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USP44 (NM_032147) Human Untagged Clone
CAT＃: SC106025
USP44 (untagged)-Human ubiquitin specific peptidase 44 (USP44), transcript variant 1
CNY 7,832.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC106025 sequence for NM_032147 edited (data generated by NextGen Sequencing)
ATGCTAGCAATGGATACGTGCAAACATGTTGGGCAGCTGCAGCTTGCTCAAGACCATTCC AGCCTCAACCCTCAGAAATGGCACTGTGTGGACTGCAACACGACCGAGTCCATTTGGGCT TGCCTTAGCTGCTCCCATGTTGCCTGTGGAAGATATATTGAAGAGCATGCACTCAAGCAC TTTCAAGAAAGCAGTCATCCTGTTGCATTGGAGGTGAATGAGATGTACGTTTTTTGTTAC CTTTGTGATGATTATGTTCTGAATGATAACACAACTGGAGACCTGAAGTTACTACGACGT ACATTAAGTGCCATCAAAAGTCAAAATTATCACTGCACAACTCGTAGTGGGAGGTTTTTA CGGTCCATGGGTACAGGTGATGATTCTTATTTCTTACATGACGGTGCCCAATCTCTGCTT CAAAGTGAAGATCAACTGTATACTGCTCTTTGGCACAGGAGAAGGATACTAATGGGTAAA ATCTTTCGAACATGGTTTGAACAATCACCCATTGGAAGAAAAAAGCAAGAAGAACCATTT CAGGAAAAAATAGTAGTAAAAAGAGAAGTAAAGAAAAGACGGCAGGAATTGGAGTATCAA GTTAAAGCAGAATTGGAAAGTATGCCTCCAAGAAAGAGTTTACGTTTACAAGGGCTCGCT CAGTCGACCATAATAGAAATAGTTTCTGTTCAGGTGCCAGCACAAACGCCAGCATCACCA GCAAAAGATAAAGTACTCTCTACCTCAGAAAATGAAATATCTCAAAAAGTCAGTGACTCC TCAGTTAAACGAAGGCCAATAGTAACTCCTGGTGTAACAGGATTGAGAAATTTGGGAAAT ACTTGCTATATGAATTCTGTTCTTCAGGTGTTGAGTCATTTACTTATTTTTCGACAATGT TTTTTAAAGCTTGATCTGAACCAATGGCTGGCTATGACTGCTAGCGAGAAGACAAGATCT TGTAAGCATCCACCAGTCACAGATACAGTAGTATATCAAATGAATGAATGTCAGGAAAAA GATACAGGTTTTGTTTGCTCCAGACAATCAAGTCTGTCATCAGGACTAAGTGGTGGAGCA TCAAAAGGTAGAAAGATGGAACTTATTCAGCCAAAGGAGCCAACTTCACAGTACATTTCT CTTTGTCATGAATTGCATACTTTGTTCCAAGTCATGTGGTCTGGAAAGTGGGCGTTGGTC TCACCATTTGCTATGCTACACTCAGTGTGGAGACTCATTCCTGCCTTTCGTGGTTACGCC CAACAAGACGCTCAGGAATTTCTTTGTGAACTTTTAGATAAAATACAACGTGAATTAGAG ACAACTGGTACCAGTTTACCAGCTCTTATCCCCACTTCTCAAAGGAAACTCATCAAACAA GTTCTGAATGTTGTAAATAACATTTTTCATGGACAACTTCTTAGTCAGGTTACATGTCTT GCATGTGACAACAAATCAAATACCATAGAACCTTTCTGGGACTTGTCATTGGAGTTTCCA GAAAGGTATCAATGCAGTGGAAAAGATATTGCTTCCCAGCCATGTCTGGTTACTGAAATG TTGGCCAAATTTACAGAAACTGAAGCTTTAGAAGGAAAAATCTACGTATGTGACCAGTGT AACTCAAAGCGTAGAAGGTTTTCCTCCAAACCAGTTGTACTCACAGAAGCCCAGAAACAA CTTATGATATGCCACCTACCTCAGGTTCTCAGACTGCACCTCAAACGATTCAGGTGGTCA GGACGTAATAACCGAGAGAAGATTGGTGTTCATGTTGGCTTTGAGGAAATCTTAAACATG GAGCCCTATTGCTGCAGGGAGACCCTGAAATCCCTCAGACCAGAATGCTTTATCTATGAC TTGTCCGCGGTGGTGATGCACCATGGGAAAGGATTTGGCTCAGGGCACTACACTGCCTAC TGCTATAATTCTGAAGGAGGGTTCTGGGTACACTGCAATGATTCCAAACTAAGCATGTGC ACTATGGATGAAGTATGCAAGGCTCAAGCTTATATCTTGTTTTATACCCAACGAGTTACT GAGAATGGACATTCTAAACTTTTGCCTCCAGAGCTCCTGTTGGGGAGCCAACATCCCAAT GAAGACGCTGATACCTCGTCTAATGAAATCCTTAGCTGA Clone variation with respect to NM_032147.2 >OriGene 5' read for NM_032147 unedited
TGTATACGACTCATAAAGGCGGCCGCGACATTCGCACGAGGCCCGGCTCGGGCTGTTTTC AGATGCTTCAAGTGTTGTGAACAGAGACTTGTTTGGATTATGCATTTCTCAGCTAGACTA AATAAATGCTAGCAATGGATACGTGCAAACATGTTGGGCAGCTGCAGCTTGCTCAAGACC ATTCCAGCCTCAACCCTCAGAAATGGCACTGTGTGGACTGCAACACGACCGAGTCCATTT GGGCTTGCCTTAGCTGCTCCCATGTTGCCTGTGGAAGATATATTGAAGAGCATGCACTCA AGCACTTTCAAGAAAGCAGTCATCCTGTTGCATTGGAGGTGAATGAGATGTACGTTTTTT GTTACCTTTGTGATGATTATGTTCTGAATGATAACACAACTGGAGACCTGAAGTTACTAC GACGTACATTAAGTGCCATCAAAAGTCAAAATTATCACTGCACAACTCGTAGTGGGAAGG GTTTTTACGGTCCATGGGTACAGGTGATGATTCTTATTTCTTACATGACGGTGCCCAATC TCTGCTTCAAAGTGAAGATCAACTGTATACTGCTCTTTGGCACAGGAGAAGGATACTAAT GGGTAAAATCTTTCGAACATTGGTTTGAACAATCACCCATTTGGAAGAAAAAAGCAAGAA GAACCATTTCAGGAANAAATAGTAGTAAAAGGAGAAGNTAAGAAAAGACGGCAGGAATTT GGAGNTATCAAGTTAAAGCAGAATTGGNAAAGTATGCCTCAANAAAGAAANTTTACGTTT ACAAGGGGCTCGCTCAGTCGACATATAGAAATNNTTTNTTGTTTGTTGAGGTGCCAGCAC AAACGCCAGCATCACCACAAAGGATTAAGTACTCTCTACCTCAGAAATGAATATNTCAAA AAAGTCGG >OriGene 3' read for NM_032147 unedited
NTTTAGCTTTGGACCGGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTAATG CTCAGGGCCAGTGTTTTAAGAGGCTCCTACCTAGTAGTCACATAATCCTAGCAAGAGTAA ATCAAGCTTTAGTTATTTAGAATGAGATGCTAGACATAATAATAGTCCAGTTGCCAATTT TGAACACTTATCTTTTCTGCCCTATAATTTTTTCTCTTCAAGACTCTATTCCTTTGAACT AAAAAAAATACATAAAGTTTTAAGAGATCTAATGTCATCAGACATTATAAAATGAACTCA TACCCAAGGTAAACTATTTGAATAACATCATACACTGGTTTACTAAATTCAGACTTTTTA TGACTGCTTAATTATTAGACTTCTGGACAAACCTGTTTGCTGCCAGACAAAAATCATAAA TCCCAGCCATCATTTTTTTCATGTCCATAGTTCCAGAGTTCACTTAAATATACTATTTGT AAAGCTGCCATTTTCATTTATAGTTTGACTATGCCAAAGAGCAATTACTAAAGCAATTNT TGGGGTTACATTAACCAAAATAATGTCAATTACAAGGCCTGTTAACACACAAAGCCTCAG AAATACCAGTGAGTGTGACTGAAGATATTTAGGTGATACCTTGACATGTCACTTCAATAA CTCCAAGGGNGCAAGGTAACTTCAATATACANATGAAAGACTATTAGTGACCAAAGTAGA GTCTAATTTTTCATCTATTAAGAATTTGTATAGAAAATACCTGAAAAAGAACATGTATAT AAAATGATGCCACTTGACTTTTGTTGGTAGAAGATTGCAACAGTTCATCATCCGAGCCCA TTTAGACATANANTTTTANNAGAGGGTACCTCNGTCCTGAGGTAACACCCAAAGTTTAAA ACTTNCAATATGAAAAAAGAAGTAACTCTCAGTGGATTTTACATTAAATACTTGTAAAAA AAAAAATGTTGATATTAAAGGATAAAGTGGTATACCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032147 |
| Insert Size | 3080 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032147.1, NP_115523.1 |
| RefSeq Size | 2871 bp |
| RefSeq ORF | 2139 bp |
| Locus ID | 84101 |
| UniProt ID | Q9H0E7 |
| Domains | UCH, zf-UBP |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome. [provided by RefSeq, Dec 2016] Transcript Variant: This variant (1) encodes the same protein as variants 2, 3, 5, and 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205560 | USP44 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 44 (USP44), transcript variant 1 |
CNY 7,824.00 |
|
| RC205560L1 | Lenti ORF clone of Human ubiquitin specific peptidase 44 (USP44), transcript variant 1, Myc-DDK-tagged |
CNY 10,224.00 |
|
| RC205560L2 | Lenti ORF clone of Human ubiquitin specific peptidase 44 (USP44), transcript variant 1, mGFP tagged |
CNY 10,224.00 |
|
| RC205560L3 | Lenti ORF clone of Human ubiquitin specific peptidase 44 (USP44), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205560L4 | Lenti ORF clone of Human ubiquitin specific peptidase 44 (USP44), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG205560 | USP44 (tGFP-tagged) - Human ubiquitin specific peptidase 44 (USP44), transcript variant 1 |
CNY 9,424.00 |
