GRF2 (RAPGEF1) (BD140654) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD140654, the custom clone sequence may differ by one or more nucleotides GCCCTTTACCCCCAACCCCAGGCCACTGGGCCCTTCCCACACCACTTGGGGAGCTGAGAAGAGGAGGCTG GAGTAAGGGAGGACTTGATCATCCAAGAAATACTTTTTATTGCTGGGAGTCTTCTGAACCTCACCAAACT GAGGCCAGAGCTGAGCTCCTGGGGGAGTTAATTCAGAGGGGAGAGGCCAGCACCTCCCTCCTCCATYGCT CGCTGTGTGCCTTAAACTCCATCTCATGTCCCTCCCCATCCCCTGGCTTTCCCTCCCTCCTTGCCCCATC CTGGGCCAGCCAGCAGGGCTCCTCCTCTGGCTCTTCAGACCTTTCAGCCAGTGCTGTCAGTGCCCCTGGG AGGGAAGGGCATCCCTGAGGCACCCGAATGGTCCCTCAGGGTGCAGGGAGGCAGAAGCCTGGCCACAGAG GAGCCTCCTAAGGCAGCAGCTGCAGCAAGCGCACCCTCTCCCCACTCTCCCCACGCCAGAGCGGCTTCCA GAGCAGATGCTGTTTCCATCCTCCTCGTCAAAACCATTCTCGCTGCTGAGCTTGACAATCTGGGCAAGGC TTGTGGGGCGCTTGACAAACAGAATCTGCCCTGTGCCGCCTGGTTCCGTGGCCTCCAGCATGAGCCTGCA GGCAGGGCGCTGCGGGAACCCAGTTGTGCTGCCCCAGCCCATGCCTCCGGGTCTGCTGTGCATGAATGAG TGCTCACTTGTCCCGGGTTTAGGACGTGGTCAAGTGAACAGCAGGGTCTAACTGTGCTTACTTAGCCCAG TTCAAACAGAACAAAGGAAAAATATAGAAAGCAACATCTGTTGATCATTTAGGTTTTTTTTTAAACCACC ATGTCACTTTGAGTCCTTCATGGGTTTTTGAACAGCATTTATCAAGAAGAAAATGTGGGCTTTTTCCCCT CTCCCGTGTTTTGTTTGTCCTGTAGATAGAGGGAGGAAAGCCGTGCAGTGGCAGGCGGGACCCCCTCTGG TGGCGGGACCCCCTCTTGCGGTGGTCTTGCGGGGCCAGCCGGGACCTGTCACTTTATTATTTAAGGAGTG TGTGTGTAGAGTCGCTGGCTTATTAACAGTATTGTGTGTGGGTTGGGTTTTTAGTTTGTTCCTTCTTTTT GAAGTCCCTTCATTTCAATCCTTGACTCTCTCTCCCCTTCCCTTGCCCAGCTCTGTTGAATGCTGCTGTG CGCGTGTGAGGGCCGCTCTGCACACAGGGCCCTTGGGTTGTGTGAACTGAAATTCTCCCTGTATTTGTGA GACTCGCAGGAGTCCCCATCTGTAGCACAGGCAATGCCAGTGCCATGCTGCAGCCTCAGAAACCAGGCCT CTCACTCCAGCAGCAGGCAGAACCGTGTCTGTGGTCGGGTGCTGTCCACAGCTCTGTCTGCCTTGTTCTT GGGCTTGAGCTGGATAGAGGTGGGGTCTCTTCACCTTCCCTGAATTCAGAACAGACCCTGTGCCTGGCCC CAGTGTGCCCAGGCAATTCCCCAGGCCCTCATTGGGAGCCCTTGGTGTTCTGAGCAGCAGGGCCCAGGCA GCACATGAGCAGTGCCCAGGGGCTCCCTGCGTGAGGACGGCAAGGTGCGATGTATGTCTAACTTATTGAT GGCAGGCAGCCCCCTGTGCCCCCTAAGCCTGGCCCTGGTTATTGCTGAGCTCTGTGCTCAGTGCTGCGGC CTGGCCGTGGCTCGTCTGTTCCTTTGGGGGGCCCGGGCGGGTTGTGGGAATCAGTCTTCACAGACAGACG TGAGCCAGGCGGAGGACTCGTTCCTTGCAGAGGTCAGTCCTCACCTGCAGGTGTCGGGGTGGRGGGGGGC AAGGAGGGGCAGGCACACACCATGTCTGACCTGAACCCGATTCTGGGGAGCATCTTCCCGCTCCGGCCCC ACGACCTCCACAGGGTTACATTGTAATATATATGCCCCAGCTAACCTGTCTGATGGTGGCATCTTCCTGC AGACATTTCAAACATGTAACTTTTATATGAAAAAAAATAAACACAGATGAAAGCTGCCCAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD140654 unedited GGTGCAGCATTTGTAACCGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCAGCACCT CCCTCCTCCATCGCTCGCTGTGTGCCTTAAACTCCATCTCATGTCCCTCCCCATCCCCTG GCTTTCCCTCCCTCCTTGCCCCATCCTGGGCCAGCCAGCAGGGCTCCTCCTCTGGCTCTT CAGACCTTTCAGCCAGTGCTGTCAGTGCCCCTGGGAGGGAAGGGCATCCCTGAGGCACCC GAATGGTCCCTCAGGGTGCAGGGAGGCAGAAGCCTGGCCACAGAGGAGCCTCCTAAGGCA GCAGCTGCAGCAAGCGCACCCTCTCCCCACTCTCCCCACGCCAGAGCGGCTTCCAGAGCA GATGCTGTTTCCATCCTCCTCGTCAAAACCATTCTCGCTGCTGAGCTTGACAATCTGGGC AAGGCTTGTGGGGCGCTTGACAAACAGAATCTGCCCTGTGCCGCCTGGTTCCGTGGCCTC CAGCATGAGCCTGCAGGCAGGGCGCTGCGGGAACCCAGTTGTGCTGCCCCAGCCCATGCC TCCGGGTCTGCTGTGCATGAATGAGTGCTCACTTGTCCCGGGTTTAGGACGTGGTCAAGT GAACAGCAGGGTCTAACTGTGCTTACTTAGCCCAGTTCANACAGAACANAGGANAATATA GAAAGCAACATCTGTTGATCATTTAGGTTTTTTTTTAAACCACCATGTCACTTTGAGTCC TTCATGGGTTTTTGAACAGCATTTATCAAGAAGAAAAATGTGGGCTTTTTTCCCTCTCCC GTGTTTTGTTTGTCCTGGTANATAGAGGAAGGAAGGCGTTGCCATGGCAGGGCGGGACCC CCTCTGTGGCGGGCACCCCTCTTGGCGGGGTCTTGCGGGGCCGCCCGGACCTGTCCCTTA TTTTTAAGGATGTGGGGTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BD140654 unedited NTTAGGTACACTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTGG GCAGCTTTCATCTGTGTTTATTTTTTTTCATATAAAAGTTACATGTTCTGAAATGTCTGC AGGAAGATGCCACCATCAGACAGGTTAGCTGGGGCATATATATTACAATGTAACCCTGTG GAGGTCGTGGGGCCGGAGCGGGAAGATGCTCCCCAGAATCGGGTTCAGGTCAGACATGGT GTGTGCCTGCCCCTCCTTGCCCCCCCCCACCCCGACACCTGCAGGTGAGGACTGACCTCT GCAAGGAACGAGTCCTCCGCCTGGCTCACGTCTGTCTGTGAAGACTGATTCCCACAACCC GCCCGGGCCCCCCAAAGGAACAGACGAGCCACGGCCAGGCCGCAGCACTGAGCACAGAGC TCAGCAATAACCAGGGCCAGGCTTATGGGGCACAGGGGGCTGCCTGCCATCAATAAGTTA GACATACATCGCACCTTGCCGTCCTCACGCAGGGAGCCCCTGGGCACTGCTCATGTGCTG CCTGGGCCCTGCTGCTCAGAACACCAAGGGGCTCCCAATGATGGCCTGGGGAATTGCCTG GGCACACTGGGGGCCAGCACAGGGTCTGTTCTGAATTCAGGGAAGGTGAAGAGACCCCAC CTCTATCCAGCTCAAGCCCAAGAACAAGGCAGACAGAGCTGTGGACAGCACCCGACCACA GACACGGTTCTGCCTGCTGCTGGAGTGAGAAGCCTGGGTTTCTGAGCTGCAGCATGGCAC TGGCATTGCCTGTGCTACAGATGGGGACTCCTGCGAGTCTCACAAATACAGGAGAATTTC AGTTCACACAACCCAAGGGCCCTGTGTGCAAC
Restriction Sites	NotI-NotI
ACCN	BD140654
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BD140654.1
RefSeq Size	2083 bp
RefSeq ORF	2083 bp
Locus ID	2889
Protein Families	Druggable Genome
Protein Pathways	Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
Gene Summary	This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

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