PRKCBP1 (ZMYND8) (BC001004) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC001004, the custom clone sequence may differ by one or more nucleotides AAAAAAGAAAAAGAAAAACAATTAAAAAAAAAAAAAAAAAAGCAACCAACCCCAACAACAAAAAAGAATG TTTTGGTATTGGAGAAGGGATGGTCAGTTAGCCTGTCTGTCACACGACGGAATGGATACTGGGCCCGGGG ACCACTTTCATACTCACGTCCTCATCCTTGGATACCCAGGGGAGGGCGAACCGTTTTCGCTCGTGTGTCT GTACGCAGCATGTTGGGATCGGGAGTTTCGGCACAGACTATCCCATCAAGCCGTTGGCTCCTTTCAGCTA CTACGTTACCACGTTCCTAAAACGCAAGCTCTCCGGACCAGACGGACACAGGGAGAAGCTAGTTTCTTTC ATGTGATTGAAATGATGACTCTACTCCTAAAAGGGAAAAAACAATATCCTTGTTTACAGAAGAGAAACAA ACAAGCCCCACTCAGCTCAGTCACAGGAGAGAACACAGAAAGTCTTAGGATCATGAACTCTGAAAAAAAG AGAAACCTTATCTTTGCTTTGTGGTTCCTTTAAACACACTCACACACACTTGGTCAGAGATGCTGTGCTT CTTGGAAGCAAGGACTCAAAGGCAAGGTGCACGCAGAGGACGTTTGAGTCTGGGATGAAGCATGTACGTA TTATTTATATGATGGAATTTCACGTTTTTATGTAAGCATGAAACACAGGCAGTATGAGAGAAAGCAAGGC CCGTCATGCTGTCCGTACACTACGTATGCTGTAGAGCCATTTTGTATGTTGTGTAAAACAAAAAGCATTG ATGAAAAAGCAAAAGGTGATGTATGTATATGAGAAAATTAATTGTACGATATCATTCCAGTACGTTTTGT TGTACATTTTAGTCTTGTTTACTTTCTCTTCATTGTTAAGAGGATGCGAACTGTACAGTTTCCAGCTAGT TACCCATATTAGAGAAGAAATAAGAGAGTATTAGAAGAAAACAGGAGAGAAAGAACATTTGTGAATTGCA GTTGTCAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001004 unedited CTATAGGCGGCCGCGNAATTCGCACGAGGAAAGAATGTTTTGGTATTGGAGAAGGGATGG TCAGTTAGCCTGTCTGTCACACGACGGAATGGATACTGGGCCCGGGGACCACTTTCATAC TCACGTCCTCATCCTTGGATACCCAGGGGAGGGCGAACCGTTTTCGCTCGTGTGTCTGTA CGCAGCATGTTGGGATCGGGAGTTTCGGCACAGACTATCCCATCAAGCCGTTGGCTCCTT TCAGCTACTACGTTACCACGTTCCTAAAACGCAAGCTCTCCGGACCAGACGGACACAGGG AGAAGCTAGTTTCTTTCATGTGATTGAAATGATGACTCTACTCCTAAAAGGGAAAAAACA ATATCCTTGTTTACAGAAGAGAAACAAACAAGCCCCACTCAGCTCAGTCACAGGAGAGAA CACAGAAAGTCTTAGGATCATGAACTCTGAAAAAAAGAGAAACCTTATCTTTGCTTTGTG GTTCCTTTAAACACACTCACACACACTTGGTCAGAGATGCTGTGCTTCTTGGAAGCAAGG ACTCAAAGGCAAGGTGCACGCAGAGGACGTTTGAGTCTGGGATGAAGCATGTACGTATTA TTTATATGATGGAATTTCACGTTTTTATGTAAGCATGAAACACAGGCAGTATGAGAGAAA AGCAGGCCCGTCATGCTGTCCGTACACTACGTATGCTGTAGAGCCATTTTGTATGTTGTG TAAAACAAAAGCATTGATGAAAAAGCAAAGGTGATGTATGTATATGAGAAAATTAATTGT CGATATCATTCCAGNCCGTTNTGGTGGACATTTTTAGACTTGTTTACTTTTTCTTCATGG TAAGAGGATGCGAACTGTACAGGTTCCAGCTTGTTACCCTATTAAAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC001004 unedited NNCCGATATATCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTATTTTTCCAAAACAGTTTAATTAAAAAAAGTAAAGAAA TCTGAAAAAACTGGGGGGGTGGGGGAAGGAACGGGCAAACCCAAAAAAGCATATAGTGTG AGTCTACTGGCTCAGTCACAAAGAAACACCCCCCTCAAAAAGATATTTAAGTTTAATACA AATTTTATACCAAGAAAATGTGAAAAAATACTTCCATATGCTAAAAGCAATTATGCTTCA CAAATAAGGCCAGCTAGGCTATTTTTTTTTTTGACAACTGCAATTCACAAATGTTCTTTC TCTCCTGTTTTCTTCTAATACTCTCTTATTTCTTCTCTAATATGGGTAACTAGCTGGAAA CTGTACAGTTCGCATCCTCTTAACAATGAAGAGAAAGTTAACCAGACTTAAATGTTCAAC AAAACGTACTGGAATGATATCGTACAATTAATTTTCTCATATACATACATCACCTTTTGC TTTTTCATCAATGCTTTTTGTTTTACACAACATACAAAATGGCTCTACAGCATACCTAGT GTACGGACAGCATGACGGGCCTTGCTTTCTCTCATACTGCCTGTGTTTCATGCTTACATA AAAACGTGAAATTCCCTCCTATAAATAATACGTACATGCTTCATCCCAGAATCAAACGTC CTCTGGGTGCCCCTTGCCTTTGAGTCCCTGGTTCCAAAAAGCACAACATCTTTGACCAAA TGTGTGTGAATGTTGTTTAAGGGACCCCNAAGCCCAAATAAGGTTTTCCTTTTTTTCCAA GTTTAGAATCCCAAACTTTTTGGGTCTCCTCCGGGACTGAACTAAAGGGGC
Restriction Sites	NotI-NotI
ACCN	BC001004
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001004.1
RefSeq Size	1002 bp
RefSeq ORF	1002 bp
Locus ID	23613
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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