BAIAP2L1 (AK074419) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IRTKS
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK074419, the custom clone sequence may differ by one or more nucleotides GAACACGACGTGTCCAAGGCGAGGGGTTGGTTCCCGTCGTCGTACACGAAGTTGCTGGAAGAAAATGAGA CAGAAGCAGTGACCGTGCCCACGCCAAGCCCCACACCAGTGAGAAGCATCAGCACCGTGAACTTGTCTGA GAATAGCAGTGTTGTCATCCCCCCACCCGACTACTTGGAATGCTTGTCCATGGGGGCAGCTGCCGACAGG AGAGCAGATTCGGCCAGGACGACATCCACCTTTAAGGCCCCAGCGTCCAAGCCCGAGACCGCGGCTCCTA ACGATGCCAACGGGACTGCAAAGCCGCCTTTTCTCAGCGGAGAAAACCCCTTTGCCACTGTGAAACTCCG CCCGACTGTGACGAATGATCGCTCGGCACCCATCATTCGATGAGAGGACAGCCAAGGACTCTCCCGGGCC TCTCCGGTTCTCCCTTGCGGAATGATGGGCGCATCCTGTCTGCCACGTGCTGACGGTCGGGAAGCTTCAG TGGAGAGGCCTAACTCTAATGTCGCCTGCTTAAGCAAATCATGCTTCTCTGTTTCACGTAGTTGGGTTGA CAAGTTTCTGCCTTTAAGATAAATGAGTAATAGTCTAATGACCAGCTCAGCCATTTAAAATATTTTCTTC CTATTCTGTTCAAGAAACAGTAAACTTGGTTTCAATCTTTACTGTATTTTTTAAATGAATTTTTTCCTTA ATAACAGCCAGAATAAGGGATAGTCTATGCTTTCAGGACTGGCTTTCTGCACCTGATATGAATGAGACCA GTTTTATTTTATAAAGCATGTGCTCTTAATAGCATTATGTCTAAAGAAGATATCACGTAAGTTTGCATCT TAGCATGCAAATCATAATTTTAAGCAATATAAATTATGAAAATACTATATAAATGTAATTTAACTTAAAA TGTTTAAGTGTAGAGCTTCCAGAGATGGAGAAACCCCCACCCTCCCTCCAACCACGCCAGAGCTGTAGAG TGCTAAGACGCTTTGCCTGCCCTTATCACAGCCACACGTAGCACTCGACGAAATCTCCTCCGAGAGCTCC TTTTCTTGGTAGTTAGGAGTCGCCAACGGATTCGCTGCACACGGCCCATCCAGGACACAGGCCTGGGCGT CGGAGTCACGGCTCCTTGTGTTCTCACTCAGCTAGTTGGCCAAAGCTGCATCCATTACCAGTACACTCAA ACTGCTCGGGGTCCTGGTTTCTCCGTGTTTGAATCAGGTACAGACTCTCAAAGGATACGTGTAAAGCCAG GATGACCTCTGGTGCCTGGAGAAGGGCGGGTTTTTCAGCCTAGCAGCCATGATGCCCTCGGAACCTGGCC CTATGGTATGGATGTGAGGACTACGCACTGGCTGCCCTGAGCCCGGGGCTGGAAATCATCTTTGGCTGCC AAGGATCTGGACCTATTTTGGAGTGGAGAGTCATGGTTAAAATTCCCAGCCCGGCCCAGGTACGGGAATC CCAGCATTTTGTGAGGCCGAGGCAGGGGGATCACCTGAGGTCAGGAGTCTCTACTAAAAATACAAAAATT AGACAGGTGTGGTGGTGGGCGCCACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGCAGAG GTTGCAGTGAGCCAGATCATGCTGCTGCACTCCAGCCCGGCCGCTCACCGTGTGTGTTGCTGGGTGCTGG GGCTGTGACTTATCCCCTCTCCTTTAGCCTTGCCATAAGTGTAGTATCCTATGAGGCTGAGATTGGGAAA GGTTACATGCAGGTAAGCCAGTGGACGTGGCCGATGCTTCAGGCTCCTTCCAGCCAGGTCCAGCAGTGTT ACCATCTGCTTCTCCTGGGAGGACAAACCAGGCACCCCCACCATGAAGTGGCTGCAGGCACCATGAACTA TGTTAACAACCCCAGTCTGTACTACAGAAAGGGCTGCAGCCACATGAGAATTCAGTCCACACAAGCCCCA TGGCCGTGTTCCCCACTTCAGCCACAGGGCTCAGGGAGCCCCATCTGGCGCTAAGGGGAACTGCTGGGGT GTGGGTGACACCTGGCCTTTGGCGTTCTGCCTTGGGGAGGTTTCTGGTTTTGTTACGGGGTGGAAGAATA GGACCTGGGGGTCTCGGATGCAACCTGCAGACCCCGTGGCTCACCCAACCCCAGGTTCTGCCTCCCAGAC CAGAACGGGCATGGCCTGGTCCTTGGCACCGAGGTGCCTGCTCTGTAAATATCAAGGGATTACAACTTTA ATAATAAAGCAGAACTTGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK074419 unedited GGTTCNCAATAGNATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGATATCACGT AAGTTTGCATCTTAGCATGCAAATCATAATTTTAAGCAATATAAATTATGAAAATACTAT ATAAATGTAATTTAACTTAAAATGTTTAAGTGTAGAGCTTCCAGAGATGGAGAAACCCCC ACCCTCCCTCCAACCACGCCAGAGCTGTAGAGTGCTAAGACGCTTTGCCTGCCCTTATCA CAGCCACACGTAGCACTCGACGAAATCTCCTCCGAGAGCTCCTTTTCTTGGTAGTTAGGA GTCGCCAACGGATTCGCTGCACACGGCCCATCCAGGACACAGGCCTGGGCGTCGGAGTCA CGGCTCCTTGTGTTCTCACTCAGCTAGTTGGCCAAAGCTGCATCCATTACCAGTACACTC AAACTGCTCGGGGTCCTGGTTTCTCCGTGTTTGAATCAGGTACAGACTCTCAAAGGATAC GTGTAAAGCCAGGATGACCTCTGGTGCCTGGAGAAGGGCGGGTTTTTCAGCCTAGCAGCC ATGATGCCCTCGGAACCTGGCCCTATGGTATGGATGTGAGGACTACGCACTGGCTGCCCT GAGCCCGGNGCTGGAAATCATCTCTGGCTGCCAAGGATCTGGACCTATTTTGGAGTGGAG AGTCATGGTTAAAATTCCCAGCCCGGCCCANGTACCGGGATCCCAGCATTTTGTGAGGCC CGAGCAGGGGGATCACCTNGAGTCAGGAGTCTCTACTNAAAATACAAAAATTAGACAGGT GTGGTGGTGGGCGCCTCAGGGAGGGGCTTGGNNANNANAATAAATTTTAACCCGNGGAGG AAAAATTTTTATTGAGCCCCATAATAGGTTGTTGGCCTCCACCCCCGGCCGTCTCCCTGT GGGGGTTCGCGGGGAGCTGGGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK074419 unedited NAAGGTCTACTATGTACGGGCCGCTTTTNAGATCGGTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGTTTTCAAATTCTGCTTTATTATTAAA GTTGGAATCCCTTGGATATTTACAAAACAAGCACCTTGGTGCCAAAGACCAAGCCATGCC CGTTTTGGTTTGGGAGGCAAAACCTGGGGTTGGGTGAACCACGGGGTCTGCAGGTTGCAT CCGAAACCCCCAGGTCCTATTTTTCCACCCCGTAACAAAACCAAAAACCTCCCCAAGGCA AAACGCCAAAGGCCAGGTGTCACCCACACCCCAACAGTTCCCCTTAACGCCAAATGGGGC TCCCTGAACCCTGTGGCTGAAGTGGGGAACACGGCCATGGGGCTTGGGGGGACTGAATTC TCATGTGGCTGCAGCCCTTTTTGTAGTACAAACTGGGGTTGTTAACATAATTCAAGGGGC CTGCAGCCCCTTCATGGGGGGGGTGCCTGGTTTGTCCTCCCAGGAAAAACAAATGGTAAC ACTGCTGGACCTGGCTGGAAAGAACCTGAAACATCGGCCACGTCCACTGGCTTACCTGGA TGTAACCTTTCCCAATCTCAACCTCATAGGATACTACACTTATGGCAAGGCTAAAGGAGA GGGGGTAAGTCAAAACCCCAGCACCCAGCAACACACACGGTGAACGGCCGGGCTGGGAAT GCANCACATGATCCTGGGCTCACTGCAACCTCTGCTCCCGGGGTTAAAAAGTGATTTTCC CGGCCTAAACCCTCCTGAATGGCGCCCACCACCACACCTGGCTAATTTTTTGGATTTTAA GAAAAGACTCCGGGACCTCAGGGAT
Restriction Sites	NotI-NotI
ACCN	AK074419
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK074419.1
RefSeq Size	2296 bp
RefSeq ORF	2296 bp
Locus ID	55971
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the IMD (IRSp53/MIM homology domain) family. Members of this family can be subdivided in two groups, the IRSp53-like and MIM-like, based on the presence or absence of the SH3 (Src homology 3) domain. The protein encoded by this gene contains a conserved IMD, also known as F-actin bundling domain, at the N-terminus, and a canonical SH3 domain near the C-terminus, so it belongs to the IRSp53-like group. This protein is the substrate for insulin receptor tyrosine kinase and binds to the small GTPase Rac. It is involved in signal transduction pathways that link deformation of the plasma membrane and remodeling of the actin cytoskeleton. It also promotes actin assembly and membrane protrusions when overexpressed in mammalian cells, and is essential to the formation of a potent actin assembly complex during EHEC (Enterohemorrhagic Escherichia coli) pedestal formation. [provided by RefSeq, Oct 2009]

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