MTHFD1L (BC008629) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ292B18.2; DKFZp586G1517; FLJ21145; formyltetrahydrofolate synthetase domain containing 1; FTHFSDC1; methylenetetrahydrofolate dehydro; mitochondrial C1-tetrahydrofolate synthase; mitochondrial C1-tetrahydrofolate synthetase; MTC1THFS; OTTHUMP00000017418
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC008629, the custom clone sequence may differ by one or more nucleotides ATGGCGGTGCTGGCCCTGACGGACAGCCTCGCAGACATGAAGGCACGGCTGGGAAGGATGGTGGTGGCCA GTGACAAAAGCGGGCAGCCTGTGACAGCAGATGATTTGGGGGTGACAGGTGCTTTGACAGTTTTGATGAA AGATGCAATAAAACCAAACCTGATGCAGACCCTGGAAGGGACACCTGTGTTCGTGCATGCGGGCCCTTTT GCTAACATTGCTCACGGCAACTCTTCAGTGTTGGCTGATAAAATTGCCCTGAAACTGGTTGGTGAAGAAG GATTTGTAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCG AGCTTCCGGCTTGGTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGC GGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAGAAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAG ACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTTCCCGTTGTGGTGGCTCT GAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGCTGGTGCC TTTGATGCAGTCCCCTGCTATCACTGGTCGGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGA GAGAGGCTGCGAGTAAAAGAAGCCGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGAT AAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAACTCTCTCCTGAGGCACAAGCCAAAATA GATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACCCACCTTTCTCTATCTC ACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGG CGCTGGGTTCATTTACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTT TATGACATAGATCTTGATACCGAAACAGAACAAGTTAAAGGCTTGTTCTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008629 unedited NATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCGAGCACACTGA CAGAAGAGGAAGTGAGTAAATTTGCCCGTCTCGACATCGACCCATCTACCATCACGTGGC AGAGAGTATTGGATACAAATGACCGATTTCTACGAAAAATAACCATCGGGCAGGGAAACA CAGAGAAGGGCCATTACCGGCAGGCGCAGTTTGACATCGCAGTGGCCAGCGAGATCATGG CGGTGCTGGCCCTGACGGACAGCCTCGCAGACATGAAGGCACGGCTGGGAAGGATGGTGG TGGCCAGTGACAAAAGCGGGCAGCCTGTGACAGCAGATGATTTGGGGGTGACAGGTGCTT TGACAGTTTTGATGAAAGATGCAATAAAACCAAACCTGATGCAGACCCTGGAAGGGACAC CTGTGTTCGTGCATGCGGGCCCTTTTGCTAACATTGCTCACGGCAACTCTTCAGTGTTGG CTGATAAAATTGCCCTGAAACTGGTTGGTGAAGAAGGATTTGTAGTGACCGAAGCTGGCT TTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCTTGG TGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGANGCGGGC CAAGTGTAACGGCTGGGTGTCCTCTTAAGAAAGAATATACAGAGGAGAACATNCAGCTGG TGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTTC CNGTTGTGGGTGCTCTTGATGTCTTCAGACCGACACCCGCGCTGAAATGACTGGTGTGTG AGCTTGCAAAGCGGCTGGTGCTTTGATGCAGTCCCTGCTTCACTGGTCCGTGGGTGAAAG GGATCGTGAACTGGCTTGGCTGTANAAAGCTCCANTAAANAACCGATTCAGTCCTGATGA AGTCAGGTTCATTGGGGACAAAAAGACATGGTCAGCTGTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC008629 unedited NGGTTTAACTATGAACCCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTTTTT TGGTTTTCTAGATCCTGTTTATTTTCAGTTCAAAAACAAAGTAGAAAACTTGAGAGTGGA AAATGTCACCTTTTAGTTCACACTCTTAATCCCTTAGTCCCCATAAAATAAACATTCTAA AGTGTAAGCAGTAGAAATAATGGAAACTCCACAGAAACAGAAATAAATTAGTTTCTTTCA GTCTTGCTGGAGGTCCTTTTGCCGAACACCATACTCCACTGTGAACAGAATTCATCTTGA ACGAAGAAGAAATCTTTGGCCTATTTCACCATGTCTCCAGCATTGCATAACAGACATTTT TCAAATTCAGTTTCTTCTCCAACTGCAGCAAAAAGGCAAAGAGTAGTCTGTTTCAGGAGT CTGCATCGGGTCCTGTGAGAGCCTTGTCCACTTAGAACAAGCCTTTAACTTGTTCTGTTT CGGTATCAAGATCTATGTCATAAAAGCAGGGCCGGGTGGGCAGTCCTGGCATGGTGCTCA TCGTTCCGACCAAAGGGTAAATGAACCCAGCGCCTATGCTGGCCCGGACGTCACTGATAG GTAAGATGAAGTCCCTTGGCACACCTTTTTTGTCAGGTTGGTGAGATAGAGAAAGGTGGG GTCTTTGCCATGCAGATGGGCAAATTTCCAAAACCCTGTTGAGTGTAACGATCTATTTTG GCTTGTGCCTCAGGAGAAGTTCAATATCTTTGGCTCCATAGACAGCCTGAGCAATGGTCC TTATCTTGTCCACAATTGGAACCTGAACATCATACAAGGACTGGAATCGGGCTTCTTTTA CTCGCAGCCTCTCTCACAGCCCGAGGCAAGTTCACCGATTCTTTTCCACAACGGGACAGT GATAGCAGGGGAC
Restriction Sites	NotI-NotI
ACCN	BC008629
Insert Size	1850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008629.1, AAH08629.1
RefSeq Size	1759 bp
RefSeq ORF	1101 bp
Locus ID	25902
Domains	FTHFS
Protein Pathways	Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate
Gene Summary	The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.