GLYCTK (AK074215) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HBeAgBP4A; HBEBP2; HBEBP4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK074215, the custom clone sequence may differ by one or more nucleotides ATGCTGCTGAAGCCACATAGCCGTGTCCAGGTATTCGAGGGTGCGGAGGACAACCTCCCGGACCGCGATG CGCTGCGGGCTGCACTGGCCATCCAGCAACTGGCTGAGGGACTCACAGCTGATGACCTGCTGCTCGTGCT GATCTCAGGTGGGGGTTCAGCTCTGCTGCCTGCCCCCATCCCACCTGTCACACTGGAGGAGAAGCAGACA CTCACTAGACTGCTGGCAGCCCGTGGAGCCACCATCCAGGAGTTGAACACCATTCGGAAGGCCCTGTCCC AGCTCAAGGGTGGGGGGCTGGCTCAGGCCGCCTACCCTGCCCAGGTGGTGAGCCTCATCCTGTCAGATGT GGAGGGGGACCCTGTGGAGGTGATTGCCAGTGGCCCCACCGTGGCCAGTTCCCACAATGTGCAAGATTGC CTGCATATCCTCAATCGATACGGCCTCCGTGCAGCCCTGCCACGTTCTGTGAAGACTGTGCTGTCTCGGG CCGACTCTGACCCCCATGGGCCACACACCTGTGGCCATGTCCTGAATGTGATCATTGGCTCTAATGTGCT GGCGCTAGCTGAGGCCCAGCGGCAGGCCGAGGCACTGGGCTACCAGGCTGTGGTGCTGAGTGCAGCCATG CAAGGGTGGGGCACACCTGCTGCACACAGGGATGACAGGTACCAATGTCATGGACACCCACCTATTGTTC CTGCGGCCTCGGTGATGGCATAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK074215 unedited NNCCGTTCACATATTTGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACGAGTGGGG GACCTCCTGTCTTTTGCCTTGCAAGGGCCTCAGTTGTGCTTTTTCCCTCTAGGCAGCCAT GGGTGCCAGGCAGTGCTGAGAGCAGTGGGGCATGGCTGCAGCCCTGCAGGTCCTGCCCCG CTTGGCCCGAGCCCCCTTGCATCCACTCCTCTGGCGGGGCTCAGTGGCCCGTCTGGCCAG CAGCATGGCCTTGGCAGAGCAGGCCAGGCAGCTGTTTGAGAGTGCTGTAGGTGCAGTGCT GCCGGGCCCCATGCTGCACCGGGCACTATCCTTGGACCCTGGTGGCAGACAGCTGAAGGT GCGGGACCGGAACTTTCAGCTGAGGCAAAACCTCTACCTGGTGGGCTTTGGCAGGGCTGT GCTGGGTATGGCAGCTGCAGCTGAGGAACTACTGGGCCAGCATCTTGTGCAGGGCGTGAT CAGCGTTCCCAAGGGGATCCGTGCTGCCATGGAGCGTGCCGGCAAGCAGGAGATGCTGCT GAAGCCACATAGCCGTGTCCAGGTATTCGAGGGTGCGGAGGACAACCTCCCGGACCGCGA TGCGCTGCGGGCTGCACTGGCCATCCAGCAACTGGCTGAGGGACTCACAGCTGATGACCT GCTGCTCGTGCTGATTCTCAGTGGGGGGTTCAGCTCTGCTGCCTGCCCCCATCCCACCTG TCACACTGGAGGAGAAGCAGACACTCACTAGACTGCTGGCAGCCCGTGGAGCCACCATCC AGGAGTTGAACACCATTCGGAAGGCCCTGTCCCAGCTCAAGGGTGGNGNGGGCTGGGCTA AGGCCGCCTACCCTGCCCAGGTGGTGAGCCTCATCCTGTCAGATGTGGGTGGGGGACCCT GTGGAAGGTGAATTGCAAGTG
Restriction Sites	NotI-NotI
ACCN	AK074215
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK074215.1, BAB85018.1
RefSeq Size	1012 bp
RefSeq ORF	723 bp
Locus ID	132158
Protein Families	Transcription Factors
Protein Pathways	Glycerolipid metabolism, Glycine, serine and threonine metabolism, Glyoxylate and dicarboxylate metabolism, Metabolic pathways
Gene Summary	This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

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