USP44 (NM_032147) Human Untagged Clone

CAT#: SC106025

USP44 (untagged)-Human ubiquitin specific peptidase 44 (USP44), transcript variant 1



  "NM_032147" in other vectors (6)

CNY 7,832.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
USP44 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC106025 sequence for NM_032147 edited (data generated by NextGen Sequencing)
ATGCTAGCAATGGATACGTGCAAACATGTTGGGCAGCTGCAGCTTGCTCAAGACCATTCC
AGCCTCAACCCTCAGAAATGGCACTGTGTGGACTGCAACACGACCGAGTCCATTTGGGCT
TGCCTTAGCTGCTCCCATGTTGCCTGTGGAAGATATATTGAAGAGCATGCACTCAAGCAC
TTTCAAGAAAGCAGTCATCCTGTTGCATTGGAGGTGAATGAGATGTACGTTTTTTGTTAC
CTTTGTGATGATTATGTTCTGAATGATAACACAACTGGAGACCTGAAGTTACTACGACGT
ACATTAAGTGCCATCAAAAGTCAAAATTATCACTGCACAACTCGTAGTGGGAGGTTTTTA
CGGTCCATGGGTACAGGTGATGATTCTTATTTCTTACATGACGGTGCCCAATCTCTGCTT
CAAAGTGAAGATCAACTGTATACTGCTCTTTGGCACAGGAGAAGGATACTAATGGGTAAA
ATCTTTCGAACATGGTTTGAACAATCACCCATTGGAAGAAAAAAGCAAGAAGAACCATTT
CAGGAAAAAATAGTAGTAAAAAGAGAAGTAAAGAAAAGACGGCAGGAATTGGAGTATCAA
GTTAAAGCAGAATTGGAAAGTATGCCTCCAAGAAAGAGTTTACGTTTACAAGGGCTCGCT
CAGTCGACCATAATAGAAATAGTTTCTGTTCAGGTGCCAGCACAAACGCCAGCATCACCA
GCAAAAGATAAAGTACTCTCTACCTCAGAAAATGAAATATCTCAAAAAGTCAGTGACTCC
TCAGTTAAACGAAGGCCAATAGTAACTCCTGGTGTAACAGGATTGAGAAATTTGGGAAAT
ACTTGCTATATGAATTCTGTTCTTCAGGTGTTGAGTCATTTACTTATTTTTCGACAATGT
TTTTTAAAGCTTGATCTGAACCAATGGCTGGCTATGACTGCTAGCGAGAAGACAAGATCT
TGTAAGCATCCACCAGTCACAGATACAGTAGTATATCAAATGAATGAATGTCAGGAAAAA
GATACAGGTTTTGTTTGCTCCAGACAATCAAGTCTGTCATCAGGACTAAGTGGTGGAGCA
TCAAAAGGTAGAAAGATGGAACTTATTCAGCCAAAGGAGCCAACTTCACAGTACATTTCT
CTTTGTCATGAATTGCATACTTTGTTCCAAGTCATGTGGTCTGGAAAGTGGGCGTTGGTC
TCACCATTTGCTATGCTACACTCAGTGTGGAGACTCATTCCTGCCTTTCGTGGTTACGCC
CAACAAGACGCTCAGGAATTTCTTTGTGAACTTTTAGATAAAATACAACGTGAATTAGAG
ACAACTGGTACCAGTTTACCAGCTCTTATCCCCACTTCTCAAAGGAAACTCATCAAACAA
GTTCTGAATGTTGTAAATAACATTTTTCATGGACAACTTCTTAGTCAGGTTACATGTCTT
GCATGTGACAACAAATCAAATACCATAGAACCTTTCTGGGACTTGTCATTGGAGTTTCCA
GAAAGGTATCAATGCAGTGGAAAAGATATTGCTTCCCAGCCATGTCTGGTTACTGAAATG
TTGGCCAAATTTACAGAAACTGAAGCTTTAGAAGGAAAAATCTACGTATGTGACCAGTGT
AACTCAAAGCGTAGAAGGTTTTCCTCCAAACCAGTTGTACTCACAGAAGCCCAGAAACAA
CTTATGATATGCCACCTACCTCAGGTTCTCAGACTGCACCTCAAACGATTCAGGTGGTCA
GGACGTAATAACCGAGAGAAGATTGGTGTTCATGTTGGCTTTGAGGAAATCTTAAACATG
GAGCCCTATTGCTGCAGGGAGACCCTGAAATCCCTCAGACCAGAATGCTTTATCTATGAC
TTGTCCGCGGTGGTGATGCACCATGGGAAAGGATTTGGCTCAGGGCACTACACTGCCTAC
TGCTATAATTCTGAAGGAGGGTTCTGGGTACACTGCAATGATTCCAAACTAAGCATGTGC
ACTATGGATGAAGTATGCAAGGCTCAAGCTTATATCTTGTTTTATACCCAACGAGTTACT
GAGAATGGACATTCTAAACTTTTGCCTCCAGAGCTCCTGTTGGGGAGCCAACATCCCAAT
GAAGACGCTGATACCTCGTCTAATGAAATCCTTAGCTGA

Clone variation with respect to NM_032147.2
>OriGene 5' read for NM_032147 unedited
TGTATACGACTCATAAAGGCGGCCGCGACATTCGCACGAGGCCCGGCTCGGGCTGTTTTC
AGATGCTTCAAGTGTTGTGAACAGAGACTTGTTTGGATTATGCATTTCTCAGCTAGACTA
AATAAATGCTAGCAATGGATACGTGCAAACATGTTGGGCAGCTGCAGCTTGCTCAAGACC
ATTCCAGCCTCAACCCTCAGAAATGGCACTGTGTGGACTGCAACACGACCGAGTCCATTT
GGGCTTGCCTTAGCTGCTCCCATGTTGCCTGTGGAAGATATATTGAAGAGCATGCACTCA
AGCACTTTCAAGAAAGCAGTCATCCTGTTGCATTGGAGGTGAATGAGATGTACGTTTTTT
GTTACCTTTGTGATGATTATGTTCTGAATGATAACACAACTGGAGACCTGAAGTTACTAC
GACGTACATTAAGTGCCATCAAAAGTCAAAATTATCACTGCACAACTCGTAGTGGGAAGG
GTTTTTACGGTCCATGGGTACAGGTGATGATTCTTATTTCTTACATGACGGTGCCCAATC
TCTGCTTCAAAGTGAAGATCAACTGTATACTGCTCTTTGGCACAGGAGAAGGATACTAAT
GGGTAAAATCTTTCGAACATTGGTTTGAACAATCACCCATTTGGAAGAAAAAAGCAAGAA
GAACCATTTCAGGAANAAATAGTAGTAAAAGGAGAAGNTAAGAAAAGACGGCAGGAATTT
GGAGNTATCAAGTTAAAGCAGAATTGGNAAAGTATGCCTCAANAAAGAAANTTTACGTTT
ACAAGGGGCTCGCTCAGTCGACATATAGAAATNNTTTNTTGTTTGTTGAGGTGCCAGCAC
AAACGCCAGCATCACCACAAAGGATTAAGTACTCTCTACCTCAGAAATGAATATNTCAAA
AAAGTCGG
>OriGene 3' read for NM_032147 unedited
NTTTAGCTTTGGACCGGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTAATG
CTCAGGGCCAGTGTTTTAAGAGGCTCCTACCTAGTAGTCACATAATCCTAGCAAGAGTAA
ATCAAGCTTTAGTTATTTAGAATGAGATGCTAGACATAATAATAGTCCAGTTGCCAATTT
TGAACACTTATCTTTTCTGCCCTATAATTTTTTCTCTTCAAGACTCTATTCCTTTGAACT
AAAAAAAATACATAAAGTTTTAAGAGATCTAATGTCATCAGACATTATAAAATGAACTCA
TACCCAAGGTAAACTATTTGAATAACATCATACACTGGTTTACTAAATTCAGACTTTTTA
TGACTGCTTAATTATTAGACTTCTGGACAAACCTGTTTGCTGCCAGACAAAAATCATAAA
TCCCAGCCATCATTTTTTTCATGTCCATAGTTCCAGAGTTCACTTAAATATACTATTTGT
AAAGCTGCCATTTTCATTTATAGTTTGACTATGCCAAAGAGCAATTACTAAAGCAATTNT
TGGGGTTACATTAACCAAAATAATGTCAATTACAAGGCCTGTTAACACACAAAGCCTCAG
AAATACCAGTGAGTGTGACTGAAGATATTTAGGTGATACCTTGACATGTCACTTCAATAA
CTCCAAGGGNGCAAGGTAACTTCAATATACANATGAAAGACTATTAGTGACCAAAGTAGA
GTCTAATTTTTCATCTATTAAGAATTTGTATAGAAAATACCTGAAAAAGAACATGTATAT
AAAATGATGCCACTTGACTTTTGTTGGTAGAAGATTGCAACAGTTCATCATCCGAGCCCA
TTTAGACATANANTTTTANNAGAGGGTACCTCNGTCCTGAGGTAACACCCAAAGTTTAAA
ACTTNCAATATGAAAAAAGAAGTAACTCTCAGTGGATTTTACATTAAATACTTGTAAAAA
AAAAAATGTTGATATTAAAGGATAAAGTGGTATACCCG
Restriction Sites NotI-NotI     
ACCN NM_032147
Insert Size 3080 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032147.1, NP_115523.1
RefSeq Size 2871 bp
RefSeq ORF 2139 bp
Locus ID 84101
UniProt ID Q9H0E7
Domains UCH, zf-UBP
Protein Families Druggable Genome, Protease
Gene Summary The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome. [provided by RefSeq, Dec 2016]
Transcript Variant: This variant (1) encodes the same protein as variants 2, 3, 5, and 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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