MED6 (AF074723) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARC33; NY-REN-28
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF074723, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGGTGGATATCCGAGACAATCTGCTGGGAATTTCTTGGGTTGACAGCTCTTGGATCCCTATTT TGAACAGTGGTAGTGTCCTGGATTACTTTTCAGAAAGAAGTAATCCTTTTTATGACAGAACATGTAATAA TGAAGTGGTCAAAATGCAGAGGCTAACATTAGAACACTTGAATCAGATGGTTGGAATCGAGTACATCCTT TTGCATGCTCAAGAGCCCATTCTTTTCATCATTCGGAAGCAACAGCGGCAGTCCCCTGCCCAAGTTATCC CACTAGCTGATTACTATATCATTGCTGGAGTGATCTATCAGGCACCAGACTTGGGATCAGTTATAAACTC TAGAGTGCTTACTGCAGTGCATGGTATTCAGTCAGCTTTTGATGAAGCTATGTCATACTGTCGATATCAT CCTTCCAAAGGGTATTGGTGGCACTTCAAAGTACATGAAGAGCAAGATAAAGTCAGACCTAAAGCCAAAA GGAAAGAAGAACCAAGCTCTATTTTTCAGAGACAACGTGTGGATGCTTTACTTTTAGACCTCAGACAAAA ATTTCCACCCAAATTTGTGCAGCTAAAGCCTGGAGAAAAGCCTGTTCCAGTGGATCAAACAAAGAAAGAG GCAGAACCTATACCAGAAACTGTAAAACCTGAGGAGAAGGAGACCACAAAGAATGTACAACAGACAGTGA GTGCTAAAGGCCCCCCTGAAAAACGGATGAGACTTCAGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF074723 unedited ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTAAACGCTCTCG GAATTATGGCGGCGGTGGATATCCGAGACAATCTGCTGGGAATTTCTTGGGTTGACAGCT CTTGGATCCCTATTTTGAACAGTGGTAGTGTCCTGGATTACTTTTCAGAAAGAAGTAATC CTTTTTATGACAGAACATGTAATAATGAAGTGGTCAAAATGCAGAGGCTAACATTAGAAC ACTTGAATCAGATGGTTGGAATCGAGTACATCCTTTTGCATGCTCAAGAGCCCATTCTTT TCATCATTCGGAAGCAACAGCGGCAGTCCCCTGCCCAAGTTATCCCACTAGCTGATTACT ATATCATTGCTGGAGTGATCTATCAGGCACCAGACTTGGGATCAGTTATAAACTCTAGAG TGCTTACTGCAGTGCATGGTATTCAGTCAGCTTTTGATGAAGCTATGTCATACTGTCGAT ATCATCCTTCCAAAGGGTATTGGTGGCACTTCAAAGATCATGAAGAGCAAGATAAAGTCA GACCTAAAGCCAAAAGGAAAGAAGAACCAAGCTCTATTTTTCAGAGACAACGTGTGGATG CTTTACTTTTAGACCTCAGACAAAAATTTTCCACCCAAATTTGTGCAGCTAAAGCCTGGA GAAAAGCCTGTTCCAGTGGATCAAACAAAGAAAGAGGCAGATCCTATACCAGANACTGTA ANACCCTGAGGAGAAGGAGACCACCAAGATTGTACAACAGACAGTGAGTGCTAAAGGGCC CCCTGAAAACGGATGAGACTTACTCACTGAAAACAGCCCTCCCTGATGAGCCCCCATTGT ATGTATAGCCTAACCTTACCCCTGCTTCAGGGGTGCCAAGCCTGAAATCATTTGGCTTCT CAAACTGCCTGGG
Restriction Sites	NotI-NotI
ACCN	AF074723
Insert Size	3700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF074723.1, AAC26869.1
RefSeq Size	1007 bp
RefSeq ORF	741 bp
Locus ID	10001
Domains	MED6
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.