PDSS2 (AF086233) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA59I9.3; C6orf210; COQ10D3; DLP1; hDLP1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF086233, the custom clone sequence may differ by one or more nucleotides GTGGTGCTCCTTATCTCTAAAGCAGCTGGGCCCAGCAGCGTGAACACTTCATGTCAGAACTATGACATGG TCAGTGGGATCTACTCATGTCAAAGAAGTTTGGCAGAGATCACGGAGCTAATTCATATTGCTCTCCTTGT ACATCGTGGGATAGTAAATTTAAATGAGTTGCAATCATCTGATGGTCCACTGAAAGACATGCAATTTGGA AATAAAATTGCTATCCTGAGTGGAGACTTTCTTCTAGCAAATGCCTGCAATGGACTAGCTCTGCTACAGA ACACCAAGTCATTTTCATTTAATGGACCTATTGCTATATATCAGATGGGAGACTGTGAGTCAGCCTGGAT CTTGTCTAAACACCCAAGAGCATTGTCATGATGTCACTCCTGGAGGTTGTTCTATTAAGCATTAGTGATT TATACTTCCGGATAACCAGTGGCACAGATGTACTCTTTACATAGCTGATGTATTTCCTTATGTTAACTTT GTTTTCCTAAAGGCGTAAATATGTTGTTTAAAATGAAGTATTTTGTTTGTACAAATATGCTCAATTTATG CATATAAATCTCCCTTTACACCATCTATATAGTTTAATAAAGATGCTATTGTCTAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF086233 unedited ACTCCTATAGGCGGCCGCGACTTCGGCACGAGGGCCTGCTGAGCGACGAGCTCAGCAACA TCGCTATGCAGGTGCGGAAGCTGGTGGGCACTCAGCACCCTCTGCTTACCACAGCCAGGG GGCTTGTACATGACAGCTGGAATAGCCTCCAGTTGAGGGGCTTGGTGGTGCTCCTTATCT CTAAAGCAGCTGGGCCCAGCAGCGTGAACACTTCATGTCAGAACTATGACATGGTCAGTG GGATCTACTCATGTCAAAGAAGTTTGGCAGAGATCACGGAGCTAATTCATATTGCTCTCC TTGTACATCGTGGGATAGTAAATTTAAATGAGTTGCAATCATCTGATGGTCCACTGAAAG ACATGCAATTTGGAAATAAAATTGCTATCCTGAGTGGAGACTTTCTTCTAGCAAATGCCT GCAATGGACTAGCTCTGCTACAGAACACCAAGGTTGTGGAACTTTTAGCAAGTGCTCTTA TGGACTTGGTACAAGGAGTATATCATGAAAATTCTACTTCAAAGGCTCAAGAAAAGGAAG ATTGGACTATGCTAAGTTGCGAGAAAAATCAAAGCTGGCANAGGTGTGACTTCACTATTG ACCTGGTGTCGTTACCATGGAAACAAGCACTGGAGCCTGNAAGCTTCCTCCTTCGAGCCC AGATCTGTTAAAAATTTGGNTTTGCTGGACCGATTTCATGACATCATTAAAGAACTTTTG TTATTACTGAATCTAGATNAAAGGTTTNTGGGAACCTTACCAGCGTAATGACTTAAAATG CATTTTCTCTATAGNANANAAAAAAAGTTTTTTTTTTTTGGACTGACACAATAAAAAGGG GGGGGGGTTTTCCTGCCGCGACACTGGGGTGGGGGCGAAAATAAGGAAAACTTCTGACTC CAGAGGAATTCTTTTTTTTGCCCAGGCCCNCGGNNCCCCCNTTTTTTTTTTTTAGAAAAA AAAAACTGGGAAAACTACAA
Restriction Sites	NotI-NotI
ACCN	AF086233
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF086233.1
RefSeq Size	624 bp
RefSeq ORF	624 bp
Locus ID	57107
Protein Pathways	Terpenoid backbone biosynthesis
Gene Summary	The protein encoded by this gene is an enzyme that synthesizes the prenyl side-chain of coenzyme Q, or ubiquinone, one of the key elements in the respiratory chain. The gene product catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains, the first step in coenzyme Q biosynthesis. Defects in this gene are a cause of coenzyme Q10 deficiency.[provided by RefSeq, Oct 2009]

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