STK3 (BD127594) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD127594, the custom clone sequence may differ by one or more nucleotides AAAAGTTGGTCCGAGTTCCCAGAGTTTCCCTCTGTGGTGCCCTAGGCTCGGCCGGCCGGTGCCCCGGCTC CTTTCCTCCTTTCGGCCTTCGCCGTCCACCAGGTCCCTCTCTCTGTCCCCGGCCGCCATGGAGCAGCCGC CGGCGCCTAAGAGTAAACTAAAAAAGCTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGTTTTTGA TGTATTAGAGAAGCTTGGAGAAGGGTCTTATGGAAGTGTATTTAAAGCAATACACAAGGAATCCGGTCAA GTTGTCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGGAAATAATCAAAGAAATTTCCATAATGC AGCAATGTGACAGCCCATATGTTGTAAAGTACTATGGCAGTTATTTTAAGAATACAGACCTCTGGATTGT TATGGAGTACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGATTACGAAACAAGACAGGATTCTTAACA GATACCACTGAAGGAAGTGCTAGCTGTGATTTGAGAATGGATCTAACGCTTTTCCATCTTGGAGCTGAAT TCTTACATTACCTTCTTCTATATGAAGCAGATCCTCTCTCATGTGTTTTCTCCCTCTTGGTGTTTTCAGT ATAAATCTGACCATTTTGAAATAGTTGGGTATGGACCTATCCAGTCTCATGAAAAGTCTTACAGATATGA GAAAGAATTAAGCATCTGTTTTCAATAAAATGCTCATAAAGCCACCCATTAATGTACTCAGGGTGAAAGA AATCAAAAAAATTATTGGCCAGTTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGA CTAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAG AAGGACATGCAAAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATAC TGTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTGATTCAAGAAATAGGCTATAACTGTGTGGCCGAC ATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAACCTCCTTATGCTGATATACATCCAA TGAGGTAATGCCTTGATTGGTTGTGTCACCTGTGCAGTAGTTGAAAATGGATATGAGTACACAATTTCAG GACCTCCCTGCTTCCTTGTAGATCATTTGGCCGTATGCTATTTTTATGATTCCCACAAATCCACCACCAA CATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACCGATTTTGTTAAAAAGTGTTTGGTGAAGAATCC TGAGCAGAGAGCTACTGCAACACAACTTTTACAGGTCAGTGCTTGTGCCTCGAAGGGAAGGATATTTTCT TCATACCTTTCAGCTTTCTGACATTGGCCAGAATTGCTGTGTGTTTCATAACTGATTTCTAAAGATGTAA ACCTCCCCTCCCCTCCTGTTTGCTCCCTTCTTTTCTTTCCTCCCCTCTCGTCCCCTCATTTCCCCCAGAG TCATTTTAAAACAAAATTTCCAAAGCATTCTCCTTTCAGAAAGGAATCTTTGTCTTCATAGTTACTATTT CTCATGTTTTTTTTTTCTTCTGTTCCTTGACTCAGCATTTATCCTTTGACTTTTTGCTCCACCATAAAAT ATGTAAGTACAAGATCCCTGGACTCCTTGAAGAGATTATCACGTTGTAAATATCAGTTACAGGATTATCT AAATGAAGAGCTTCAGAGTTTCAATTTTTTTTGATAGAATAATTGTAAGTAGACTTTGTATCAGAATGTC AGTAGATTTTAAAATCTACCTTAAAATTTAATGCAAATAGAAATAATTTAATATTTATAATAGGTGTTTG TGAATCTTAATTTCCCCAACAGAATAGTCTTAGAACTTTCAGCTAATAATAAGTATGTCTCACATTAACA AAACTTGATATTTGCTAAATGATTTCTATTGTTAAGTGACAAAAGGATGAAAAACATGGTTCTTGTAAAA AGGGACTTCCCTGTCTCTAAAGGAACCATTGGGTT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD127594 unedited TTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCCTCTGTGGTGCCCTA GGCTCGGCCGGCCGGTGCCCCGGCTCCTTTCCTCCTTTCGGCCTTCGCCGTCCACCAGGT CCCTCTCTCTGTCCCCGGCCGCCATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAAA AGCTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAGC TTGGAGAAGGGTCTTATGGAAGTGTATTTAAAGCAATACACAAGGAATCCGGTCAAGTTG TCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGGAAATAATCAAAGAAATTTCCA TAATGCAGCAATGTGACAGCCCATATGTTGTAAAGTACTATGGCAGTTATTTTAAGAATA CAGACCTCTGGATTGTTATGGAGTACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGAT TACGAAACAAGACAGGATTCTTAACAGATACCACTGAAGGAAGTGCTAGCTGTGATTTGA GAATGGATCTAACGCTTTTCCATCTTGGAGCTGAATTCTTACATTACCTTCTTCTATATG AAGCAGATCCTCTCTCATGTGTTTTCTCCCTCTTGGTGTTTTCAGTATAAATCTGACCAT TTTGAAATAGTTGGGTATGGACCTATCCAGCCTCATGAAAAGTCTTACAGATATGAGAAA GCAGAATTAAGCATCTGTTTTCCAATAAATGCTCATAAAGCCACCCATTAATGTACTCAG GGTGGAAAGGAATCAAAAATTATTGGCCAGTTAATAGAAGATGAAATTGCCACCATTCTT TAATTCTACATTGNAAAGGACTCAGATAATTTGCCCTTTTATGAGGAAAACTACCCCGGA GATTTAAAAGCTGGGAATTT
Restriction Sites	NotI-NotI
ACCN	BD127594
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BD127594.1
RefSeq Size	2065 bp
RefSeq ORF	2065 bp
Locus ID	6788
Domains	TyrKc, S_TKc
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	MAPK signaling pathway
Gene Summary	This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

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