MTAP (AF109294) Human Untagged Clone

CAT#: SC105031

(untagged)-Human hypothetical methylthioadenosine phosphorylase fusion protein mRNA, complete cds



CNY 11,210.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


MTAP Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 2,700.00

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for AF109294, the custom clone sequence may differ by one or more nucleotides


ATGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTGGATGATCCAG
AAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAGCCATCTGATGCCTTAATTTT
GGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGGCATGGAAGGCAGCACACCATCATGCCTTCA
AAGGTCAACTACCAGGCGAACATCTGGGCTTTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAG
CTTGTGGCTCCTTGAGGGAGGAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGAC
TATTTGCCACGACATTTCAAAGGATTCCAAGAGAGAATATTGGTGTCCATGCTGTGATGATTCCTCAGCT
CCTCTCATCTGA


>OriGene 5' read for AF109294 unedited
NNCCGTTCGAATTTTGTATACGTACTTCACTATAGGGCGGCCGCGAATTCGCACCAGNAC
CACCGCTCTGTGGCTCGCTTGGTTCCCTTAGTCCCGAGCGCTCGCCCACTGCAGATTCCT
TTCCCGTGCAGACATGGCCTCTGGCACCACCACCACCGCCGTGAAGGTGAGATGAGCCCT
CCCAGCCGCAGCGGTTCGCCCTGCCGGATGCCTTCTCGCCCCCGCGCCGGGGGACCGCGC
CTCCGGGGGCCATGCGCCCGGCCCGTGCGTCCCTTGCCGCCGCGGGGAGGGACTGGGGCG
CGGCACTCGGGACTCACTTGCCGCGCGAGGAGAGGATTGGAATAATTGGTGGAACAGGCC
TGGATGATCCAGAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCA
AGCCATCTGATGCCTTAATTTTGGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAA
GGCATGGAAGGCAGCACACCATCATGCCTTCAAAGGTCAACTACCAGGCGAACATCTGGG
CTTTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAGCTTGTGGCTCCTTGAGGG
AGGAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGACCACTATGA
GACCTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAA
TGGCTGAGCCGTTTTGCCCCAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAG
GACTCCGGTGCCACTCAAAGGGGACAATGGTCACCATCGAGGGACCTCGGTTTTAGCTCC
CGGGCAGAAAGCTTCATGTTTCGCACCCTGGGGGCGGGATGTTATCAACTGGACCCCATT
CCAGG
Restriction Sites NotI-NotI     
ACCN AF109294
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AF109294.1, AAD19641.1
RefSeq Size 942 bp
RefSeq ORF 942 bp
Locus ID 4507
Domains Mtap_PNP
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways
Gene Summary This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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