C8orf58 (BC012750) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ34715
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC012750, the custom clone sequence may differ by one or more nucleotides ATGGCAGTTGGGGACAGCCCCCTGGCCGCCTTACCGGGCCTTTCTCAGGACTCCCTGGACTTTGAATCCT CAGGGAGTTCTGAGCCCCCCGCCCAGGTAGGCCGACTCCTGGCCAGCCAGAAGCTGGGGGAGGTGTTGGA GCGGTCCCGCCGGCTCCCAACAGCTCCCACCAGCTTGTCAGGACAACACCGCTCCCTGCGGCTGGCAAGC AAGCCTGAGCGTGAAGTGCCCCTTGGAGCAGGGCAACAGGAGTCCATGGAGGCAGACACAGACCTAGAGG CAGGCCTGGAAGAAGAGGCGGTGGGGGGCCTGGGGCCTGGAGCCTGGGCCTGCCTCCCCGGGCAGGGTCT TCGCTATCTGGAACACCTGTGCCTGGTGCTGGAGCAGATGGCAAGGCTCCAGCAGCTCTACCTGCAGCTG CGGATCCAGAGGCCCCCAGGGGATCCCGGCGAGGAGGAGTCGACCCGAGCCCCTTTACCGTCCCCGTTAC ACACCCCAGGCAATCGGGGGCAGGGGCCATGGGAGCTGCTAAGCCAGACAGAGCACACAGGAGCAAAGGC TGCTTCACCCCCAAAGGTGGAGGTGCCCAGTGCCAACCCTCCCAGGCTGCCAGAAACCCCAGTGGAGCCA ACGTACCACTTGCCATCCTCCCAGGGACACAAGCGGGATATCTCCCACTGGGACAAGGTCAAGGTCCTGC TCAACCGGATCTGCCGGAGAAGCCACCACCACCCTGAGCCCCCTGCCCCTCCTGATGGCTCTGACCCCAG GATCGAGTCCAGGGACCTCCCTGAAAGGCCTCAGTGCCGCCCCCACCGGAAGACCTTTATGCCATCATTA GTGGTTAAGAAGCAACGAGCAAAAAAACCTTTCTGTAGGCTGAGACCTCTCGGTGCACCTGGTGACCCTG GGTGGAGGGGACTTGCTGTGAAGTCTTCCTCGCCCTCTGCCCTCTTGCTGCTTCTCCACATTGCCAGGAA AAGCTGCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012750 unedited AATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCAGCACCTACAGAC GGATCCCCNCGCTGCCCACGGGTGCTCATCCTGGGAGAGAGGTGACAAGTTCAGAGGTGT CGGCAGGGAGGCACTCTTTCTCAAACTGGCCTCCCGGGACTCAGGAGTGGAGATGGCAGT TGGGGACAGCCCCCTGGCCGCCTTACCGGGCCTTTCTCAGGACTCCCTGGACTTTGAATC CTCAGGGAGTTCTGAGCCCCCCGCCCAGGTAGGCCGACTCCTGGCCAGCCAGAAGCTGGG GGAGGTGTTGGNAGCGGTCCCGCCGGCTCCCAACAGCTCCCACCAGCTTGTCAGGACAAC ACCGCTCCCTGCGGCTGGCAAGCAAGCCTGAGCGTGAAGTGCCCCTTGGAGCAGGGCAAC AGGAGTCCATGGAGGCAGACACAGACCTAGAGGCAGGCCTGGAAGAAGAGGCGGTGGGGG GCCTGGGGCCTGGAGCCTGGGCCTGCCTCCCCGGGCAGGGTCTTCGCTATCTGGAACACC TGTGCCTGGTGCTGGAGCAGATGGCAAGGCTCCAGCAGCTCTACCTGCAGCTGCGGATCC AGAGGCCCCCAGGGGGATCCCGGCGAGGGAGGAGTCGACCCGAGCCCCTTTACCGTCCCC GTTACACACCCCAGGCAATCGGGGGGCAGGGGCCATGGGAAGCTGGTTAGCCAGACAGAG CACACAGGAGCAAAGGCTGGCTCACCCCCAAGGTGGGAGGGGGCCCAGTGCCAACCCTTC CCAGGCTGCCAGAACCCCCGGGGGGGCCCACGTACCACTTGCCATCCTTCCAGGGACACC AGGGACCGGGTCCAGGGGCCCTCCCTGAAGGGGCTCAATGGCCGGCCCCACCGGGAGACC C 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC012750 unedited GTCTATGNNACCGCGGCCGCAATCTANNATCGAGTTTTTTTTTTTTTTTTTTGGACAGGA ATCTTGCTTTGTTGCCCATGCTGGAGCACAGAGGCAGCTAGCTCAGGTCATTACTATGTC CAGCCTGCTGGGTTCACGCCAGACATGTGCCTTAGCCTTCTAAGTACCTGGGACTACAGG TGCCTTGCCATCAAGCCCTGTTAACTCTTTTCGGATTTTTAGTAGCAGAGCACTGGCTTT GAAAGTTATTCAATTCCCAGGTTGTCCCTGATCTTCCTCATTCCTTGTGCAGTCTTCAAG AGTCCGGGGCAAGATTCCATGCAGAGGCAGCCAAAGCCACACCGTTCTCTGTCCCAGGGC TGCCAGCATGGCTCTGAGGAGGAAAGCTCATTCACTGCCCACTGGGGAATAAAGGTCAGT TCCATCCTTCTGCAGTGACCTCCCCCAGAATATCAACCCTTTGGATGCATTATTTCACAA AAGATGGTTGGATCCAGCCAGCTTGCAACACTGACAGTCACACAGAGATATGCATACCAT TGCGCATAAATACACATCTGCAACATGCTGGGCCCAGACAGGTTACCATGCCCAAAACAC TTCACGGACACCTGACAATGAGTGAGTTCACATTGTGGAAGCCCACAGATTTCTTGTGAG GAAGAAGAGCCCCTGGAGCTGCNGCATCTTGGGGGCCTCTCAAGAAACCAAAGGTCCTGA TCCGACTGTTTTTAGGAGAAGAATCAACCCTCCCCTCAGAGAGAAGGCATGCGTCACAGC CTTTCTGGCATGTGGAAAACACCAGAGGCAGAAGGCCAGGAAAACTCCGCTAGTTCCCTC CGCCGGGGTCCCCGTGCCCCCGGGTCTCACCCACCAAAGGTTTTGCCCCTGTTTTTACCC CTATATGCGTAAGGCTTTCCGGGGGCCGGCTTGGCCCTCA
Restriction Sites	NotI-NotI
ACCN	BC012750
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012750.2, AAH12750.1
RefSeq Size	1978 bp
RefSeq ORF	990 bp
Locus ID	541565

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