCAGAATGTGTCCTTGTATGGAATACGGTTGAGATTCTGGCCCATCTTCTCTGC CCGCTCTAGGGAAGAAAAACAACCTAAGCTGTGGTACAGAGGGAGAAGACAGGTGGGAAGGGACGAGAGG ACACAAGCTAGGGGATGGGGAGATGGACCTCGGAGAAGCTCTCTCAGGGGTGCCTTGGCTTTGTCCACAG GCATCTCTCCATACTTGTTACAGATGCTGACAAGGGCCCCATTTGCCACCAGGTCCTGGAATGAAAAGGC CATTATGATGAGAGCCACTGCTACCTCGCTGGCAGTCACTGAACCAACCATGAATGCACAAATGTAGAAC TTAATCCTGTAAGTGTCACACAGTAGTACTGACAGAATGGGTTGAGGGTTCCAAAATCTCCCACCAAGGC TCAGACTTTACTTCCTACCCATCTCAGGAATTAAGGGCTGAGTACTCACCTCTGCCACTTGATCTTGGCC CCAAAAACAGGCATAGTGCAGGGGCACATTCCCGTGTTCATTCACTGCATTGATGTCTGCCTTGTACTGC AATAGCTGGACCCCATGGCCAGACAAAAAGAAGAGGCAGAAAGTACAGTCAGTTCCAAATGAGATACTGG TGTCAGGGCATGGGAAAAGGGGGAAGAGCACACATGCTTTCTACTGTATGTTACAGTGCCTGTGACTGAC CCTCATGGCATGTATGTGATGTGGATGACGAAGGAGTTTGTACGTACCTTCTGTACAATATCACGGTGTC CATGACTGGCTGCCAGATGCAGGGGGGTGTCATCCCCACGGTTCATTACATTGATCCGTGCCCCCCGCAT GATCAACATCTCAACCACAGCAGAGCGGCCCTCTCGGCAGGCCCAGTGCAAGGGGGAGAAGCCATGATCG TCCCTTTGAGGAAGGGGCAATGGTCATTGAGCTGGAGGTGGGATGGGGCAAAGGTTTTGATTCCTGAAAA ACTGATAATTACATACTCTCTGGGAAGGCTTACCTCTCTAGGACTTTGATTCTCATTCATGCCAGCAACT CCCAAAGCTGTCCATTTCAGACCTTCCCCTGAGCTTCAAACCAAACCAACCACCTAGATATCTCAACAAC CTCTATAAAAAAATGTGTTATCTTCACCATATCCCACATCTCATTTCCTTAGGTGGTAGTGCTCTTCCCC TGGTTTCTCTTGCACCCCTTCTGCAACATCTATTGGTCCTTTCTGTTTTCCACCACATCTTATATGTCCC CCTACTTCAGCACTTGTTGCACTGGATTGTAAAAATCTGTTAATGGACTGTCTTGTCTGTTAGACCTTTA AGTTTCTTGAGGGAATGGATTGACAGCAGCTGTTCAGTGCATGTTAGTGGGGGAGAAGGGTGTCTTGGAG AAGGCAGGCCACAACTTATAGAAGAGGGAACAAACTTACTATTTGGCTCTGGTACTGAAACTAACATCTA AAAATGGAATGGAATTTAAATGATGTTTTGGAAGGGTTTATGTTGAAAAGGAGAAATGCTTTTGACTACT GATGAGGTAGATAACAAACCACCATGCAAAGCAGGATTCCCCTACTTAGCAATCCTGTGCATAGAAAACA GAAGAAAATGAGAATGGCTGTTTTGGGATTAAAACTATACCTGATTTTTTTCTTTTCCAAGAAATATGTA TTTTATAAAGTAAAGTATACAAACAAAACAAAACAATTGGTTTGGAGCTGGGCCTGCCTGACAGCCTGGG GCCATAAAAGGAAATTCCCTCCAGTTCGGGGCTGCAGATTCAAGGGGCGGAGCAGGGGGCGGTCCGTGGT CTGGGCCAGCAGCCTCCGGATGCCTTAGTGTTGTGGTCACTTCCTGTGTGCGACTCTAGGCAAGGCCTGG AGCTGATCAGGGGTCTGGCCATCCAGATGCCCGTACAGCTGAAGGGGTTGGGGGGTGTGGCCTTTAAGTT AATCATTTTCCTCCACAACTTGCTCGCATCTGCTAACACAGAAATAGCCAAAAGCTTTTCCTTGAGATGG GATTATCCCCAGGAAGCATTACGCCCCCTGTTTATCAGTTGGTCTTGCCTCCTAGACCTTTAATTTGTGC CCTGACTTATCAGCTTCCAGTACTGCCTGTGGCGCCAGGGCAGGCCAAACTCGGGGGGCAAAAGTATGGG CACAAGGGTTCTGTTCACTATCAGGCTGCCTGCCACCTCAGGGCCTGGACTTCTATCTCAAGAAGACCTG CAAGGAATTAACCCCCTCTTCATATCCAGCATATAGCCAACTTTGGCCCCCATACTTTACTCCTCAAACC AGCACCACAACCTTCCAGTTTCATTAATTCCACCCCTTTTCCTTCTGGCAGCATCTTCCTAGAGTCTTCC TAGACTCAGCTAGAGTCTTCCTAGATGCTAGGCCCACTGGGCAACTGCCTAAGCCTCTATTTCCTCGCCA ACAAAACAGTAACAGTGCTTACTGTCTTTATCAGGGTACCCTTTGAGAATTATATGAAAAATGGAACAAA TGACTTTGCAAAGTATTAAAGAGAAATAGTGTAGAACCTAATGGGAACCAGTGAAGTCTAGACTAAGATG ACAGGTGATCCTGACCTGTCAAACCTAAAAACTAAACTCCACCAATTTTAAAAAATAAAGAATTTGATTT TGCTAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL833496 unedited CAAAATTTGTAATACGACTCACTATAGGGCGGCCGCGAAATCGGCACGAGGCCCACGCAT AATTCGGCTCATCTCCTTAGCTGCCCANAAATTCATCTCAGATATTGCCAATGATGCCCT ACAGCACTGCAAAATGAAGGGCACGGCCTCCGGCAGCTCCCGGAGCAAGAGCAAGGACCG CAAGTACACTCTAACCATGGAGGACTTGACCCCTGCCCTCAGCGAGTATGGCATCAATGT GAAGAAGCCGCACTACTTCACCTGAGCCACCCAACCTAAATGTACTTATCTGTCCCCATG TCCCCACACCAGCCTGTTTTCATAATAAACTTTATTGTGACAGGCGGGGCTGATCCCTCC CATGTTGGGAGACACCATGTGGCAAGTGACAAAGCTCTGAGCCCGCCCCTCTTGGGGCCA CAGTGGTAGGGATGGGGGAAGGGGATGGACCCCATGGCTGGGGTAGTACCATGACTGGAG GCGGGGGAGGCAACCAGAGGCCTGCTGCTTTGGGGAGGTGCATTCCCCCAACCATGTCCC GACACCTCTGGAGTTCAGGCAAGGACCTTCCAGTCCTACTTGTCCTGCATCTTCTCAAGG ATAGGCACAATCATGTCAAATTTGGGTCGCTTTGCAGGGTCTTCATTCATGCAGATCTTC ATGAGCTTACACACATGAGGGGAAATACCTGGTGGGATGGTANNGCCGAAGCCTTCCATG CCACCTGCGAATACAAGAAAAAAAGCAGCCTCACAATTTGAGTCTGTCATGTAGAGAAGA GCCACTGGCCCCTTGGACANGATTACTGTATATCCGAAACAATATGTGAGGAGCCAAGGC CCACTACAGTTATTTTCACCATCACCAACCACTNCCGCACACAAATGTATNGCTGNTGTG CTCTCACCTTCATTNCATCTNCTATTNGNAGAGTCAGCAAGGGTACCTCCGTGTCCCAGT TCCACAAAGCCTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL833496 unedited CATGTNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTGCAAAT TCAAATTCTTTATTTTTTAAAATTGGTGGAGTTTAATTTTTAGGTTTGACAGGTCAGGAT CACCTGTCATCTTAGTCTAGACTTCACTGGTTCCCATTAGGTTCTACACTATTTCTCTTT AATACTTTGCAAAGTCATTTGTTCCATTTTTCATATAATTCTCAAAGGGTACCCTGATAA AGACAGTAAGCACTGTTACTGTTTTGTTGGTGAGGAAATAAAGGCTTATGCAGTTGCCCA GTGGGCCTAGCATCTAGGAAGACTCTAGCTGAGTCTAGGAAGACTCTAGGAAGATGCTGC CAGAAGGAAAAGGGGTGGAATTAATGAAACTGGAAGGTTGTGGTGCTGGTTTGAGGAGTA AAGTATGGGGGCCAAAGTTGGCTATATGCTGGATATGAAGAGGGGGTTAATTCCTTGCAG GTCTTCTTGAGATAGAAGTCCACGCCCTGAGGTGGCAGGCAGCCTGATAGTGAACAGAAC CCTTGTGCCCATACTTTTGCCCCCCGAGTTTGGCCTGCCCTGGCGCCACATGCCGTACTG GAAGCTGATAAGTCAGGGCACAAATTAAAGGTCTATGAAGCAAGACCCACTGATAAACAA GGGGCGTAATGCTTCCTGGGGATAATCCCATCTCAGGAAAAGCTTTGGCTATTTCTGTGT AGCAGATGCGAGCCAGTTGCGGACGGAAATGATTAACTTAAGGGCCCCCCCTCCAACCCC TCAACTGGCCGGCATTTGGATGGCCAAACCCTGGACAGTTCCAGGCCTTGCCTAAATCCC CCCCGGGAAGGGCCCCCCCCTCAAGAATCCCGGGGCTGCTGGGCCAAACCAAGAACCCCC CCGGTTCCCCCTTTGAATTTGGCCCCCAACTGGAGGAAATCCT
Restriction Sites	NotI-NotI
ACCN	AL833496
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL833496.1
RefSeq Size	5413 bp
RefSeq ORF	5413 bp
Locus ID	3611
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Endometrial cancer, Focal adhesion, PPAR signaling pathway
Gene Summary	This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

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