SIAH1 (AK094663) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SIAH1A
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105440 sequence for AK094663 edited (data generated by NextGen Sequencing) ATGGTTATAATTATTTTTCTCCTGCCTCCTTATGTATTTATTTCAGAAATGAGCCGTCAG ACTGCTACAGCATTACCTACCGGTACCTCGAAGTGTCCACCATCCCAGAGGGTGCCTGCC CTGACTGGCACAACTGCATCCAACAATGACTTGGCGAGTCTTTTTGAGTGTCCAGTCTGC TTTGACTATGTGTTACCGCCCATTCTTCAATGTCAGAGTGGCCATCTTGTTTGTAGCAAC TGTCGCCCAAAGCTCACATGTTGTCCAACTTGCCGGGGCCCTTTGGGATCCATTCGCAAC TTGGCTATGGAGAAAGTGGCTAATTCAGTACTTTTCCCCTGTAAATATGCGTCTTCTGGA TGTGAAATAACTCTGCCACACACAGAAAAAGCAGACCATGAAGAGCTCTGTGAGTTTAGG CCTTATTCCTGTCCGTGCCCTGGTGCTTCCTGTAAATGGCAAGGCTCTCTGGATGCTGTA ATGCCCCATCTGATGCATCAGCATAAGTCCATTACAACCCTACAGGGAGAGGATATAGTT TTTCTTGCTACAGACATTAATCTTCCTGGTGCTGTTGACTGGGTGATGATGCAGTCCTGT TTTGGCTTTCACTTCATGTTAGTCTTAGAGAAACAGGAAAAATACGATGGTCACCAGCAG TTCTTCGCAATCGTACAGCTGATAGGAACACGCAAGCAAGCTGAAAATTTTGCTTACCGA CTTGAGCTAAATGGTCATAGGCGACGATTGACTTGGGAAGCGACTCCTCGATCTATTCAT GAAGGAATTGCAACAGCCATTATGAATAGCGACTGTCTAGTCTTTGNCACCAGCATTGCA CAGCTTTTTGCAGAAAATGGCAATTTAGGCATCAATGTAACTATTTCCATGTGTTGA Clone variation with respect to AK094663.1 827 c=>n 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094663 unedited GTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGATTCTCTTGTATAATTTAAG TGATCTCTGGATTACTTAATACAATGTAAACAATATGTAAATAGTTGTTATAGACTGTAT TTTAAAAATTTTGTATTCTTTATAAATTTTTCTGAATATTTTCAATCCATGGCTGGTGAA GTCCTCGGATGCAGACCGTGTGGATACAGAGTGCCGATTTTATACAGGAGTTCACCTGTA ACTCCCTGTACCTATCAACAGCTGACTCCAAATTAGAAAGAAATAGAGTAAGGGAGCCTC AGGGAGAGTCCTAGCAAAACGGATTCGATTAAACTTCAGTTCCTTGTATAGTTTCTTTAG TTGTTTATGGTCCATTTTCTATTTTAGCATTTATTATTCTATGTAGTCTATCCAAAGACG ATTAAGGGAGTTCCACATGTTTTCCGGAACATTTTGAAAAGAGAGCTTATCCAGTGTACA GATCCTAATAAAGTGCACATTCAGTGTAATTTTATTTTTTTAATATCTTTTTTAATCCTA TTTTTCTTCCTCTTTTGCTCAGTAAATTTTGTATGAAACTTTAAAAGGACTTATGGCATG TAAACATTATTTATAAAGTAAGTCATGGTTATAATTATTTTTCTCCTGCCTCCTTATGTA TTTATTTCAGAAATGAGCCGTCAGACTGCTACAGCATTACCTACCGGTACCTCGAAGTGT CCACCATTCCCAGAGGTGCCTGCCCTGACTGGCACAACTGCATCCAACANTGACTTGGCG AGTCTTTTTGAGTGTCCAGTCTGCTTTGACTATGTNGTACCGNCCATTCTTCATGTCAGA GTGCCATCTTGTTTGTAGCAACTGTCGCCCAAGCTCACATGTNGTCCAACTGCCNGGGCC NNTTTGGATCCANCGCACTTTGCTTNGAGAAAGTGCTN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094663 unedited CTATGTACTCGGCCGCAATCGAGAGTCGAGTTTTTTCTTTTTTTTTTTTTAATTTGCATT TTTTTTTTTTAATGTATAATTATGAAAGACCAAAAACATTTTTTGCAAACTGCCTTTTTA AACAAATGATTTGCTTTTAATTACAAATACTGTGCATGACGATGCCTTCTTCTGCAAAAC CAATAAATACAAGAACAAATTTTAAAATATGTGATTTGTCCAAGATATCTGAAAAATGAA ACAAAAGCCTGATTTGCAGTATTTATAAAAATCAATTTATTCTCTAACCTTTCAAAGATT AAGCTCAAAGGTGTGACTCATCTGTAGAAGACAGAAAAGAGAGCACCTTATAGATGCTTT AAATAGCTTTTGATTATTTTTCCGATGTCACCTTTACTTTCTGGAACTCATGGCTTCACA AAATAGCTGATTTTAAAATATTTCTAATGAACTGATTAATGGGGGAAAAGAAAATATTGA ACACGTTATAAATTTTTTACCATAAACAAATATGCATATCAAAAGATACTAACTTTAAAA TGGTACAAAAAAAGGAAAAACCTGAATTACAGAAAAGTCGAATTTATTTAATGAAAAACT AAAATTAATAAAAATTAGCCNATTCTCACAAAAGAAGCACACAGCAGCACTATGTATTGA CTCACAAAGGGAAAAGCAGTGGCCCATGACCACTTCAGATGGCTTGTCNGTTTAAGGAGA AAAACCCTAACACGCACACTCCCTCGCAGGCTCACACTCCCACGCAAAAACAAACTTTTC AAAATACATCAATCTCCACAAAACAAACTCAGATTATTTACAAGCTTCTTTCTAAACAAA GAGCCATCTGGGTGAAAACAATATTTTCAGGGTTACTGGGCTTATTTAAATATAAGTACT CTGCACGTTCCGGTTAAACCTTTTTTA
Restriction Sites	NotI-NotI
ACCN	AK094663
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK094663.1, BAG52904.1
RefSeq Size	2829 bp
RefSeq ORF	2829 bp
Locus ID	6477
Domains	Sina
Protein Families	Druggable Genome
Protein Pathways	p53 signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway
Gene Summary	This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

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