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EEF1D (NM_032378) Human Untagged Clone
CAT＃: SC104703
EEF1D (untagged)-Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 1
CNY 10,360.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EF-1D; EF1D; FP1047 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_032378, the custom clone sequence may differ by one or more nucleotides
ATGAGGAGCGGGAAGGCCTCCTGCACCCTGGAGACCGTGTGGGAAGACAAGCACAAGTATGAGGAGGCCG AGCGGCGCTTCTACGAACACGAGGCCACACAGGCGGCCGCCTCCGCCCAGCAGCTGCCAGCCGAGGGGCC AGCCATGAATGGGCCCGGCCAGGACGACCCTGAGGACGCTGATGAGGCGGAAGCCCCTGACGGCGGCAGC AGGCGTGATCCCAGGAAGAGCCAGGACAGCAGGAAGCCCCTGCAGAAAAAGAGGAAGCGCTCCCCCAAGA GCGGGCTCGGCCCCGCGGACCTGGCCCTCCTGGGCCTCTCGGCCGAACGCGTGTGGCTGGACAAGTCACT TTTCGACCAGGCAGAGAGCTCCTACCGCCAGAAGCTGGCAGATGTGGCTGCCCAGGCAGCCTGGCCTCCT GCCTTGGCCCCTTGGGGTCTCTGCACCCATGGAAACCAGGTGGCCTGCCACCACGTGACCTGGGGGATCT GGGTCAACAAGTCCTCCTTCGACCAGGCTGAGCGGGCCTTCGTGGAGTGGTCTCAGGCCCTGTTGCTGGC CCCCGAGGGCAGCCGCAGGCAGGGGACTCCCAACACAGGCCAGCAGGTGGCCGTCCCCGACCTGGCCCAC CAGCCCAGCCCACCGGTCAATGGCCAGCCCCCGCTGGGCAGCCTGCAGGCACTGGTTCGGGAGGTGTGGC TGGAGAAGCCCCGGTATGATGCAGCCGAGAGGGGCTTCTACGAGGCCCTGTTTGACGGCCATCCCCCAGG GAAGGTGCGCCTGCAAGAGCGAGCCGGCCTGGCCGAGGGTGCCCGGCGGGGCCGCAGAGACCGGCGGGGC CGCAACATCTTAGGGAACAAGCGGGCCGGGCTGCGACGGGCCGATGGGGAGGCCCCCTCTGCCTTGCCCT ACTGTTACTTCCTGCAGAAGGATGCAGAGGCCCCCTGGCTCAGCAAGCCTGCCTACGACAGCGCCGAGTG CCGCCACCACGCTGCCGAGGCCCTGCGGGTGGCCTGGTGCCTCGAAGCTGCCTCCCTGTCTCACCGACCC GGTCCTCGGTCTGGCCTGTCCGTGTCCAGCCTGAGACCCAACAGAAAAATGGCTACAAACTTCCTAGCAC ATGAGAAGATCTGGTTCGACAAGTTCAAATATGACGACGCAGAAAGGAGATTCTACGAGCAGATGAACGG GCCTGTGGCAGGTGCCTCCCGCCAGGAGAACGGCGCCAGCGTGATCCTCCGTGACATTGCGAGAGCCAGA GAGAACATCCAGAAATCCCTGGCTGGAAGCTCAGGCCCCGGGGCCTCCAGCGGCACCAGCGGAGACCACG GTGAGCTCGTCGTCCGGATTGCCAGTCTGGAAGTGGAGAACCAGAGTCTGCGTGGCGTGGTACAGGAGCT GCAGCAGGCCATCTCCAAGCTGGAGGCCCGGCTGAACGTGCTGGAGAAGAGCTCGCCTGGCCACCGGGCC ACGGCCCCACAGACCCAGCACGTATCTCCCATGCGCCAAGTGGAGCCCCCAGCCAAGAAGCCAGCCACAC CAGCAGAGGATGACGAGGATGATGACATTGACCTGTTTGGCAGTGACAATGAGGAGGAGGACAAGGAGGC GGCACAGCTGCGGGAGGAGCGGCTACGGCAGTACGCGGAGAAGAAGGCCAAGAAGCCTGCACTGGTGGCC AAGTCCTCCATCCTGCTGGATGTCAAGCCTTGGGATGATGAGACGGACATGGCCCAGCTGGAGGCCTGTG TGCGCTCTATCCAGCTGGACGGGCTGGTCTGGGGGGCTTCCAAGCTGGTGCCCGTGGGCTACGGTATCCG GAAGCTACAGATTCAGTGTGTGGTGGAGGACGACAAGGTGGGGACAGACTTGCTGGAGGAGGAGATCACC AAGTTTGAGGAGCACGTGCAGAGTGTCGATATCGCAGCTTTCAACAAGATCTGA >OriGene 5' read for NM_032378 unedited
ACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCGCGTCCGCCGA TTCCTCCTCCTTGGTCGCCGCGTCCTTGGCTGGCGTGAGGCCAAAGCAAAATGAGGAGCG GGAAGGCCTCCTGCACCCTGGAGACCGTGTGGGAAGACAAGCACAAGTATGAGGAGGCCG AGCGGCGCTTCTACGAACACGAGGCCACACAGGCGGCCGCCTCCGCCCAGCAGCTGCCAG CCGAGGGGCCAGCCATGAATGGGCCCGGCCAGGACGACCCTGAGGACGCTGATGAGGCGG AAGCCCCTGACGGCGGCAGCAGGCGTGATCCCAGGAAGAGCCAGGACAGCAGGAAGCCCC TGCAGAAAAAGAGGAAGCGCTCCCCCAAGAGCGGGCTCGGCCCCGCGGACCTGGCCCTCC TGGGCCTCTCGGCCGAACGCGTGTGGCTGGACAAGTCACTTTTCGACCAGGCAGAGAGCT CCTACCGCCAGAAGCTGGCAGATGTGGCTGCCCAGGCAGCCTGGCCTCCTGCCTTGGCCC CTTGGGGTCTCTGCACCCATGGAAACCAGGTGGCCTGCCACCACGTGACCTGNGGGATCT GGGTCAACAAGTCCTCCTTCGACCAGGCTGAGCGGGCCTTCGTGGAGTGGTCTCAGGCCC TGTTGCTGGCCCCCGNAGGCAGCCGCAGGCAGGGGACTCCCAACACAGGCCAGCAGGTGG CCGTCCCCGACCTGGCCCACCAGCCCAGCCCACCGGTCAATGGCCAGCCCCCGCTGGGCA GCCTGCAGGCACTGGTTCGGNGAGTGTGGCTGGNAGAGCCCCGGTATGATGCAGCCGAGA GGGCTTCTACGAGCCCTGTTTGACGCCATCCCCAGGGAAGGTGCGCTGCANAACGAGCCC GCCTGCCGAGTGCCCCGCGGGCCGCAANACCGCGNGCCGCACATCTAGGAACAAGCGGGC GCT >OriGene 3' read for NM_032378 unedited
NNNTTTTGACTTGGCCCGGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTGCCGGGCTCAATCTTTAATCGGGGGAGGGCCTTACGC ACGCGCGCACGTACACACACTTAGGCTTTAAATCTTGGTGAAAGCTGGGATATCGACACT TTGGACGGGCTCCTCAAACTTGGGGATCTCCTCCTCCAGCAAGGCTGGCCCCACCTTGGC GTCCTCCACCACACACTGAATCTGGAGCTTCCGGATACCGGAACCCACGGGCACCAGCTT GGAAGCCCCCCAAACCAACCCGTCCAGCTGGATAAAGCGCACACAGGCCTCCAGCTGGGC CATGTCCGTCTCATTATCCCAAGGGTTGACATCCAGCAGGATGGAGGACTTGGCCACCAG TGCAGGCTTTTTGGCCTTTTTTTCCGCGTACTGGCGGAACCGCTCCTCCCGCAACTGGGC CGCCTCCTTGGCCTCCTCCTCATTGGCACTGCCAAACAGGGCAATGGCATCATCCTCGGC ATCCTCTGGTGGGGGGGCTGGCTTCTTGGCTGGGGGCTCCACTTGGCGCATGGGAAATAC CTGCTGGGTCTGTGGGGCCGTGGCCCGGTGGCCAAGCGAGCTCTTCTTCAGCACGTTCAA CCGGGCCTTCAACTTGGAGAAGGCCTGCTGCAGCTCCTGTACCACGCCACGCAGACTCTG GTTCTTCACTTCCAGACTGGCAATCCGGACGACGAGCTCACCGTGGTCTTCCCTGGTGCC GCTGGAAGCCCCGGGGCCTGAACCTCCCACCAGGGATTCTGGGAGGTCTCTTTGGCCTTC GCAAGTACGGAGGATACCCTTGGGCCGGTCTTCTGGGGGGGAGGACCTGCACCAGGCCGT CATTTGCTTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032378 |
| Insert Size | 1840 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032378.2, NP_115754.2 |
| RefSeq Size | 2216 bp |
| RefSeq ORF | 1944 bp |
| Locus ID | 1936 |
| UniProt ID | P29692 |
| Domains | EF1BD |
| Gene Summary | This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 3 both encode the same isoform (1, also known as L). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224856 | EEF1D (Myc-DDK-tagged)-Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 1 |
CNY 4,816.00 |
|
| RC224856L3 | Lenti ORF clone of Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC224856L4 | Lenti ORF clone of Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG224856 | EEF1D (tGFP-tagged) - Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 1 |
CNY 5,040.00 |
