Glutaredoxin 1 (GLRX) (BC010965) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	glutaredoxin (thioltransferase); GRX; GRX1; MGC117407; OTTHUMP00000149979
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105918 sequence for BC010965 edited (data generated by NextGen Sequencing) ATGGCTCAAGAGTTTGTGAACTGCAAAATCCAGCCTGGGAAGGTGGTTGTGTTCATCAAG CCCACCTGCCCGTACTGCAGGAGGGCCCAAGAGATCCTCAGTCAATTGCCCATCAAACAA GGGCTTCTGGAATTTGTCGATATCACAGCCACCAACCACACTAACGAGATTCAAGATTAT TTGCAACAGCTCACGGGAGCAAGAACGGTGCCTCGAGTCTTTATCGGTAAAGATTGTATA GGCGGATGCAGTGATCTAGTCTCTTTGCAACAGAGTGGGGAACTGCTGACGCGGCTAAAG CAGATTGGAGCTCTGCAGTAA Clone variation with respect to BC010965.1 225 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC010965 unedited ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCTCGTGCCGAATTCGGCACGAG GGCAATACCTGCAACTGAGGATTCTTCCCGGGGAGACCGCAGCCCATCGGCATGGCTCAA GAGTTTGTGAACTGCAAAATCCAGCCTGGGAAGGTGGTTGTGTTCATCAAGCCCACCTGC CCGTACTGCAGGAGGGCCCAAGAGATCCTCAGTCAATTGCCCATCAAACAAGGGCTTCTG GAATTTGTCGATATCACAGCCACCAACCACACTAACGAGATTCAAGATTATTTGCAACAG CTCACGGGAGCAAGAACGGTGCCTCGAGTCTTTATCGGTAAAGATTGTATAGGCGGATGC AGTGATCTAGTCTCTTTGCAACAGAGTGGGGAACTGCTGACGCGGCTAAAGCAGATTGGA GCTCTGCAGTAACCACAGATCTCATAGGAAATGTTCAACAATTCTGTGAAAGGTCACAGG ACCCAATTGGAGAAATCATATGAAAAGCATAGTTGGTCTTGGTGTCATATGGATCAGAGG CANCAGTGCAGAGGCTGTGGTCATGCGGAACACTCTGTTATTTAAGATGGCTATCCAGAT AATCCTGAACACTGTGTATTTATTTTATTTAGACTACCAGCAAAGATTAAAGCATGAAAT GTAAAACATCTGATAAAACTTACAGCCCCCTACACCAAAGTGTATCTGTGAAAAGCTCCT ACACTTTGAAAACTAAGAATCCCTTACATGAAGTTGCCTGTTCTANAATTGAAGAATGTA ATTTCCCTCATCTCTAGGACACACTTAATTTCTTTTCTATAAAAAAACCCTTTGATGAAA AAAAAAAAAAAACTCTATTTATATGGCGGCCGCGTCATAGTTGTTTCTGAAAAAACCCGG TTGGGATCCTGTGACCCCTCCCATGCTTTTCTGCCCTGAAATGCCCCTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC010965 unedited GGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATCTATAGGTTTTTT TTATTAAAAAAAAAATTAAGTGTTGTCACTAGAGATTGAAGGAAATTAACAATCTTACAA TTCTAGAACAGGCAAACTTCATGATAAGGGATTCTTAAGTTTTCAAAGTGTAGGAGCTCT TTCACAGATACACTCTTGGTGTAGGGGGCTGTAAGTTTTATCAGATGTTTTACATTTCAT GCTTTAATCTTTGCTGGTAGTCTAAATAAAATAAATACACAGTGTTCAGGATTATCTGGA TAGCCATCTTAAATAACAGAGTGTTCCGCATGACCACAGCCTCTGCACTTGTGCCTCTGA TCCATATGACACCAAGACCAACTATGCTTTTCATATGATTTCTCCAATTGGGTCCTGTGA CCTTTCACAGAATTGTTGAACATTTCCTATGAGATCTGTGGTTACTGCAGAGCTCCAATC TGCTTTAGCCGCGTCAGCAGTTCCCCACTCTGTTGCAAAGAGACTAGATCACTGCATCCG CCTATACAATCTTTACCGATAAAGACTCGAGGCACCGTTCTTGCTCCCGTGAGCTGTTGC AAATAATCTTGAATCTCGTTAGTGTGGTTGGTGGCTGTGATATCGACAAATTCCAGAAGC CCTTGTTTGATGGGCAATTGACTGAGGATCTCTTGGGCCCTCCTGCAGTACGGGCAGGTG GGCTTGATGAACACAACCACCTTCCCAGGCTGGATTNTGCAGTTCACAAACTCTTGAGCC ATGCCGATGGGCTGCNNGTCTCCCCGGGAAGAATCCTCAGTTGCAGGTATTGCCCTCGTG CCGAAATCGCACGAGGCTCGTGCCGAAATCCCGGCCGCCCTATAGTGAGTCGTATTACAA AATCTGACGGTTACTTAACGAGCCTGCTTATTAGACCTCA
Restriction Sites	NotI-NotI
ACCN	BC010965
Insert Size	850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC010965.1, AAH10965.1
RefSeq Size	836 bp
RefSeq ORF	321 bp
Locus ID	2745
Domains	glutaredoxin
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2011]

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