Apc11 (ANAPC11) (BC000607) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	anaphase promoting complex subunit 11; anaphase promoting complex subunit 11 (yeast APC11 homolog); APC11; APC11 anaphase promoting complex subunit 11; APC11 anaphase promoting complex subunit 11 homolog; Apc11p; HSPC214; MGC882
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC000607, the custom clone sequence may differ by one or more nucleotides ATGAAGGTGAAGATTAAGTGCTGGAACGGCGTGGCCACTTGGCTCTGGGTGGCCAACGATGAGAACTGTG GCATCTGCAGGATGGCATTTAACGGATGCTGCCCTGACTGTCCTCTCCATGGAGAAAGCATTTCTAGGTG TTTGGGCTGGTGCCCGCAGCCTGTGCCTGTCCTGGGAGGCAGGGCCCATCCACAGGTGCCCATCAACACA GCTTCCCCAACGCCTGGGCAGCACACCGGATCCCTGATGTCTAGGGAAGAGTCTTCTAGGTCCCCAGACC CCACCCCTCCTGCCCTTGATCAAGAGACCAGTTCACTACTCAGATGCACGTCTCCTTGGTGCCTTGACCA TTCATGTGACCTTTTTGGCATCACAGATCAAGTGTCTGCAGATGGGCCCAGGGCCTGTAGGCAAGGTGCC CGGCGACGACTGCCCGCTGGTGTGGGGCCAGTGCTCCCACTGCTTCCACATGCATTGCATCCTCAAGTGG CTGCACGCACAGCAGGTGCAGCAGCACTGCCCCATGTGCCGCCAGGAATGGAAGTTCAAGGAGTGAGGCC CGACCTGGCTCTCGCTGGAGGGGCATCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC000607 unedited TTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGTCGGGCCGCGACTGTG GTCGTTTTTATACCTTCCCGCGCGGACGCCGGCGCTGCCAACGGAAGGGCGGGTAGGGCG GCTCTGCTGCCATGAAGGTGAAGATTAAGTGCTGGAACGGCGTGGCCACTTGGCTCTGGG TGGCCAACGATGAGAACTGTGGCATCTGCAGGATGGCATTTAACGGATGCTGCCCTGACT GCAAGGTGCCCGGCGACGACTGCCCGCTGGTGTGGGGCCAGTGCTCCCACTGCTTCCACA TGCATTGCATCCTCAAGTGGCTGCACGCACAGCAGGTGCAGCAGCACTGCCCCATGTGCC GCCAGGAATGGAAGTTCAAGGAGTGAGGCCCGACCTGGCTCTCGCTGGAGGGGCATCCTG AGACTCCTTCCTCATGCTGGCGCCGATGGCTGCTGGGGACAGCGCCCCTGAGCTGCAACA AGGTGGAAACAAGGGCTGGAGCTGCGTTTGTTTTGCCATCACTATGTTGACACTTTTATC CAATAAGTGAAAACTCATTAAACTACTCAAATCTTGAAAAANAAAAAAAAAAAACTCGAC TCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTG ACCCCTCCNCAGTGCCTCTCCTGGCCCTGNAAGTTGCCACTCCAGTGCCCACCAGCCTTG TCCTATAAAATTAAGTTGATCATTTTGTCTGACTAGGTGTCCTTCTATATATATGGGGAG GNNGGGGGGCGGTTGGAGACACAGGCGCAATTTGGGAAAAAACACTGTGGGCCTGGTGGG TTCATTGGGGAACAGCCGGCGTGGCGGGGACAATTTTGGCTTATTGCAACCTCCCTCCCT GGGTTCAGCGATTTCCTGCTCACCTCCGGAGTGTGGGATCN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC000607 unedited NNNCCGGTATTACTATGNNACCGCGGCCGCAANCTANGATCGGATTTTTTTTTTTTTTTT TTCAAGATTGAGTAGTTTAATGAGTTTTCACTTATTGGATAAAAGTGTCAACATAGTGAT GGCAAAACAAACGCAGCTCCAGCCCTTGTTTCCACCTTGTTGCAGCTCAGGGGCGCTGTC CCCAGCAGCCATCGGCGCCAGCATGAGGAAGGAGTCTCAGGATGCCCCTCCAGCGAGAGC CAGGTCGGGCCTCACTCCTTGAACTTCCATTCCTGGCGGCACATGGGGCAGTGCTGCTGC ACCTGCTGTGCGTGCAGCCACTTGAGGATGCAATGCATGTGGAAGCAGTGGGAGCACTGG CCCCACACCAGCGGGCAGTCGTCGCCGGGCACCTTGCAGTCAGGGCAGCATCCGTTAAAT GCCATCCTGCAGATGCCACAGTTCTCATCGTTGGCCACCCAGAGCCAAGTGGCCACGCCG TTCCAGCACTTAATCTTCACCTTCATGGCAGCAGAGCCGCCCTACCCGCCCTTCCGTTGG CAGCGCCGGCGTCCGCGCGGGAAGGTATAAAAACGACCACAGTCGCGGCCCGACCCTCGT GCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAAC GAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGG CGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCAT TGACGTCAATGGGGTGGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCA TTGGNTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTA
Restriction Sites	NotI-NotI
ACCN	BC000607
Insert Size	580 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC000607.1, AAH00607.2
RefSeq Size	901 bp
RefSeq ORF	591 bp
Locus ID	51529
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
Gene Summary	Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.