TUBA6 (TUBA1C) (BC033064) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bcm948; TUBA6
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC033064, the custom clone sequence may differ by one or more nucleotides ATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATTGGCAATGCCTGCTGGGAGCTCT ACTGCCTGGAACACGGCATCCAGCCCGATGGCCAGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGA TTCCTTCAACACCTTCTTCAGTGAAACGGGTGCTGGCAAGCATGTGCCCCGGGCAGTGTTTGTAGACTTG GAACCCACAGTCATTGATGAAGTTCGCACTGGCACTTACCGCCAGCTCTTCCACCCTGAGCAACTCATCA CAGGCAAGGAAGATGCTGCCAATAACTATGCCCGAGGGCACTACACCATTGGCAAGGAGATCATTGACCT CGTGTTGGACCGAATTCACAAGTTTGACCTGATGTATGCCAAGCGTGCCTTTGTCCACTTCCCTCTGGCC ACATATGCCCCTGTCATCTCTGCTGAGAAAGCCTACCATGAACAGCTTACTGTAGCAGAGATCACCAATG CTTGCTTTGAGCCAGCCAACCAGATGGTGAAATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCT GTTATACCGTGGTGACGTGGTTCCCAAAGATGTCAATGCTGCCATTGCCACCATCAAAACCAAGCGTACC ATCCAGTTTGTGGATTGGTGCCCCACTGGCTTCAAGGTTGGCATTAATTACCAGCCTCCCACTGTGGTGC CTGGCGGAGACCTGGCCAAGGTACAGAGAGCTGTGTGCATGCTGAGCAATACCACAGCTGTTGCCGAGGC CTGGGCTCGCCTGGACCACAAGTTTGACCTGCCCTTGAGAAGGATTATGAGGAGGTTGGAGCAGATAGTG CTGACGGAGAGGATGAGGGTGAAGAGTATTAACCTGTGTGCTGTACTTTTACACTCCTTTGTCTTGGAAC TGTCTTATTTTTGTTCTGTAAATGTCTATTGCCGTAAATTGTTAATAAAATTGATGTTTCCATTTTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033064 unedited CATTTGTATACGACTTACTATAGGCGGCCGCGAATTCGCACCAGCCGGACTCCTTGGTAG TCTGTTAGTGGGAGATCCTTGTTGCCGTCCCTTCGCCTCCTTCACCGCCGCAGACCCCTT CAAGTTCTAGTCATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATT GGCAATGCCTGCTGGGAGCTCTACTGCCTGGAACACGGCATCCAGCCCGATGGCCAGATG CCAAGTGACAAGACCATTGGGGGAGGAGATGATTCCTTCAACACCTTCTTCAGTGAAACG GGTGCTGGCAAGCATGTGCCCCGGGCAGTGTTTGTAGACTTGGAACCCACAGTCATTGAT GAAGTTCGCACTGGCACTTACCGCCAGCTCTTCCACCCTGAGCAACTCATCACAGGCAAG GAAGATGCTGCCAATAACTATGCCCGAGGGCACTACACCATTGGCAAGGAGATCATTGAC CTCGTGTTGGACCGAATTCGCAAGCTGGCTGACCAGTGCACCGGTCTTCAGGGCTTCTTG GTTTTCCACAGCTTTGGTGGGGGAACTGGTTCTGGGTTCACCTCGCTGCTCATGGAACGT CTCTCAGTTGATTATGGCAAGAAGTCCAAGCTGGAGTTCTCCATTTACCCGGCGCCCCAG TTTTCCACAGCTTGGTGTTGAGCCCTACAACTCCATTCCTACCACCCACACCACCCTGGA ACACTCTGATTGTGCCCTTCATGGTAAACATTGAGGCCATCTATGACATCTGTCGGAGGA ACCTCGTTATTGAGCGCCCAACTACACTTAACCTTAACCGCTTATTAGCCAGATTGGGTC CTCCATAATGGTTCCTAGGATTTGATGGACCCCTGTAATGTGACCTGGCAGAATTCCAGA ACCAACTGGAGCCCAACCCCCGGAACCATTTCCTTATGCCCCTAATGCCCTGTAATTATT GGTGAAAACGCGCATGACACGGTATTGTCGACAAAT
Restriction Sites	NotI-NotI
ACCN	BC033064
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033064.1, AAH33064.1
RefSeq Size	1070 bp
RefSeq ORF	978 bp
Locus ID	84790
Domains	tubulin
Protein Pathways	Gap junction, Pathogenic Escherichia coli infection
Gene Summary	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[UniProtKB/Swiss-Prot Function]

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