Iduronate 2 sulfatase (IDS) (AF050145) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MPS2; SIDS
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF050145, the custom clone sequence may differ by one or more nucleotides ATGCCGCCACCCCGGACCGGCCGAGGCCTTCTCTGGCTGGGTCTGGTTCTGAGCTCCGTCTGCGTCGCCC TCGGATCCGAAACGCAGGCCAACTCGACCACAGATGCTCTGAACGTTCTTCTCATCATCGTGGATGACCT GCGCCCCTCCCTGGGCTGTTATGGGGATAAGCTGGTGAGGTCCCCAAATATTGACCAACTGGCATCCCAC AGCCTCCTCTTCCAGAATGCCTTTGCGCAGCAAGCAGTGTGCGCCCCGAGCCGCGTTTCTTTCCTCACTG GCAGGAGACCTGACACCACCCGCCTGTACGACTTCAACTCCTACTGGAGGGTGCACGCTGGAAACTTCTC CACCATCCCCCAGTACTTCAAGGAGAATGGCTATGTGACCATGTCGGTGGGAAAAGTCTTTCACCCTGGT ACTGCTCCATGTCCAGAGTCTGGGTTCTCTTGGTTTGTGGTGTCTGAATCCAGCATTCCCATCCTGGGGA TGGGGCTGTCTTTGCAGAGCCCTCTTCTGGCTGGGCGAGTCCCTCGCTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF050145 unedited GGGCGGCCCGCGAATTCGCACGAGGGTCGCACGGACGCACTCGCGCTGCGGCCAGCGCCC GGGCCTGCGGGCCCGGGCGGCGGCTGTGTTGCGCAGTCTTCATGGGTTCCCGACGAGGAG GTCTCTGTGGCTGCGGCGGCGGCTGCTAACTGCGCCACCTGCTGCAGCCTGTCCCCGCCG CTCTGAAGCGGCCGCGTCGAAGCCGAAATGCCGCCACCCCGGACCGGCCGAGGCCTTCTC TGGCTGGGTCTGGTTCTGAGCTCCGTCTGCGTCGCCCTCGGATCCGAAACGCAGGCCAAC TCGACCACAGATGCTCTGAACGTTCTTCTCATCATCGTGGATGACCTGCGCCCCTCCCTG GGCTGTTATGGGGATAAGCTGGTGAGGTCCCCAAATATTGACCAACTGGCATCCCACAGC CTCCTCTTCCAGAATGCCTTTGCGCAGCAAGCAGTGTGCGCCCCGAGCCGCGTTTCTTTC CTCACTGGCAGGAGACCTGACACCACCCGCCTGTACGACTTCAACTCCTACTGGAGGGTG CACGCTGGAAACTTCTCCACCATCCCCCAGTACTTCAAGGAGAATGGCTATGTGACCATG TCGGTGGGAAAAGTCTTTCACCCTGAGATATCTTCTAACCATACCGATGATTCTCCGTAT AGCTAGTCTTTTCCACCTTATCATCCTTCCTCTGAGAGTATGAAACACTAAAACTGTCGA GGCCAGATGAGAACTCCTGCAACCTGCTTGCCCTGGGATGGCTGGATGTCCCGAGCCACT TGCCTGACAACAGACACTGAGCAGCCATACAGTGTTGGAAAAAAGAAACGTNACCAGTCC TTTCTTCTGCCCGTGGTATATAAGCACCCTCCCTTCAGAACCCAAGAN
Restriction Sites	NotI-NotI
ACCN	AF050145
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF050145.1, AAC05984.1
RefSeq Size	1098 bp
RefSeq ORF	1098 bp
Locus ID	3423
Protein Families	Druggable Genome
Protein Pathways	Glycosaminoglycan degradation, Lysosome, Metabolic pathways
Gene Summary	This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

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