CHMP2A (AK092868) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BC-2; BC2; CHMP2; VPS2; VPS2A
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092868, the custom clone sequence may differ by one or more nucleotides AAAACGGGTGTCCGTCCCAGCTCCGGTGAGTGGCCCCTGCTGGACCGTGGATCCCGGGCGGGCGTGCGGG CGGCAGCGTACGAGGCCTTCTACTCGCCCGGGTGTCCGGTGCCTCGCTTCTCCCTGGGACCCTGTCGTCA ACATCGAGGTCTCCAACACCACCTTGCTTTGGTGCCGGGCCCACTGACTGTCCTCGGTGTATAGTGCGAC CGAATCTCCATCATCCCGTCCTCAGTGTACAGGATGCCCCAGCGCCCCCAACCGTCTCCCTCAGCATTCA GGGTCCCCAAATCCTAATGCCTTGTCCTGAGCCTCACAGCCCCACATTCACCTGTTCTTGGTACTTAGCG TTTCCGCCCATCCTGTCCTCCTCTATCAGAACCCCCACAATTGCTCTGTCCTCGGTGCCCACAGTCTCCA AAACACCGGTGCACCGGCCCGCGTCATCCCCCGCTCTCAGTCGTATCAAGGACTCCAGGGCCTCACCCAT AGTTCTGGAGAGGGAAGCTGAACTTGCAAGCCATAACTTCTTTTGGGGCATGGCGATGAAGGAGGGGCTC GGGCCAGCACTTGGATAATTTTCAGGTCTACCTGTCCCCCTTTATGTGTCCCCGGGACAATCCCAGTGTT CTCAGCTGCTCCCTTTTGGCTCCTCATTCCTGCCTCCAGTCTCCGTTAGCCCCTTCCCATTTGGCAAGGC TCCCGTGACCCTGTTCTGCTTCTTGCCCTCCTGGGCCCCATGCCCTACAACTGGGCCTCACTCACATCTG TCTCAGGCCTGCCAGTGAGCTTCTACCATCATGGACCTATTGTTCGGGCGCCGGAAGACGCCAGAGGAGC TACTGCGGCAGAACCAGAGGGCCCTGAACCGTGCCATGCGGGAGCTGGACCGCGAGCGACAGAAACTAGA GACCCAGGAGAAGAAAATCATTGCAGACATTAAGAAGATGGCCAAGCAAGGCCAGATGGTGAGTGTGGGT GGTCGGGCAGGTTGCTGTCAATCAGGCACGGCTCTAACAGCAGTGAACAAGAGAGACCAGACACCAAACA CAGGTGCTTTCAGCAAAACGAGTGGAGTGAAAGGTCAGCAGGGCTTCCCTAGTTTGAGGGTCTGGAAGGC TGCCTCCAAGGAACTGGTATGAGATCCAAGGCTTGAGTGATGAGAGTGAGCCTACAGATCTGGGCAGGGA ATGTGTTAAGCAGACAGAGCAGCAAATGTTAAAAAAGAGCCTCAAAAAAATGGGAGATGACAGGTGAGCT TCAAGAGTTGGGCATGGACCAGACAATTCAGAACTCTGGGCCAAAGTGGGAGCTAAGTTGGAAGGTTAGG GATTACAGGGCCGGGCGCAGTAGCTCAGAGACCTGTAATCCCAGCACTTTGGGAGGCAGAGGTGGTTGGA TCACCTGAGGTCAGGACTTCGAGACCAGCCTGACCAACACAGAGTAACCCCGTCTCTACTGAAAATAAAA AAAACTAGCCAGGCATGGTGGCGCACGCCTGTAATCCCAGCTACTCAGCAGGGCTGAGGCAGGACAATCT CTTGAACCCAGGAGGCGGAGGTTACAGTGAGCCAAGATCATGCCACTGCACTCCAGCCTGGGTGACAGAG CGAGACTCTGTCTCAAAAAAAAAAAAAAAAAAACCAGTTAACTATAAGGATAAGCTGGCCCGGCATGATG GCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATCACCTGAGGTCAGGATTTCGAGA CCAGCCTGGCCAACATGGCAAAACCCCACTCTACCAAAAATTAGCCGGGCATGGTGGTGCATACTTGTTA TCCCAGCTATTCGAGAGGCTGAGGCAGAAGAATCGCTTGAGGCCGGGAGGCACAGTTTGCAGTGAGCCAA GATTGTGCCACTGCACTCCAGCCTAGGCAACAAAGCAAGACTCCAACTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092868 unedited CCCCCCCACCCCACCNNNNCCACCCCCCCCGTTCAAATTTGTATACGACTCATATAGGCG GCCGCGNAATTCGCGGCGGCGACAGGACCGAGGGGCCTTAGTTGGTGGGCAAGTCGGGGA TCCCAGAAAGAGAAGCGTGACCCGGAAGCGGAAACGGGTGTCCGTCCCAGCTCCGGCCTG CCAGTGAGCTTCTACCATCATGGACCTATTGTTCGGGCGCCGGAAGACGCCAGAGGAGCT ACTGCGGCAGAACCAGAGGGCCCTGAACCGTGCCATGCGGGAGCTGGACCGCGAGCGACA GAAACTAGAGACCCAGGAGAAGAAAATCATTGCAGACATTAAGAAGATGGCCAAGCAAGG CCAGATGGTGAGTGTGGGTGGTCGGGCAGGTTGCTGTCAATCAGGCACGGCTCTAACAGC AGTGAACAAGAGAGACCAGACACCAAACACAGGTGCTTTCAGCAAAACGAGTGGAGTGAA AGGTCAGCAGGGCTTCCCTAGTTTGAGGGTCTGGAAGGCTGCCTCCAAGGAACTGGTATG AGATCCAAGGCTTGAGTGATGAGAGTGAGCCTACAGATCTGGGCAGGGAATGTNGTAAGC AGACAGAGCAGCANATGTTAAAAAAGAGCCTCANAAAATGGGAGATGACAGGTGAGCTTC AAGAAGTGGGCATGGACCAGACANTNCAGAAACTCTGGCCCAAAGTGGGAGCTAAGTTGG AAGGTTAGGGGATACAGGGCCCGGNCGCAGTAGCTCAGAGACCTGTAATCCCAGCACTTG GAGGCAGAAAGGGGGGNNNAAAAACCNAAAGNAAGGAAATTTAAACCAANCTGACCCACC CAAAAAAACCCCCGGTTTTACGGAACAAAAAAACAACCACCCGCGCNTGGGGGCCCCCCC CCGTACCCCCAATTCCTCAAAAGCTTGGGGCGTGGAAATTTTTGTTACCCATAGGCGGGG GGTCCCCCGCCCCTAAATCTCCCATTCGCCCCCCTCTTGGGGTACAACCCCATCCTTCTC CAAAAAAAT
Restriction Sites	NotI-NotI
ACCN	AK092868
Insert Size	1000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK092868.1
RefSeq Size	1939 bp
RefSeq ORF	1939 bp
Locus ID	27243
Protein Pathways	Endocytosis
Gene Summary	CHMP2A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]

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