DDA1 (AL832922) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C19orf58; PCIA1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832922, the custom clone sequence may differ by one or more nucleotides GGCCATTGAGACTCGGTCTTGTCCCACGCTTCGCCCGGAACTTTCCCATGCCCAGGCCTCACTCAGCGTG CACGCACGTTGGGGAGAAGTCGGCCCTTGGGATCTTTCTCTTGAGTCATTTTATTTTTATCATGGACTAG TGCGTGCTCCGTGTCCACCCCAATAAAAGGGTCTTTCCTACTTGACCTCGCGTCTTTGCTCCACACACCT TGGCTGCAGAGGACGGAGCCCCTGGGAGTCATCCCTGTTGTCACCAAGAGGAGTGAGAAGGGCCCCCCAC GCCAGGGGCCCCAGGACCGGCTGGATTCTCTCGTGGGCTTGCTGAGGCCACCATGTTCAAGGCCAAGACC TGCCTGGACCATCCCCTCTGCCTCTCCTGTCAAGGTGACCCCAAGGACAGGTCATTCCTGAGAGACGGTC CATGGTGCCAAGGATGGAGCGGGGTCAGCCCCCTGCCTGGGATACCGCCAAGCAGGGCAGCGCACCTGTC AGACACCGCAGAGATGGCCGGCCTCATCCGGGGGCCTGGGGTCTCTGGCCTCTGCTGCTCCCCTGAGGCA TGGTGACAGTACAGACGGCATCTGGCCGGTCTTGCTGTCCTAGGTGGTGGCCTCACCCTTGCTGGGTCAT AGAGGAGCTGTACAGAAGGTTGGCACCTGGCAGTTGGCATGTGCCATGGCCGCTCATGGGGACCCCTCTT CCGCCAGCTGGGCTGGGTTTGGGGCTGAGCTTGGGTATGTAGGGGTGTTCGGGCTCTGGGCAGAAGGTTT GTGGCCCTGACCACTGAGCCTGGGGCTGGGGGAACACCGCTTCTCCCCTTATTGCCCCCACTGGTGGTGG TGGGGATCTCAGTTTTCTTTTGTTTTGTTTTGTTTGTTTGTTTGAGTTGGGGTCTTGTACTCTTTCCCAG GCTGGAGTGCAGTGGCGTAATCACAGCTCACTGCAGCCTCGACCTCAAGCCTTCCTCCCACCTCAGCCTT CCAAGCCGCTGGGACCACAGGCACCCACCACCATGCCTGGCTAATTTTTTTTTTTTTTTTGAGATGGAGT CTCGCTGTGTCTCCCAGGCTGGAGTGCAGTGGCAAGATCTCAGCTCACTACAACCTCCGCCTCCCAGGTT CAAGTGATTCTCCTACCTCAGCCTCCTGAGTAGCTGGGATTACACGCGTGCGCCCCTACACCCAGCTAAT TTTTCTATTTTTAGTGGAGACGGCGTTTTGTCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGT GCTTCGCCTGCCTCGGCCTCCCAAAGTGCTGTGACCGCACCTGGCTGCCTGGCTAATATTTTAAAAATTT TTTGTAGAGACAGGGTTTCACCATGTTGCCCAGGCTGGTCTCAAACTCCTGGGCTCAAGTCATCCACCTT CCTCAGCCTCCCAAAGTGCTGGGGTTACAGACGTGAACCACCGTGCCTGGCAGAGGTCTCTGTTTTGAGA GCTGAGTCTGAGGGCGTTGGGATGGTGTAGGGTTGGGCCACAGAGCTAGCAGACACTCCGGGCTATGGAG GGTCCGCCTGCCCCAGATTCCACTGTGGAGGGCCCTGCCTGTGTCAGACCCTCGGGCCTAGGCGGCCCAT CTCTCCCAAGGACGGAGAGTGCCAGGGTGGGCTCCCGTCCTACACAAGGAGTGGCTGACCAGGCCCAGGC ACAGCACAGCCACCCTGCTGTACCTCCCAGCTCCCGGCAGTGTCTACACCACAGTGATGGTGCTACAGGG GCCATGTGGTCACAGGACAGGTGGTCAGGAAATGCACACTTGACTGCGGTCGATGGTGCCGGGTCCGGCT GAATGCCCTGGAGGCTCCCAGGCCAGCTGGGGAGTCCCAGTCCCCAGGGTTGGTGTGACCTGGGCAGGCC TGCGGTGTCCATCTGTGAATGACTGAGATGGTGGGACTTCAGCTTCCAAGGCCAGGGGCCAGAGTGGGAG CAGGGGGCACTCCCGCCTTTCCCCTCGGCCTGTCTAGACCTCTCTGGCTCCCAGTTTCTGCTTTGAGTGG CTGGGGGCTGTCTCCTGCCTGTGCTGTCTGCCCGTCGCTCTGCAGGGCTGCTGGGCGGGCACCTCCTTCC CCTCCCCTACCTCAGCTCCCTCCTTTGGGGTCTGAGGGCTGTGCCCCCAGCTTGCCTCCCTGTGGCCTCC CCCTAGAACCCCTGGGTCTGTCGGGACATGCCCCACCTGGCACACACACCCTGTGTAGCTCCCACTGCCT GTGGGAGGACGCTGGGCCCCTGAGCCGGCCTCTGTCTACCCCTCCTGCCTTTGGGGTCCCCTTTACCCCA CTGTTCCCCCAAACTGCCTTCCACCTTCTCCGCCTTTGCACAGGCTGTTCTCCTGCCTCCAGTGAACTCT GACTTCTCCACAGCCTCCCAAAGGGCCCCTTGCTCACACTGGCCCAGCCCTGCAGCATGGCCATGTTGGT CTCTGAGCCTTAAGCCTTTGGATGTGGACACATTTGTCCTCTTCCTGAAAAATGTGAGGGTCTGAGAGGC CACGTTCCTGTTCCTGAGTCATACGACAGAGTCCCAGTGACTTGGGTCTGATGTCTGTCCCACAGTGGGA CATCCCTGCTGCATCCCAATGCCACCCCGTGTCCTGGGGACACCCAAATGTGCCATTCTCTCAGTATCAC AAGTCGGGGACTGCAGGAGGCTCAGGTGCCAGGTGCCTTCTGATGTTCATGCCACAAGCTCTTCCCACTG TCTCTGGCTCCCCAGACGATGTTCCAGGAGGCGGGTGTGCCAGAAGGGGCCACGTCTTGCAAACCACCGC GCTGTCCTCTTTGAGAAGGAGTCTTACTCAGGACTGGGGCCTGTGCACACATTGTCGCCTCTTTTCAGCA CTTAGAGATTCCTTCCTTTGTCTAGTGGCTGAAGCCAGGGCTGAAGTTGGCCTCCAAATCTGGGCCGTCT CAGAGGCGGCGCAGCCTGGAGTTTTCCATCTGTGGCCAAGACCCAGTTTTTGGGAGGAGGCCCTCATGGG TCAAGCCAGCCTGTACCAGGATGGGGGGAGGGGGTTCCCAACCTAGGCCCCAGCCACCCAGACTCCCCCA CCCCCACTCCCTTTTCCACTGCTCTGACCTCGGGCACTGTTGAAATATAGTTTTTATTGCATTTCTGCCG TTTTACAAAAATATGACAAAATAAATTAAAAACAAATAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832922 unedited ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGGCTGTTCTCCTGCCTCC AGTGAACTCTGACTTCTCCACAGCCTCCCAAAGGCCCCTTGCTCACACTGGCCCAGCCCT GCAGCATGGCCATGTTGGTCTCTGAGCCTTAAGCCTTTGGATGTGGACACATTTGTCCTC TTCCTGAAAAATGTGAGGGTCTGAGAGGCCACGTTCCTGTTCCTGAGTCATACGACAGAG TCCCAGTGACTTGGGTCTGATGTCTGTCCCACAGTGGGACATCCCTGCTGCATCCCAATG CCACCCCGTGTCCTGGGGACACCCAAATGTGCCATTCTCTCAGTATCACAAGTCGGGGAC TGCAGGAGGCTCAGGTGCCAGGTGCCTTCTGATGTTCATGCCACAAGCTCTTCCCACTGT CTCTGGCTCCCCAGACGATGTTCCAGGAGGCGGGTGTGCCAGAAGGGGCCACGTCTTGCA AACCACCGCGCTGTCCTCTTTGAGAAGGAGTCTTACTCANGACTGNGGCCTGTGCACACA TTGTCGCCTCTTTTCAGCACTTAGAGATTCCTTCCTTTGTCTAGTGGCTGAAGCCAGGGC TGAAGTTGGCCTCCAAATCTGGGCCGTCTCAGAAGCGGCGCAGCCTGGAGTTTTCCATCT GTGGCCAAGACCCAGTTTTTGGGAGGAGGCCCTCATGGGTCAAGCCAGCCTGTACCAGAT GGNGAAGGGGGGGGGTCCCAACCTAAGCCCCCAGCCACCAGACTCCCCCACCCCCATCCC CTTTTCACTGCTCTGACCCTCGGCACTGGTGAAAAATAATTTTTATTGCATTTCTGCCGG TTTTCAAAAATTGGCCANATTAATTTTAAACCAATTAAAAAAAAAAAAAAACCTCGCTCT AGATTGCGGCCGCGTCTTAGCTGTTCTCTGAACGACC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL832922 unedited AAACCGTATNACCGCCCCCGCTANCTATGATCGAGTTTTTTTTTTTTTTTTTTATTTGTT TTTAATTTATTTTGTCATATTTTTGTAAAACGGCAGAAATGCAATAAAAACTATATTTCA ACAGTGCCCGAGGTCAGAGCAGTGGAAAAGGGAGTGGGGGTGGGGGAGTCTGGGTGGCTG GGGCCTAGGTTGGGAACCCCCTCCCCCCATTCTTTTACAGGCTGGCTTGACCCATGAGGG CCTCCTCCCAAAAACTGGGTCTTGGCCACAGATGGAAAACTCCAGGCTGCGCCGCCTCTG AGACGGCCCAGATTTGGAGGCCAACTTCAGCCCTGGCTTCAGCCACTAGACAAAGGAAGG AATCTCTAAGTGCTGAAAAGAGGCGACAATGTGTGCACAGGCCCCAGTCCTGAGTAAGAC TCCTTCTCAAAGAGGACAGCGCGGTGGTTTGCAAGACGTGGCCCCTTCTGGCACACCCGC CTCCTGGAACATCGTCTGGGGAGCCAGAGACAGTGGGAAGAGCTTGTGGCATGAACATCA TAAAGCACCTGGCACCTGAGCCTCCTGCAGTCCCCGACTTGGGATACTGGAATAATGGCC CATTTGGGGTGTCCCCCAGACACGTGGTGGCCTTTGCGATGCCCCAGGGATGTCCCCACT GTGGGAACAGAGATTTTGAATCACAATCCACTGGGGCCTCTGGCCCCTAGGACTCCGGGA ACCAGGACCTTGTCCTTCTCCAACCTTTCATTTTTTCAGCAAAAAGCGCCACTGTTTTCT CCATCTCCCGGGCTTTAGGGACGAAGGTACCTTACCTGCCCTTGCTGTTAGGCTTGGGCC CCGTGCCGACCACGGNGCCCTTTGGGCAGGCTCGCGGGGAGCACCGCACCTACACCGGGG GCTGGAAAACAGCGCGTCTGGCGCCGGAACTCCCGCGCCCCCCGCATCCGG
Restriction Sites	NotI-NotI
ACCN	AL832922
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832922.1
RefSeq Size	3202 bp
RefSeq ORF	3202 bp
Locus ID	79016
Gene Summary	May be involved in ubiquitination and subsequent proteasomal degradation of target proteins. Component of the DDD-E2 complexes which may provide a platform for interaction with CUL4A and WD repeat proteins.[UniProtKB/Swiss-Prot Function]

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